AUTHOR=Tran Thi Hoa My , Wang Rongbo , Kim Hoon , Kim Yeon-Ju 

TITLE=The anti-inflammation and skin-moisturizing effects of Boehmeria tricuspis-mediated biosynthesized gold nanoparticles in human keratinocytes

JOURNAL=Frontiers in Pharmacology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2023.1258057

DOI=10.3389/fphar.2023.1258057

ISSN=1663-9812

ABSTRACT=<p><bold>Introduction:</bold> Recently, nanotechnology has emerged as a potential technique for skin generation, which has several treatment advantages, such as decreased drug cytotoxicity and enhanced skin penetration. <italic>Boehmeria tricuspis</italic> (BT) belongs to the <italic>Urticaceae</italic> family and is rich in phenolic and flavonoid compounds. In this study, we biosynthesized gold nanoparticles (BT-AuNPs) using BT extract to explore their anti-inflammatory and skin-moisturizing properties in keratinocytes.</p><p><bold>Methods:</bold> Field-emission transmission electron microscopy, energydispersive X-ray spectrometry, dynamic light scattering, and Fourier-transforminfrared spectroscopy were used to examine the synthesized BT-AuNPs. qRT-PCR, western blot, and ELISA were applied for investigating the effect of BT-AuNPs on anti-inflammation and moisturizing activity in HaCaT cells.</p><p><bold>Results:</bold> At concentrations below 200 μg/mL, BT-AuNPs had no cytotoxic effect on keratinocytes. BT-AuNPs dramatically alleviated the expression and secretion of inflammatory chemokines/cytokine, such as <italic>IL-6</italic>, <italic>IL-8</italic>, <italic>TARC</italic>, <italic>CTACK</italic>, and <italic>RANTES</italic> in keratinocytes stimulated by tumor necrosis factor-<italic>α</italic>/interferon-<italic>γ</italic> (T + I). These anti-inflammatory properties of BT-AuNPs were regulated by inhibiting the NF-κB and MAPKs signaling pathways. Furthermore, BT-AuNPs greatly promoted hyaluronic acid (HA) production by enhancing the expression of hyaluronic acid synthase genes (<italic>HAS1</italic>, <italic>HAS2</italic>, and <italic>HAS3</italic>) and suppressing the expression of hyaluronidase genes (<italic>HYAL1</italic> and <italic>HYAL2</italic>) in HaCaT cells.</p><p><bold>Discussion:</bold> These results suggest that BT-AuNPs can be used as a promising therapeutic alternative for treating skin inflammation. Our findings provide a potential platform for the use of BT-AuNPs as candidates for treating inflammatory skin diseases and promoting skin health.</p>