AUTHOR=Zhou Jing , Liu Jiang , Lin Qinqing , Shi Linhui , Zeng Zhigang , Guan Lichang , Ma Yunzi , Zeng Yingtong , Zhong Shilong , Xu Lishu 

TITLE=Characteristics of the gut microbiome and metabolic profile in elderly patients with sarcopenia

JOURNAL=Frontiers in Pharmacology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2023.1279448

DOI=10.3389/fphar.2023.1279448

ISSN=1663-9812

ABSTRACT=<p><bold>Introduction:</bold> There is growing evidence of research indicating that the gut microbiota is involved in the development of sarcopenia. Nevertheless, there exists a notable deficiency in comprehension concerning the connection between irregularities in the intestinal microbiome and metabolic processes in older individuals suffering from sarcopenia.</p><p><bold>Methods:</bold> To analyze fecal samples obtained from a cohort of 30 older patients diagnosed with sarcopenia as well as 30 older patients without sarcopenia, this study employed 16S rDNA sequencing and liquid chromatography-mass spectrometry (LC-MS)-based non-targeted metabolomics profiling techniques.</p><p><bold>Results:</bold> As a result, we found that 29 genera and 172 metabolites were significantly altered in the sarcopenic patients. Among them, <italic>Blautia</italic>, <italic>Lachnospiraceae_unclassified</italic>, and <italic>Subdoligranulum</italic> were the bacteria with a potential diagnostic value for sarcopenia diagnosis. Correlation analysis between clinical indices and these gut bacteria suggested that the IL-6 level was negatively correlated with <italic>Blautia</italic>. Function prediction analysis demonstrated that 17 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways differ significantly between sarcopenic and non-sarcopenic patients. The primary classes of metabolites identified in the study included lipids and lipid-like molecules, organic acids and derivatives, and organoheterocyclic compounds. KEGG enrichment analysis showed that purine metabolism, arginine and proline metabolism, alanine, aspartate, and glutamate metabolism, butanoate metabolism, and histidine metabolism may contribute to the development of sarcopenia. The correlation study on gut microbiota and metabolites found that <italic>Lachnospiraceae_unclassified</italic> was positively associated with seven metabolites that were more abundant in the non-sarcopenia group and negatively correlated with three metabolites that were more abundant in the sarcopenia group. In addition, <italic>Subdoligranulum</italic> was positively correlated with seven metabolites that were lacking in sarcopenia and negatively correlated with two metabolites that were enriching in sarcopenia. Moreover, <italic>Blautia</italic> was positively associated with xanthosine.</p><p><bold>Discussion:</bold> We conducted a study on the intestinal microbiota and metabolic profile of elderly individuals with sarcopenia, offering a comprehensive analysis of the overall ecosystem. Through this investigation, we were able to validate existing research on the gut–muscle axis and further investigate potential pathogenic processes and treatment options for sarcopenia.</p>