Using omics approaches to dissect the therapeutic effects of Chinese herbal medicines on gastrointestinal cancers

Chinese herbal medicines offer a rich source of anti-cancer drugs. Differences between the pharmacology of Chinese herbal medicines and modern synthetic chemicals hinder the development of drugs derived from herbal products. To address this challenge, novel omics approaches including transcriptomics, proteomics, genomics, metabolomics, and microbiomics have been applied to dissect the pharmacological benefits of Chinese herbal medicines in cancer treatments. Numerous Chinese herbal medicines have shown potential anti-tumor effects on different gastrointestinal (GI) cancers while eliminating the side effects associated with conventional cancer therapies. The present study aimed to provide an overview of recent research focusing on Chinese herbal medicines in GI cancer treatment, based on omics approaches. This review also illustrates the potential utility of omics approaches in herbal-derived drug discovery. Omics approaches can precisely and efficiently reveal the key molecular targets and intracellular interaction networks of Chinese herbal medicines in GI cancer treatment. This study summarizes the application of different omics-based approaches in investigating the effects and mechanisms of Chinese herbal medicines in GI cancers. Future research directions are also proposed for this area of study.

Introduction

Gastrointestinal (GI) cancer refers to malignant conditions of the gastrointestinal system and encompasses colorectal, gastric, hepatocellular, pancreatic, cholangiocarcinoma, and esophageal cancers (Torre and Bray et al., 2015; Abdelfatah et al., 2016). Most patients with GI cancers are diagnosed at advanced stages due to the limitations of diagnostic approaches and conventional therapy (Rahbari et al., 2016). It is of paramount importance to improve the early diagnosis and treatment efficacy, thereby reducing the mortality arising from GI cancers. For proper diagnosis and treatment of GI cancers, it is imperative to develop effective screening methods capable of detecting precancerous lesions and revealing the pathological and molecular mechanisms of GI cancers.

TABLE 1

TABLE 1. Applications of genomics approaches for the testing of Chinese herbal medicines in GI cancers.

TABLE 2

TABLE 2. Applications of proteomics approaches to study the mechanisms of Chinese herbal medicines in GI cancers.

TABLE 3

TABLE 3. Application of metabolomics approaches to study Chinese herbal medicines in GI cancers.

TABLE 4

TABLE 4. Applications of transcriptomics approaches to study Chinese herbal medicines in GI.

TABLE 5

TABLE 5. Applications of microbiomics approaches to study Chinese herbal medicines in GI cancers.

The omics field is a combination of high-throughput and specialized biotechnological assays, equipment, and algorithms comprising transcriptomics, genomics, interactomics, proteomics, phenomics, and metabolomics. Omics disciplines can also include subdisciplines, which require further specialization in computational and analytical methods (Pirih and Kunej, 2017). For genomics analyses, technical approaches mainly include RNA transfection, microRNA (miRNA) transfection, and real-time PCR analysis. Transcriptomics analyses consist of RNA sequencing, DNA microarray, and expressed sequence tag (EST) technology. Proteome profiler antibody arrays and SWATH-MS are common methods used in proteomics while IN NMR, LC-MS, and UPLC-Q-TOF/MS are used for metabolomic analyses. As for microbiomics, high-throughput sequencing and mass spectrometry are common approaches. To date, novel experimental methods, including high-throughput technologies, have been utilized for collecting large medical omics datasets, which have proven to be invaluable for oncology research.

Natural products with medicinal components possess potential pharmaceutical benefits in the treatment of cancer. Chinese herbal medicines that can be used to treat GI cancers include three categories; botanical drugs, Chinese herbal formulations, and herbal medicine compounds. For example, the botanicals Scleromitrion diffusum and Hericium erinaceus are commonly utilized to treat gastrointestinal diseases, including gastric cancer; they exert their anti-cancer actions by inhibiting tumor angiogenesis, proliferation, and promoting apoptosis [Lu et al., 2019 (PMID: 32265701); Li F. et al., 2015 (PMID: 24631140)]. Chinese herbal medicines have been shown to improve the efficacy of conventional GI cancer treatment, alleviate adverse effects, and reverse drug resistance (Hu et al., 2016). In addition, traditional Chinese medicine (TCM) syndrome is a patient-centered clinical manifestation profile, which contributes to the individualized treatment of cancer (Ji et al., 2016). The characteristics and advantages of TCM in cancer treatment are based on syndrome differentiation [Jiang et al., 2020 (PMID: 22322251)]. Combined with syndrome differentiation, the application of omics has great clinical significance in TCM treatment of GI cancers (Zhang and Shang, 2011). However, systematic summaries on how omics assist in understanding the mechanisms of Chinese herbal medicines in GI cancer treatment remain scarce.

This study provides a comprehensive review of the latest omics approaches used for characterizing the anti-tumor actions of Chinese herbal medicines on GI cancers and their ability to attenuate adverse effects arising from conventional cancer therapy. We generalized several omics methods that reveal the potential molecular mechanisms of anti-tumor Chinese herbal medicines in intracellular signaling pathway networks, key targets, and host gut microbiota (Figure 1). Omics data can be subsequently analyzed and validated by systems biology, network pharmacology, in vitro and in vivo experiments, and ultimately in clinical trials. In addition, we also discuss existing limitations and prospects of omics approaches in this field.

