AUTHOR=Wang Feifei , Yu Bin , Yu Quanyong , Wang Guanglin , Li Baokun , Guo Ganlin , Wang Handong , Shen Hui , Li Shujin , Ma Chunling , Jia Xianxian , Wang Guiying , Cong Bin 

TITLE=NOP58 induction potentiates chemoresistance of colorectal cancer cells through aerobic glycolysis as evidenced by proteomics analysis

JOURNAL=Frontiers in Pharmacology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2023.1295422

DOI=10.3389/fphar.2023.1295422

ISSN=1663-9812

ABSTRACT=<p><bold>Introduction:</bold> The majority of individuals diagnosed with advanced colorectal cancer (CRC) will ultimately acquire resistance to 5-FU treatment. An increasing amount of evidence indicates that aerobic glycolysis performs a significant function in the progression and resistance of CRC. Nevertheless, the fundamental mechanisms remain to be fully understood.</p><p><bold>Methods:</bold> Proteomic analysis of 5-FU resistant CRC cells was implemented to identify and determine potential difference expression protein.</p><p><bold>Results:</bold> These proteins may exhibit resistance mechanisms that are potentially linked to the process of aerobic glycolysis. Herein, we found that nucleolar protein 58 (NOP58) has been overexpressed within two 5-FU resistant CRC cells, 116-5FuR and Lovo-5FuR. Meanwhile, the glycolysis rate of drug-resistant cancer cells has increased. NOP58 knockdown decreased glycolysis and enhanced the sensitivity of 116-5FuR and Lovo-5FuR cells to 5FU.</p><p><bold>Conclusion:</bold> The proteomic analysis of chemoresistance identifies a new target involved in the cellular adaption to 5-FU and therefore highlights a possible new therapeutic strategy to overcome this resistance.</p>