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ORIGINAL RESEARCH article

Front. Pharmacol.
Sec. Experimental Pharmacology and Drug Discovery
Volume 15 - 2024 | doi: 10.3389/fphar.2024.1392385

The energy metabolism-promoting of aconite is associated with gut microbiota and bile acid receptor TGR5-UCP1 signaling

Provisionally accepted
Dandan Zhang Dandan Zhang 1,2Hao Cheng Hao Cheng 1,2Jing Wu Jing Wu 1,2Yaochuan Zhou Yaochuan Zhou 2Wuwen Feng Wuwen Feng 2*Cheng Peng Cheng Peng 1,2*
  • 1 Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, China
  • 2 School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan Province, China

The final, formatted version of the article will be published soon.

    Abstract Introduction: As a widely used traditional Chinese medicine with hot property, aconite can significantly promote energy metabolism. However, it is unclear whether the gut microbiota and bile acids contribute to the energy metabolism-promoting of aconite. The aim of this experiment was to verify whether the energy metabolism-promoting of aconite aqueous extract (AA) is related to gut microbiota and BA metabolism. Methods: The effect of AA on energy metabolism in rats was detected based on body weight, body temperature, adipose tissue by HE staining and immunohistochemistry. In addition, 16S rRNA high-throughput sequencing and targeted metabolomics were used to detect changes in gut microbiota and BAs (bile acids) concentrations, respectively. Antibiotic treatment and fecal microbiota transplantation (FMT) were also performed to demonstrate the importance of gut microbiota. Results: Rats given AA experienced a rise in body temperature, a decrease in body weight, and an increased in BAT (brown adipose tissue) activity and browning of WAT (white adipose tissue). Sequencing analysis and targeted metabolomics indicated that AA modulated gut microbiota and BA's metabolism. The promoting energy metabolism of AA was found to be mediated by gut microbiota, as demonstrated through antibiotic treatment and FMT. Moreover, the energy metabolism promoting effect of aconite is associated with bile acid receptor TGR5 (Takeda G-protein-coupled receptor 5)-UCP1 (uncoupling protein 1) signaling pathway. Conclusion: The energy metabolism-promoting of aconite is associated with gut microbiota and bile acid receptor TGR5-UCP1 signaling.

    Keywords: Aconite, Gut Microbiota, Bas, antibiotic, fecal microbiota transplantation, Energy Metabolism

    Received: 27 Feb 2024; Accepted: 18 Jul 2024.

    Copyright: © 2024 Zhang, Cheng, Wu, Zhou, Feng and Peng. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Wuwen Feng, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, Sichuan Province, China
    Cheng Peng, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, Sichuan Province, China

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