FIGURE 1

FIGURE 1. Application of omics assays in studying Chinese herbal medicines for treating gastrointestinal cancer. Application of omics approaches in GI cancer treatment with Chinese herbal medicines.

Most clinical GI cancer cases include gastric cancer, colorectal cancer, hepatocellular carcinoma (HCC), esophageal cancer, pancreatic cancer, and cholangiocarcinoma (Torre et al., 2015; Abdelfatah et al., 2016). Omics approaches are widely utilized to explore the efficacy and molecular mechanisms of Chinese herbal medicines in treating GI cancers. Despite the reproducibility, noise disturbance, and complexity of omics, the utilization of omics approaches can improve the likelihood of identifying active anti-tumor ingredients from herbal medicines. Moreover, omics approaches can be used to further dissect the molecular mechanisms underlying the anti-tumor activity of Chinese herbal medicines, such as inducing apoptotic cell death, relieving oxidative damage, and regulating host immunity [Wang et al., 2014 (PMID: 25379508); Oyenihi et al., 2021 (PMID: 34770949)].

Genomics

The field of genomics was initially used for DNA sequencing, from which it has rapidly expanded to more widely encompassing functional assessments—exploring the roles of both proteins and genes as well as their associated expression profiles. The emergence of novel herbal genomics research, together with advances in other omics approaches may improve the discovery of novel anti-GI agents arising from Chinese herbal medicines.

A previous study utilized genomics analysis to identify that PepE, a natural secolignan isolated from the Chinese botanical herb Peperomia dindygulensis, may prevent gastric cancer metastasis and inhibit DNA methylation in epigenetic cancer therapy (Wanga et al., 2018). RNA sequencing and miRNA sequencing found that berberine (BBR), isolated from Coptis chinensis, could inhibit the proliferation of SGC-7901 gastric cancer cells and induce cell apoptosis (Yang et al., 2018). In addition, through microarray experiments, ursolic acid, which exists in the whole grass of Prunella vulgaris L., the leaves of Ilex rotunda Thunb, and many other botanical drugs, was found to exhibit inhibitory effects on the metastasis and proliferation of gastric cancer cells by modulating the Hippo pathway via the tumor suppressor gene Rassf1 (Kim et al., 2019). Periplocin, an extract from the traditional herbal medicine cortex periplocae (Periploca sepium Bunge), may inhibit the proliferation of gastric cancer cells via the ERK1/2-EGR1 pathway (Li et al., 2016). Furthermore, a recent study used high-throughput RNA sequencing, real-time PCR, gene ontology (GO) enrichment, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, to show that p-coumaric acid, a phenolic ingredient isolated from various Chinese herbal plants, exhibits remarkable anti-tumor effects against the gastric cancer cell line SNU-16 by regulating miRNA expression (Jang et al., 2020). Furthermore, cetuximab and β-elemene, bioactive compounds isolated from the Chinese botanical drug Curcumae Rhizoma, can inhibit tumor growth and lymph node metastases in mice with colorectal cancer (Chen et al., 2020). Tupistra chinensis Baker (Rohdea chinensis), an anti-tumor Chinese botanical drug, inhibits the growth of gastric cancer in vivo (Wang et al., 2021). Ying et al. (2018) systematically illustrated the therapeutic potential of botanical drugs for esophageal cancer treatment in vitro and in vivo. The aforementioned studies indicate that Chinese herbal medicines and their active compounds may offer potential therapeutic value for the treatment of GI cancers by regulating cell apoptosis, proliferation, and metastasis.

Hepatocellular carcinoma (HCC) is an increasingly prevalent difficult-to-manage malignant liver disease. Omics approaches may reveal the anti-HCC mechanisms of various Chinese herbal medicines, such as inducing apoptotic cell death, autophagy, and regulating various non-coding RNAs (Dai et al., 2022). However, very few reports have utilized genomics experiments to investigate the anti-HCC effects of Chinese herbal medicines. In this field, cDNA microarrays comprise the most widely used genomics technology. A recent study that utilized a cDNA microarray assay found that curcumin could regulate various kinase gene expressions in HCC. Among the 79 kinase genes, the expressions of 31 genes were significantly increased while 29 genes were decreased, suggesting that curcumin might inhibit HCC cell proliferation by targeting the protein kinase C pathway (Kao et al., 2011). Using cDNA microarray hybridization, the Perilla frutescens extract (PLE) was shown to induce apoptosis by regulating the expression of numerous apoptosis-related genes in HepG2 cells (Lin et al., 2007). Although the aforementioned studies highlight the pivotal role of genomics approaches in discovering novel anti-HCC agents, there remain a considerable number of Chinese herbal medicines that await genomic investigations.

Several studies have shown that genomics could help characterize the anti-cancer mechanisms of numerous Chinese herbal medicines for esophageal cancer. For example, microarray quantification and real-time PCR showed that treatment with Andrographis paniculate (AP) resulted in decreased expression of AXIN2, WNT4, RTKN2, EGFR, OLFML1, BMP4, PDGFRB, SMAD7, MYH10, ABCA13, and ABCB1, while ZNF704 and ASNS were upregulated in esophageal cancer cells. These results indicated that several intracellular signaling processes associated with cell apoptosis, proliferation, metastatic processes, intercellular adhesion, and drug resistance were remarkably modulated after AP treatment.

