AUTHOR=Herenda Safija , Carev Ivana , Haskovic Denis , Prevljak Sabina , Causevic Sara , Haskovic Edhem 

TITLE=Glucocorticoids influence on rat hematological parameters and catalase activity

JOURNAL=Frontiers in Pharmacology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1367350

DOI=10.3389/fphar.2024.1367350

ISSN=1663-9812

ABSTRACT=<p>In this study, the impact of glucocorticoid, betamethasone dipropionate on enzyme activity <italic>in vitro</italic> and its effects on hematological parameters <italic>in vivo</italic> was investigated. The immobilized catalase, crucial for cell oxidative stress response via hydrogen peroxide reduction, exhibited a robust electrocatalytic response, maintaining its biological activity. The <italic>in vitro</italic> inhibition kinetics of catalase, as determined by electrocatalytic methods and expressed using Lineweaver-Burke diagrams, revealed an uncompetitive type of inhibition with altered Imax and Km in the presence of a range of betamethasone dipropionate concentrations. The <italic>in vivo</italic> experiments conducted on <italic>Rattus norvegicus</italic> demonstrated significant alterations in hematological parameters following betamethasone dipropionate administration. These changes included a decrease in erythrocyte count, an increase in hemoglobin, a reduction in mean corpuscular volume (MCV), and an elevation in mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). Notably, the leukocyte counts substantially increased. The observed hematological shifts suggest an impact of betamethasone dipropionate on the hematopoietic system, reinforcing the need for cautious corticosteroid administration. The findings underline the necessity for judicious corticosteroid treatment, acknowledging both enzymatic and systemic repercussions.</p>