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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Cardiovascular and Smooth Muscle Pharmacology
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1394557
This article is part of the Research Topic Nutraceuticals in Cardiovascular Diseases and their Associated Risk Conditions View all 9 articles

Alchemilla vulgaris modulates isoproterenol-induced cardiotoxicity: Interplay of oxidative stress, inflammation, autophagy, and apoptosis

Provisionally accepted
Nuha Anajirih Nuha Anajirih 1Ahmed Abdeen Ahmed Abdeen 2*Ehab S. Taher Ehab S. Taher 3Afaf Abdelkader Afaf Abdelkader 2Hoda A. Abdellatieff Hoda A. Abdellatieff 4Mahmoud S. Gewaily Mahmoud S. Gewaily 5Nashwa E. Ahmed Nashwa E. Ahmed 2Rasha AlSerwi Rasha AlSerwi 6Safwa M. Sorour Safwa M. Sorour 2Heba M. Abdelkareem Heba M. Abdelkareem 2Heba M. Abdelkareem Heba M. Abdelkareem 2Elturabi Ebrahim Elturabi Ebrahim 7Mohamed El-Sherbiny Mohamed El-Sherbiny 8Imbrea Florin Imbrea Florin 9Ilinca Imbrea Ilinca Imbrea 10*Ola Habotta Ola Habotta 8
  • 1 Umm al-Qura University, Mecca, Saudi Arabia
  • 2 Benha University, Benha, Qalyubia, Egypt
  • 3 Al-Azhar University, Cairo, Cairo, Egypt
  • 4 Damanhour University, Damanhour, Egypt
  • 5 Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Kafr el-Sheikh, Egypt
  • 6 Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
  • 7 Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia
  • 8 Mansoura University, Mansoura, Dakahlia, Egypt
  • 9 Banat University of Agricultural Sciences and Veterinary Medicine, Timișoara, Romania
  • 10 Faculty of Engineering and Applied Technologies,University of Life Science 'King Mihai I', Timisoara, Romania

The final, formatted version of the article will be published soon.

    Isoproterenol (ISO) is regarded as an adrenergic non-selective β agonist. It regulates myocardial contractility and may cause damage to cardiac tissues. Alchemilla vulgaris (AV) is an herbal plant that has garnered considerable attention due to its anti-inflammatory and antioxidant bioactive components.The present investigation assessed the cardioprotective potential of AV towards ISO-induced myocardial damage. Four groups of mice were utilized: control that received saline, an ISO group (85 mg/kg, S.C.), ISO+AV100, and ISO+AV200 groups (mice received 100 or 200 mg/kg AV orally along with ISO). ISO induced notable cardiac damage demonstrated by clear histopathological disruption and alterations in biochemical parameters. Intriguingly, AV treatment mitigates ISO provoked oxidative stress elucidated by a substantial enhancement in superoxide dismutase (SOD) and catalase (CAT) activities and reduced glutathione (GSH) content, as well as a considerable reduction in malondialdehyde (MDA) concentrations. In addition, notable down-regulation of inflammatory biomarkers (IL-1β, TNF-α, and RAGE) and the NF-κB/p65 pathway was observed in ISO-exposed animals following AV treatment. Furthermore, the pro-apoptotic marker Bax was downregulated together with autophagy markers Beclin1 and LC3 with in ISO-exposed animals when treated with AV. Pre-treatment with AV significantly alleviated ISO-induced cardiac damage in a dose related manner, possibly due to their antioxidant and anti-inflammatory properties. Interestingly, when AV was given at higher doses, a remarkable restoration of ISO-induced cardiac injury was revealed. Table 1. Molecular docking interaction of Alchemilla vulgaris's bioactive compounds against TNF-α, IL-1β, NF-κB1, Bax, RAGE, and HMBG1 Ligands Binding Free Energy (kcal/mol) TNF-α IL-1β NF-κB1 Bax RAGE HMBG1

    Keywords: Alchemilla vulgaris, Isoproterenol, Myocardial injury, Oxidative Stress, inflammatory cytokines, HMBG1/RAGE pathway

    Received: 01 Mar 2024; Accepted: 15 Jul 2024.

    Copyright: © 2024 Anajirih, Abdeen, Taher, Abdelkader, Abdellatieff, Gewaily, Ahmed, AlSerwi, Sorour, Abdelkareem, Abdelkareem, Ebrahim, El-Sherbiny, Florin, Imbrea and Habotta. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Ahmed Abdeen, Benha University, Benha, 13518, Qalyubia, Egypt
    Ilinca Imbrea, Faculty of Engineering and Applied Technologies,University of Life Science 'King Mihai I', Timisoara, 300645, Romania

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