A novel derivative of ajoene, BisPMB can be isolated from the Chinese herbal medicine garlic. In a recent study, a DNA microarray assay was applied in combination with gene ontology, KEGG, and an ingenuity pathway analysis to explore the active mechanisms of BisPMB in esophageal cancer. The results showed that BisPMB can inhibit esophageal cancer cell proliferation through the unfolded protein response activation via the GADD153/CHOP pathway (Siyo et al., 2017). Moreover, several studies have shown that microarray analysis could help reveal the underlying mechanism of Chinese herbal medicines in regulating esophageal cancer cell metastasis. Sulforaphene (SFE), a novel isothiocyanate in radish seeds, has been found to inhibit esophageal cancer cell metastasis and proliferation. Upon treating the esophageal cancer EC109 cell line with SFE, microarray analysis showed significant downregulation of the metastasis-related CDH3 and SCD mRNA, while p38 activators such as GADD45B and MAP2K3 were upregulated (Han et al., 2020). In addition, RNA sequencing technologies show that SQSTM1/p62 (autophagy markers) was upregulated in esophageal cancer cells after treatment with phlorizin, the main component of the sweet tea leaf (Lithocarpus polystachyus Rehd). These data suggest that phlorizin can inhibit autophagy in esophageal cancer by regulating the ubiquitin-binding protein SQSTM1/P62 (Jia et al., 2021).

In pancreatic cancer, microarray assays are the most frequently applied genomics technology for exploring potential anti-cancer mechanisms of Chinese herbal medicines. For example, by using microarray assays, Fv1, an extract from Fucus vesiculosus was shown to regulate the cell cycle inhibitor p57, thereby leading to cell cycle arrest in pancreatic cancer cells (Geisen et al., 2015). Another study found that paeoniflorin could reduce cell proliferation and induce apoptosis in pancreatic cancer cells. Through microarray analysis, HTRA3 was significantly increased in paeoniflorin-treated cells, further inhibiting cell proliferation and inducing cell apoptosis (Li et al., 2017). Green tea, made from Camellia sinensis leaves, has been used as both a beverage and medicine in traditional Chinese culture. A recent study used microarrays to show that tea extracts offer an effective preventative for the tumorigenesis of pancreatic cancer cells; the downregulation of ID1, which is highly expressed in cancer cells, may be related to these inhibitory effects (Zheng et al., 2019).

Proteomics

Proteomics assays include shotgun proteomic analysis, two-dimensional liquid chromatography–tandem mass spectrometry (2D-LC–MSMS), isobaric tags for relative and absolute quantification (iTRAQ), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and two-dimensional difference gel electrophoresis. The application of proteomics assays has offered preliminary biological evidence for the potential utility of Chinese herbal medicines in treating gastric cancer. For example, Tan et al. (2019)utilized a shotgun proteomic analysis and found that cytochrome c, an active component purified from Aspongopus chinensis, might be a noteworthy anti-cancer agent for gastric cancer treatment. The Weining granule (WNG), a Chinese medicine formula that comprises Poria cocos (Chinese name: Fuling, FL), Astragalus mongholicus Bunge (Chinese name: Huangqi, HQ), Fructus Lycii (Chinese name: Gouqizi, GQZ), Curcumae rhizoma (Chinese name: Ezhu, EZ), Solanum nigrum (Chinese name: Longkui, LK), Ranunculus ternatus Thunb. (Chinese name: Maozhuacao, MZC), and Rhizoma Paridis (Chinese name: Chonglou, CL) has been widely used for treatment of GI patients. Liang et al. (2021)showed that WNG treatment altered the abundance of 192 proteins in gastric cancer cells. These proteins were associated with carbon metabolism, cholesterol metabolism, alanine, glutamate and aspartate metabolism, and TNF signaling pathways in gastric cancer.

In HCC, the mechanisms of action for Chinese herbal medicines have been characterized using multiple proteomics approaches. Previous studies have shown that reduced actin-binding protein profilin 1 (PFN1) might be associated with HCC and can indicate a poor prognosis. Based on proteomic analyses, guttiferone K (GUTK) isolated from the botanical drug genus Garcinia, was shown to inhibit HCC cell invasion and migration by regulating PFN1 expression (Shen et al., 2016). Platycodin D (PD) is a triterpenoid saponin extracted from Platycodon grandiflorus. After PD-treatment in HCC cells, 16 proteins were increased and three proteins were decreased, as determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and two-dimensional difference gel electrophoresis (Lu et al., 2015). Recently, a study using 2D-LC–MSMS combined with an iTRAQ assay identified 198 and 113 differentially expressed proteins after Viscum coloratum polysaccharide 2 (VCP2) treatment in HCC and normal hepatic cells, respectively. These findings broaden our understanding of the anti-tumor mechanisms of the active ingredients in Viscum coloratum and offer novel assays for screening proteins, which comprise potential targets of polysaccharides (Chai and Zhao, 2017). In addition, a reversed-phase proteomic array (RPPA) analysis combined with proteomics found that fraxini, a mistletoe extract, suppresses the proliferation of HCC by decreasing the expression of c-Myc [Yang et al., 2019 (PMID: 31015523)]. In 2019, Hou et al. (2020) used proteomic techniques to elucidate the anti-HCC mechanism of dihydroartemisinin, a semi-synthetic derivative of artemisinin. They showed that dihydroartemisinin can up-regulate the APOA1 protein and downregulate GALNT10. Huacheng et al. analyzed the protein expression profiles of human HCC cells after treatment with Gexia Zhuyu Decoction (GXZY). Protein electrophoretic profiles of the treatment and control groups were analyzed by mass spectrometry, and six metastasis-related proteins were identified, suggesting that GXZY drug-containing sera may alter protein expression in human HCC cells (Hua-cheng and Jian-gang, 2015).

