AUTHOR=Xiu Yanghui , Su Yu , Gao Lihua , Yuan Hui , Xu Sennan , Liu Ying , Qiu Yan , Liu Zhen , Li Yuhang 

TITLE=Corylin accelerated wound healing through SIRT1 and PI3K/AKT signaling: a candidate remedy for chronic non-healing wounds

JOURNAL=Frontiers in Pharmacology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2023.1153810

DOI=10.3389/fphar.2023.1153810

ISSN=1663-9812

ABSTRACT=<p><bold>Introduction:</bold> Chronic non-healing wound is a considerable clinical challenge and research into the discovery of novel pro-healing agents is underway as existing therapeutic approaches cannot sufficiently meet current needs.</p><p><bold>Method:</bold> We studied the effects of corylin in cell line fibroblasts and macrophages by Western blots, PCR, Flow cytometry assay, Immunofluorescence.</p><p><bold>Results:</bold> We showed that corylin, a main flavonoid extracted from <italic>Psoralea corylifolia</italic> L, reduced inflammatory responses, promoted collagen deposition, and accelerated the healing of full-thickness skin wounds in mice. Exploration of the underlying mechanisms showed that corylin activated the PI3K/AKT signaling, leading to fibroblasts’ migration, proliferation, and scratch healing. Corylin also activated sirtuin 1 (SIRT1) signaling, enhanced the deacetylation and cytoplasmic translocation of NF-κB p65, and therefore reduced lipopolysaccharide (LPS)-induced inflammatory responses in macrophages. Furthermore, inhibition of PI3K/AKT and sirtuin 1 pathway with LY294002 and EX527 prevent the therapeutic potency of corylin against chronic wounds.</p><p><bold>Conclusion:</bold> In summary, our results suggested that corylin may be a candidate for the development of novel pro-healing agents.</p>