AUTHOR=Panklai Teerapap , Ingkaninan Kornkanok , Chootip Krongkarn , Temkitthawon Prapapan , Suphrom Nungruthai , Tournier-Nappey Maude , Girard Corine , Demougeot Céline , Totoson Perle 

TITLE=Vasorelaxant and hypotensive effects of an ethanolic extract of Nymphaea pubescens and its main compound quercetin 3-methyl ether 3′-O-β-xylopyranoside

JOURNAL=Frontiers in Pharmacology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1379752

DOI=10.3389/fphar.2024.1379752

ISSN=1663-9812

ABSTRACT=<p><bold>Aim:</bold><italic>Nymphaea</italic> plants were traditionally used to treat diseases associated with endothelial dysfunction. The present study investigated the effects of an ethanolic extract of <italic>Nymphaea pubescens</italic> Willd. (commonly named water lily, WL) and its main compound <bold>1</bold> (quercetin 3-methyl ether 3′-O-<italic>β</italic>-xylopyranoside) on vascular function in rats.</p><p><bold>Materials and methods:</bold> The vasorelaxant effects of the WL extract and its main compound <bold>1</bold> and their underlying mechanisms of action were evaluated on isolated mesenteric arteries from Wistar rats. Blood pressure and heart rate were measured in anesthetized rats after infusion (i.v) of vehicle, WL extract, and compound <bold>1</bold> (at 0.01, 0.025, 0.05, 0.1, 0.5, and 1 mg/kg). Nifedipine was used as a positive control.</p><p><bold>Results:</bold> Both WL extract and compound <bold>1</bold> induced vasorelaxant effects (with EC<sub>50</sub> of 0.08 ± 0.01 mg/mL and 42.8 ± 6.3 µM, respectively) that were reduced by endothelium removal. A significant decrease in these relaxations was observed with L-NAME but not with apamin–charybdotoxin or indomethacin. In the endothelium-denuded condition, WL extract-induced relaxation was enhanced by 4-aminopyridine and glibenclamide, while iberiotoxin and ODQ (1H-[1,2,4]oxadiazolo[4,3-a]quinoxaline-1-one) had no effect. In contrast, compound <bold>1</bold>-induced relaxation was not changed by any of these inhibitors. Both WL extract and compound <bold>1</bold> enhanced sodium nitroprusside-induced relaxation and inhibited receptor-operated Ca<sup>2+</sup> channels. Only the WL extract was able to reduce PE-induced contraction (<italic>p</italic> &lt; 0.001). As compared to the vehicle, the infusion of WL extract and compound <bold>1</bold> lowered systolic and diastolic blood pressure. Interestingly, the hypotensive effect of the compound was similar to that of nifedipine. The rebound tachycardia found at the highest dose of nifedipine was not observed with the WL extract or compound <bold>1</bold> (<italic>p</italic> &lt; 0.05).</p><p><bold>Conclusion and discussion:</bold> Our study demonstrated a vasorelaxant effect of the WL extract and its main compound quercetin 3-methyl ether 3′-O-<italic>β</italic>-xylopyranoside, relying on the potentiation of the NO-cGMP pathway and calcium inhibitory effects. These vasorelaxant effects were associated with a potent hypotensive effect, providing pharmacological evidence for the traditional use of this plant.</p>