In colon cancer, proteomics assays have also been extensively used to interrogate the underlying mechanisms of Chinese herbal medicines. Xiong et al. (2020)explored the anti-tumor efficacy of AUCAN, a type of dibenzofuran, in vitro and in vivo colorectal cancer (CRC) models. Further mechanistic studies using proteomic and functional clustering analyses showed that AUCAN treatment upregulated 24 proteins and downregulated 42 proteins, suggesting that AUCAN treatment may regulate multiple genes in CRC. Chen et al. (2021)conducted a label-free based quantitative proteomic analysis to evaluate the protein expression profiles in colon cancer cells treated with the traditional Chinese medicine Camellia nitidissima Chi (CNC). They showed that CNC regulated CRC cell proliferation via regulation of ferroptosis signaling. The presented evidence indicates that proteomics analyses can be used to interrogate the mechanisms of Chinese herbal medicines in GI cancer treatment.

Metabolomics

Metabolomics approaches primarily consist of ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS), metabolic profiling, UPL−ESI−QTOFMS, 1H-NMR spectroscopy, and GC–MS combined with multivariate statistical analyses. Previously, metabolomics was used to explore the anti-gastric cancer mechanisms of Chinese herbal medicines. Several studies have utilized proteomics in the study of Chinese herbal medicine in HCC and colon cancer. Metabolomics has the potential to reveal the active anti-cancer components present in Chinese herbal medicines.

Based on 1H-NMR spectroscopy, Gnocchi et al. (2021)found that the ethyl acetate extract of Crithmum maritimum can reverse the Warburg effect (preferential use of glycolysis rather than oxidative phosphorylation for energy production by tumor cells) in HCC cells by decreasing intracellular lactate. The ethyl acetate extract can also inhibit protein anabolism by reducing the intracellular level of amino acids and alter membrane biosynthesis by lowering choline and phosphocholine contents in HCC. Furthermore, Kim et al. (2015) applied a GC–MS assay coupled with multivariate statistical analysis and described the multitarget action of the ethyl acetate extract of Crithmum maritimum in suppressing HCC cell proliferation. A metabolomics investigation utilizing LC–MS tested the active components of toad venom that show potential anti-tumor mechanisms in HCC. They showed that the combination of toad venom and a Chinese toad-shortening base could cause HCC tumor metabolic disorders, and reduce mitochondrial membrane potential, relieve oxidative damage of cancer cells, and inhibit energy metabolism. Metabolomics analysis found that terpenoids, flavonoids, alkaloids, phenylpropanoids, and fatty acids are the most abundant bioactive components of Astragalus membranaceus, with anti-tumor, anti-oxidation, and anti-inflammation effects (Wu et al., 2020). Based on UPLC–MS-based metabolomics approaches, betulinic acid was identified as the most active anti-cancer compound in Forsythiae Fructus (Bao et al., 2018).

In colon cancer, metabolomics have revealed the mechanisms underlying novel cancer treatments using Chinese herbal medicines. Yang et al. used an UPLC–MS/MS assay to identify that shikonin could inhibit CRC cell growth via various signaling pathways that included glutathione metabolism, arginine biosynthesis, purine metabolism, beta-alanine metabolism, and arginine biosynthesis (Chen et al., 2020b). Daddiouaissa et al. (2021)performed metabolic profiling and utilized multivariate data analysis combined with an ingenuity pathway analysis (IPA) to explore the effects of treating CRC cells with the ionic liquid-Graviola fruit pulp extract (IL-GPE). A pathway analysis of metabolomic profiles showed significant alterations in pathways related to cancer cell growth and energy metabolism, including aerobic glycolysis, amino acid metabolism, urea cycle, and ketone body metabolism [Pan et al., 2021 (PMID: 34547237)]. The authors concluded that IL-GPE might be useful for the treatment of CRC via selectively suppressing cancer cell proliferation and energy metabolism (Daddiouaissa et al., 2021). Li et al. adopted an UPL−ESI−QTOFMS assay to examine the metabolic signatures of CRC cells after nutmeg treatment, demonstrating inhibition of CRC development due to alleviation of dysregulated lipid metabolism and normalization of gut microbial metabolism (Li W. B. et al., 2015). Dysfunction of the gut microbiome contributes to the development of GI cancers [Peng et al., 2020 (PMID: 32855157); Song et al., 2020 (PMID: 31586566)]. Chinese herbal medicines have shown considerable utility in the treatment of metabolic disorders via their regulation of the gut microbiota. Zhang et al. reported that Chinese herbal medicines can affect glucose and lipid metabolism by modulating the gut microbiota, including anti-inflammatory bacteria, lipopolysaccharide- and short-chain fatty acid-producing bacteria, and bacteria with bile-salt hydrolase activity [Zhang et al., 2020 (PMID: 33197760)]. Based on metabolomics approaches, Chinese herbal medicines might provide a promising complementary therapy for CRC patients.

