AUTHOR=Li Xiaoya , Qiao Bo , Wu Yueying , Deng Na , Yuan Jiali , Tan Zhoujin 

TITLE=Sishen Pill inhibits intestinal inflammation in diarrhea mice via regulating kidney-intestinal bacteria-metabolic pathway

JOURNAL=Frontiers in Pharmacology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1360589

DOI=10.3389/fphar.2024.1360589

ISSN=1663-9812

ABSTRACT=<sec><title>Background</title><p>Sishen Pill (SSP) has good efficacy in diarrhea with deficiency kidney-yang syndrome (DKYS), but the mechanism of efficacy involving intestinal microecology has not been elucidated.</p></sec><sec><title>Objective</title><p>This study investigated the mechanism of SSP in regulating intestinal microecology in diarrhea with DKYS.</p></sec><sec><title>Methods</title><p>Adenine combined with <italic>Folium sennae</italic> was used to construct a mouse model of diarrhea with DKYS and administered with SSP. The behavioral changes and characteristics of gut content microbiota and short-chain fatty acids (SCFAs) of mice were analyzed to explore the potential association between the characteristic bacteria, SCFAs, intestinal inflammatory and kidney function-related indicators.</p></sec><sec><title>Results</title><p>After SSP intervention, the body weight and anal temperature of diarrhea with DKYS gradually recovered and approached the normal level. <italic>Lactobacillus johnsonii</italic> was significantly enriched, and propionic, butyric, isobutyric and isovaleric acids were elevated. Serum creatinine (Cr), interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α) levels of the mice were reduced, while serum blood urea nitrogen (BUN) and secretory immunoglobulin A (sIgA) in the colonic tissues were increased. Moreover, there were correlations between <italic>L. johnsonii</italic>, SCFAs, intestinal inflammatory, and kidney function.</p></sec><sec><title>Conclusion</title><p>SSP might suppress the intestinal inflammation by regulating the “<italic>L. johnsonii</italic>-propionic acid” pathway, thus achieving the effect of treating diarrhea with DKYS.</p></sec>