AUTHOR=Dong Zhenghan , Wang Yachao , Hao Cuiting , Cheng Yuan , Guo Xi , He Yanyu , Shi Yueyue , Wang Shuang , Li Yunqi , Shi Wei 

TITLE=Sanghuangporus sanghuang extract extended the lifespan and healthspan of Caenorhabditis elegans via DAF-16/SIR-2.1

JOURNAL=Frontiers in Pharmacology

VOLUME=14

YEAR=2023

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2023.1136897

DOI=10.3389/fphar.2023.1136897

ISSN=1663-9812

ABSTRACT=<p><italic>Sanghuangporus Sanghuang</italic> is a fungus species. As a traditional Chinese medicine, it is known for antitumor, antioxidant and anti-inflammatory properties. However, the antiaging effect of <italic>S. Sanghuang</italic> has not been deeply studied. In this study, the effects of <italic>S. Sanghuang</italic> extract (SSE) supernatants on the changes of nematode indicators were investigated. The results showed that different concentrations of SSE prolonged the lifespans of nematodes and substantially increased these by 26.41%. In addition, accumulations of lipofuscin were also visibly reduced. The treatment using SSE also played a role in increasing stress resistance, decreasing ROS accumulations and obesity, and enhancing the physique. RT–PCR analysis showed that the SSE treatment upregulated the transcription of <italic>daf-16</italic>, <italic>sir-2.1</italic>, <italic>daf-2</italic>, <italic>sod-3</italic> and <italic>hsp-16.2</italic>, increased the expression of these genes in the insulin/IGF-1 signalling pathway and prolonged the lifespans of nematodes. This study reveals the new role of <italic>S. Sanghuang</italic> in promoting longevity and inhibiting stress and provides a theoretical basis for the application of <italic>S. Sanghuang</italic> in anti-ageing treatments.</p>