Transcriptomics

Transcriptomics comprises high-throughput assays to assemble a complete set of RNA transcripts in a specific cell or culture of cells under specific conditions. Because of their accuracy, sensitivity, high throughput, reproducibility, and specificity, DNA microarrays have been widely applied to study the transcriptomics of Chinese herbal medicine. In addition, RNA sequencing (RNA-seq) is also widely used and can be applied to the analysis of the therapeutic mechanisms of Chinese herbal medicines [Chambers et al., 2018 (PMID: 30264869); Duan et al., 2021 (PMID: 34074345)]. Therefore, in this review, we focus on an overview of transcriptomics assays in GI cancer treatment using Chinese herbal medicines.

Tanshinone IIA (TIIA) is a diterpene quinone extract isolated from the Chinese botanical drug Salvia miltiorrhiza. In gastric cancer, the TIIA anti-tumor effects and mechanisms were analyzed by RNA-seq transcriptomics combined with iTRAQ. TIIA significantly inhibited the gastric cancer cell line AGS by controlling the glucose metabolism of cancer cells (Lin et al., 2015).

Transcriptomic analysis can interrogate various nucleic acids including micro RNAs (miRNA), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs), to explain the therapeutic effects of herbal medicines on cancers. Lan et al. (2021)investigated the transcriptome-level mechanisms of baicalin-mediated anti-tumor effects on osteosarcoma (OS) and identified 58 lncRNAs and 31 miRNAs that responded to the treatment. In addition, baicalin can suppress the malignant phenotypes of CRC by regulating the circRNA MYH89/miR-761 axis (Zhang et al., 2021). Based on high-throughput sequencing, Cheng et al. (2021) found that the herbal ingredient apigenin (API) suppressed CRC cell proliferation by decreasing E2F1/3 and regulating miRNA-215-5p. However, to our knowledge, the potential involvement of GI cancer circRNA modulation by Chinese herbal medicines has not been interrogated.

Based on transcriptome profiling of colon cancer cells after treatment with Thalassia testudinum extracts (TTE), a recent study found that TTE can trigger multiple stress signaling pathways (endoplasmic reticulum stress, unfolded protein stress, DNA damage, and nitrosative stress) that induced CRC cell apoptosis. This evidence supports the potential use of TTE in CRC treatment (Hernandez-Balmaseda and Guerra et al., 2021). Shikonin is a commonly used naphthoquinone ingredient isolated from the Chinese botanical drug Lithospermum erythrorhizon. . Chen et al. (2020c) combined metabolomics and transcriptomics to assess the anti-cancer activity of shikonin in human CRC cells. They showed that shikonin exhibits significant anti-tumor activity and that the regulation of purine metabolism may contribute to the anti-cancer effects of shikonin.

Microbiomics

Both environmental and host factors can contribute to the development of GI cancers. The intestinal microbiota (IM) can directly affect health and play a pivotal role in GI cancer development. In recent years, with the rapid development of high-throughput sequencing technology, the relationship between the IM and their metabolites, and the development, diagnosis, and treatment of CRC have been elucidated. Herein, we review previous work utilizing microbiomics to explore the potential mechanisms of Chinese herbal medicine extracts for the treatment of gut microbiota-related GI cancers.

Curcumin, derived from the rhizome of the Curcuma longa plant, was found to function as an analgesic, antiseptic, antioxidant, anti-inflammatory, chemo-sensitizing, chemo-preventive, and radio-sensitizing agent on colon tumorigenesis (McFadden et al., 2015). This work was completed using a 16S amplicon library, miSeq sequencing, and quantitative insights into microbial ecology (QIIME) analysis of the gut. The role of Chinese ginseng in modulating the intestinal microbiome for colon cancer prevention has also been explored through microbiomics. It was suggested that the CRC chemo-preventive effects of Chinese ginseng are mediated through enteric microbiome population-shift recovery and dysbiosis restoration. Furthermore, ginseng regulation of microbiome balance contributes to the maintenance of enteric homeostasis (Wang et al., 2018). The effects of extracts from Chinese herbal medicines in treating colitis-associated carcinogenesis have been explored using 16S rRNA gene sequence analysis and bioinformatic analysis of the gut microbiota via examinations of fecal samples. Bound polyphenol of inner shell (BPIS) is extracted from foxtail millet bran, and was shown to remodel the overall gut microbiota composition toward normal in tumor-bearing mice; this included two phyla and eight genera, together with modulations of various genes that regulate 17 signal pathways in colon cancer (Yang et al., 2020). Sui et al. (2020)demonstrated the inhibitory effects of the traditional Chinese botanical drug (Yi-Yi-Fu-Zi-Bai-Jiang-San) on colon carcinogenesis by examining gut bacterial diversity and performing human-to-mouse fecal microbiota transplantation experiments. Jiang et al. (2020)utilized 16S rDNA sequencing of intestinal flora and discovered that Wu Mei Wan, a nine-ingredient Chinese Medicine formula, can attenuate colitis-associated CRC by balancing tumor-promoting and tumor-suppressing bacteria. Alisol B 23-acetate, a natural tetracyclic triterpenoid found in Alismatis rhizome, also possesses therapeutic effects on colitis-associated cancers via a reduction of pathogenic bacteria, such as Klebsiella, Citrobacter, and Akkermansia (Zhu et al., 2021).

