AUTHOR=Lin Shenghua , Ma Honglin , Zhang Shanshan , Fan Wei , Shen Chuanlin , Chen Jiayu , Jin Meng , Li Kun , He Qiuxia 

TITLE=The combination of paeonol, diosmetin-7-O-β-D-glucopyranoside, and 5-hydroxymethylfurfural from Trichosanthis pericarpium alleviates arachidonic acid-induced thrombosis in a zebrafish model

JOURNAL=Frontiers in Pharmacology

VOLUME=15

YEAR=2024

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2024.1332468

DOI=10.3389/fphar.2024.1332468

ISSN=1663-9812

ABSTRACT=<p><italic>Trichosanthis fruit</italic> (TF) is a classic medicinal material obtained from Shandong, China. The peel of this fruit (<italic>Trichosanthis pericarpium</italic>, TP) is known to exert anti-thrombotic effects. However, the anti-thrombotic active components and mechanisms of TP have yet to be fully elucidated. Combined with zebrafish models and high-performance liquid chromatography (HPLC), this study evaluated the endogenous anti-thrombotic effects with the combination of three compounds from TP. First, we used HPLC to investigate the components in the water extract of TP. Next, we used the zebrafish model to investigate the anti-thrombotic activity of the three compound combinations by evaluating a range of indicators. Finally, the expression of related genes was detected by real-time quantitative polymerase chain reaction (qPCR). HPLC detected a total of eight components in TP water extract, with high levels of paeonol (Pae), diosmetin-7-<italic>O</italic>-<italic>β</italic>-<italic>D</italic>-glucopyranoside (diosmetin-7-<italic>O</italic>-glucoside), and 5-hydroxymethylfurfural (5-HMF). The most significant anti-thrombotic activity was detected when the Pae: diosmetin-7-<italic>O</italic>-glucoside:5-HMF ratio was 4:3:3. qPCR analysis revealed that the abnormal expression levels of <italic>f2</italic>, <italic>fga</italic>, <italic>fgb</italic>, <italic>vwf</italic>, <italic>ptgs1</italic>, and <italic>tbxas1</italic> induced by arachidonic acid (AA) were improved. The combination of Pae, diosmetin-7-<italic>O</italic>-glucoside, and 5-HMF may alleviate AA-induced thrombosis by inhibiting the inflammatory reaction, coagulation cascade reaction, and arachidonic acid metabolism pathways.</p>