Omics facilitates the understanding of mechanisms of Chinese herbal medicines in treating CI cancers and alleviating the adverse effects of conventional therapies

Chinese herbal medicines can effectively alleviate or even eradicate the adverse effects of conventional tumor treatments. Pain, bloating, nausea, vomiting, and diarrhea are common adverse effects of radiation exposure or chemotherapy in patients with GI cancers. Based on omics approaches, we can characterize the potential mechanisms of Chinese herbal medicines in alleviating these adverse effects. The therapeutic effects and mechanisms of Chinese herbal medicines on GI cancers can be explored by genomics analysis. For example, DNA microarray was applied to analyze the gene expression profiles of HCC cells treated with San-Huang-Xie-Xin-Tang (SHXXT), thereby obtaining the therapeutic mechanisms of SHXXT on HCC [Lin et al., 1994 (PMID: 7898124)]. Metabolomics is an analytical profiling technique for measuring and comparing metabolites in biological samples. Combining high-throughput analytical chemistry and multivariate data analysis, metabolomics provides an important foundation for metabolic mechanisms of Chinese herbal medicines treating GI cancers. A recent study revealed the potential mechanisms of the anti-inflammatory effects of Glycyrrhiza extracts in chemotherapy-induced colitis via a plasma metabolomics approach. Glycyrrhiza Linn., also known as licorice, is a commonly used Chinese herbal medicine. Glycyrrhiza contains multiple flavonoids and exhibits significant anti-inflammatory effects both in vitro and in vivo [Leite et al., 2022 (PMID: 35456938)]. Eleven differential endogenous metabolites associated with the anti-inflammatory effects of licorice flavonoids were shown, such as linoleic acid, sphingosine, corticosterone, leukotriene B4, and tryptophanamide. The multi-pathway-integrated mechanisms of licorice flavonoid action may alleviate chemotherapy-induced colitis in GI patients, which can improve the development of novel complementary agents for reducing the adverse effects of conventional GI treatment (Yang et al., 2020). Previous studies have suggested that various chemotherapy agents must be weighed against possible adverse effects on the hepatic tissue in patients with GI cancers [You et al., 2017 (PMID: 28458594); Wang et al., 2020 (PMID: 18458704)]. For patients with pre-existing hepatic disorders, conventional cancer treatment carries a significant risk of hepatotoxicity which can lead to hepatitis, bleeding, or cholestasis. A recent study found that injection with the compound kushen can effectively rebalance the Smad7/TGF-β signaling pathway in HSCs and alleviate chemotherapy-induced hepatotoxicity in patients with GI cancers (Yang et al., 2020). In addition, oral mucositis (OM), which can interrupt conventional cancer treatment, can affect the control of the disease and quality of life in patients. Transcriptomics and metabolomics assays showed that the Chinese herbal Shuanghua Baihe tablet (SBT) can alleviate chemotherapy-induced OM in colon cancer patients by regulating linoleic acid metabolism, glycerophospholipid metabolism, and amino acid metabolism, as well as by inhibiting IL-17/TNF signal transduction to restore Treg and Th17 cell homeostasis in OM rats (Geng et al., 2021).

Based on these data, we offer that different omics can be jointly applied to explore the mechanisms of Chinese herbal medicines in treating GI cancers. Genomics, transcriptomics, and proteomics analyze the biological functions and molecular mechanisms at the DNA, RNA, and protein levels, respectively. Metabolomics can be used to quantify small molecules and metabolites in cells and biological systems. It provides a measure of the inputs and outputs of biological pathways and is considered to be particularly powerful in describing the functional cell state, in comparison to other omics approaches such as genomics and proteomics. In addition, microbiomics collectively characterizes and quantifies molecules responsible for the structure, function, and dynamics of a microbial community, contributing to understanding microbial behaviors under different environmental conditions and providing a more suitable environment for disease treatment. To properly and comprehensively understand the mechanisms of Chinese herbal medicines underlying their effects on GI cancers, all omics can be used to analyze cancer cells and tissues after treatment with Chinese herbal medicines. As a result, data that describe the biological functions, molecular mechanisms, and microbial alterations can be collected more comprehensively, thereby facilitating the development of more appropriate therapeutic strategies and clinical decision making. The application of multi-omics approaches can also be used to reduce adverse effects. A comprehensive application of all omics approaches enriches our understanding of the biological functions, molecular mechanism, and microbial alterations that take place in response to Chinese herbal medicine treatment of GI cancers.

Conclusion and future prospects

Active components extracted from Chinese herbal medicines and their active ingredients are highly effective for treating GI malignancies. For example, berberine represses human gastric cell growth by inducing cytostatic autophagy (Zhang et al., 2020). In addition, Jianpi Yangzheng Xiaozheng Decoction can prevent the progress of gastric cancer and inhibit the gastric cancer epithelial–mesenchymal transition (Wu et al., 2019). Betulinic acid delays tumor growth and inhibits pulmonary metastasis (Chen et al., 2020b). Curcumin can suppress cell proliferation and induce apoptosis in gastric cancer cells (Sun et al., 2019). Yiqi Jianpi Huaji Decoction can inhibit gastric cancer cell proliferation, induce apoptosis, and increase sensitivity to chemotherapeutic agents by decreasing the expression of TUBB3, MRP, P-gp, and STMN1 (Li F. et al., 2015). Wang et al. (2020) showed, in clinical studies, that Banxia XieXin Decoction treatment is safe and effective for patients with advanced HCC). Moreover, in a study by Zhang et al. (2017), combined astragaloside IV and curcumin therapy inhibited tumor growth and angiogenesis in an orthotopic nude-mouse model of human hepatocellular carcinoma (Zhang et al., 2017). Baicalin, the major active ingredient of the Chinese herbal medicine Scutellaria baicalensis, induces colon cancer cell apoptosis by inhibiting miRNAs (Tao et al., 2018). In terms of pancreatic cancer, Astragalus polysaccharide enhanced the anti-tumor effects of apatinib by downregulating the phosphorylation of AKT, ERK, and MMP-9 (Wu et al., 2018). However, many Chinese herbal medicines have not been approved in clinical trials because of their incompletely characterized active components and molecular mechanisms. Thus, the application of various omics assays may improve the rapid development of novel anti-cancer agents from Chinese herbal medicines. In the current review, the aforementioned studies have indicated that multiple omics approaches can assist in validating potential therapeutic drugs and promote the understanding of the underlying mechanisms of Chinese herbal medicines in GI cancer treatment.

Genomics, proteomics, metabolomics, transcriptomics, and microbiomics assays have been widely applied to analyze the role of Chinese herbal medicines in GI cancer treatment and its molecular mechanisms (Figure 2). However, many implementational limitations still exist. First, the complex ingredient list of Chinese herbal medicines hampers the process of characterizing bioactive ingredients and the initial investigation of their systemic actions. Second, we have not yet been able to deliver a comprehensive understanding of the toxicological effects and pharmacological activities associated with Chinese herbal medicines. Finally, the current chemical databases and laboratory equipment do not allow scientists to research as many of the chemical ingredients as possible, which poses further challenges when characterizing the mechanism as a whole.

FIGURE 2

FIGURE 2. Underlying molecular mechanisms of Chinese herbal medicines in GI cancer treatment revealed by omics approaches.

In general, we suggest that additional bioinformatics analyses are integrated with omics assays to explore and exploit the inherently complex Chinese herbal medicines for GI cancers. Network pharmacology may effectively improve the development of current omics-based studies by directing treatment protocols based on the underlying biology of tumors. By combining omics assays with network pharmacological studies, future research can effectively incorporate the complex mechanisms of Chinese herbal medicine systems to generate biological networks and simplify the associated disease models. For example, through the integrative analysis of network pharmacology and RNA sequencing, Bushen-Jianpi-Jiedu Decoction combined with oxaliplatin can prolong postoperative CRC patient survival and improve their well-being; this is likely to involve the regulation of multiple signaling pathways (Yang et al., 2021). In addition, numerous public databases provide an unprecedented opportunity to dissect Chinese herbal medicines via bioinformatics analysis. The Traditional Chinese Medicine Information Database (TCMID) and Herb Ingredients’ Targets (HIT) database provide information about Chinese herbs, including formulas, herbal ingredients, therapeutic effects, and clinical indications [Ji et al., 2016 (PMID: 16376038); Yan et al., 2022 (PMID: 34986599)]. The Traditional Chinese Medicine Integrated database (TCMID) displays a network for integrative relationships between herbs and diseases, as well as active ingredients and targets [Huang et al., 2018 (PMID: 29106634)]. TCMID facilitates the study of combination therapy and understanding of the underlying mechanisms for Chinese herbal medicines at the molecular level. Combined with a bioinformatics analysis of public databases and omics, the biological functions and molecular mechanisms of Chinese herbal medicines in the treatment of GI cancers can be identified more accurately. Based on data from omics and public databases, Wu et al. screened out key botanical drugs and targets of Chinese herbal medicines for ulcerative colitis treatment by network pharmacology and enrichment analysis [Wu et al., 2020 (PMID: 32627503)]. By omics and bioinformatics analysis, systematic evaluation of Chinese herbal medicine for the treatment of GI cancers provides a basis for clinical prevention and treatment. Biological data generated by omics assays can be combined with systems biology to reveal the pharmacological substances, action targets, and mechanisms of Chinese herbal medicines (Cai et al., 2018). Thus, the combination of omics approaches and systems biology may contribute to the exploration of Chinese herbal medicines for treating GI cancers.

Author contributions

MH and YF conceived and supervised the project and designed the review. S-YL, W-JW, Q-YL, QY, X-LL, and YY performed the literature review and wrote the manuscript. MH and YF are the corresponding authors. All authors provided their final approval for the publication of this manuscript.

Funding

This work was supported by the National Natural Science Foundation of China (82174319), National Natural Science Foundation of China (82004161), Special Project for Research and Development in Key areas of Guangdong Province (2020B1111100011), Natural Science Foundation of Guangdong Province (2021A1515011320), Major scientific research project of Zhongshan City (2021B3001), Natural Science Foundation of Guangdong province (2021A1515110932), and Guangdong Provincial Key Laboratory of TCM Pathgenesis and Prescriptions of Heart and Spleen Diseases (2022B1212010012).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors, and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

References

Abdelfatah, E., Kerner, Z., Nanda, N., and Ahuja, N. (2016). Epigenetic therapy in gastrointestinal cancer: The right combination. Ther. Adv. Gastroenterol. 9 (4), 560–579. doi:10.1177/1756283X16644247

PubMed Abstract | CrossRef Full Text | Google Scholar

Abdulla, M. A., Fard, A. A., Sabaratnam, V., Wong, K. H., Kuppusamy, U. R., Abdullah, N., et al. (2011). Potential activity of aqueous extract of culinary-medicinal lion's mane mushroom, Hericium erinaceus (bull.: Fr.) pers. (Aphyllophoromycetideae) in accelerating wound healing in rats. Int. J. Med. Mushrooms 13 (1), 33–39. doi:10.1615/intjmedmushr.v13.i1.50

PubMed Abstract | CrossRef Full Text | Google Scholar

Bao, J., Ding, R. B., Jia, X., Liang, Y., Liu, F., Wang, K., et al. (2018). Fast identification of anticancer constituents in Forsythiae Fructus based on metabolomics approaches. J. Pharm. Biomed. Anal. 154, 312–320. doi:10.1016/j.jpba.2018.03.020

PubMed Abstract | CrossRef Full Text | Google Scholar

Cai, F. F., Zhou, W. J., Wu, R., and Su, S. B. (2018). Systems biology approaches in the study of Chinese herbal formulae. Chin. Med. 13, 65. doi:10.1186/s13020-018-0221-x

PubMed Abstract | CrossRef Full Text | Google Scholar

Chai, Y., and Zhao, M. (2017). iTRAQ-based quantitative proteomic analysis of the inhibitory effects of polysaccharides from Viscum coloratum (kom.) nakai on HepG2 cells. Sci. Rep. 7 (1), 4596. doi:10.1038/s41598-017-04417-x

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, P., Li, X., Zhang, R., Liu, S., Xiang, Y., Zhang, M., et al. (2020a). Combinative treatment of beta-elemene and cetuximab is sensitive to KRAS mutant colorectal cancer cells by inducing ferroptosis and inhibiting epithelial-mesenchymal transformation. Theranostics 10 (11), 5107–5119. doi:10.7150/thno.44705

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, Y., Gao, Y., Yi, X., Zhang, J., Chen, Z., and Wu, Y. (2020c). Integration of transcriptomics and metabolomics reveals the antitumor mechanism underlying shikonin in colon cancer. Front. Pharmacol. 11, 544647. doi:10.3389/fphar.2020.544647

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, Y., Zhang, F., Du, Z., Xie, J., Xia, L., Hou, X., et al. (2021). Proteome analysis of Camellia nitidissima Chi revealed its role in colon cancer through the apoptosis and ferroptosis pathway. Front. Oncol. 11, 727130. doi:10.3389/fonc.2021.727130

PubMed Abstract | CrossRef Full Text | Google Scholar

Chen, Y., Wu, X., Liu, C., and Zhou, Y. (2020b). Betulinic acid triggers apoptosis and inhibits migration and invasion of gastric cancer cells by impairing EMT progress. Cell biochem. Funct. 38 (6), 702–709. doi:10.1002/cbf.3537

PubMed Abstract | CrossRef Full Text | Google Scholar

Cheng, Y., Han, X., Mo, F., Zeng, H., Zhao, Y., Wang, H., et al. (2021). Apigenin inhibits the growth of colorectal cancer through down-regulation of E2F1/3 by miRNA-215-5p. Phytomedicine. 89, 153603. doi:10.1016/j.phymed.2021.153603

PubMed Abstract | CrossRef Full Text | Google Scholar

Daddiouaissa, D., Amid, A., Abdullah Sani, M. S., and Elnour, A. A. M. (2021). Evaluation of metabolomics behavior of human colon cancer HT29 cell lines treated with ionic liquid graviola fruit pulp extract. J. Ethnopharmacol. 270, 113813. doi:10.1016/j.jep.2021.113813

PubMed Abstract | CrossRef Full Text | Google Scholar

Dai, R., Liu, M., Xiang, X., Yang, L., Zhichao, X., and Hongxi, X. (2022). OMICS applications for medicinal plants in gastrointestinal cancers: Current advancements and future perspectives. Front. Pharmacol. 13, 842203. doi:10.3389/fphar.2022.842203

PubMed Abstract | CrossRef Full Text | Google Scholar

Dong, X. Z., Song, Y., Lu, Y. P., Hu, Y., Liu, P., and Zhang, L. (2019). Sanguinarine inhibits the proliferation of BGC-823 gastric cancer cells via regulating miR-96-5p/miR-29c-3p and the MAPK/JNK signaling pathway. J. Nat. Med. 73 (4), 777–788. doi:10.1007/s11418-019-01330-7

PubMed Abstract | CrossRef Full Text | Google Scholar

Geisen, U., Zenthoefer, M., Peipp, M., Kerber, J., Plenge, J., Manago, A., et al. (2015). Molecular mechanisms by which a Fucus vesiculosus extract mediates cell cycle inhibition and cell death in pancreatic cancer cells. Mar. Drugs 13 (7), 4470–4491. doi:10.3390/md13074470

PubMed Abstract | CrossRef Full Text | Google Scholar

Geng, Q. S., Liu, R. J., Shen, Z. B., Wei, Q., Zheng, Y. Y., Jia, L. Q., et al. (2021). Transcriptome sequencing and metabolome analysis reveal the mechanism of Shuanghua Baihe Tablet in the treatment of oral mucositis. Chin. J. Nat. Med. 19 (12), 930–943. doi:10.1016/S1875-5364(22)60150-X

PubMed Abstract | CrossRef Full Text | Google Scholar

Gnocchi, D., Del Coco, L., Girelli, C. R., Castellaneta, F., Cesari, G., Sabba, C., et al. (2021). (1 H-NMR metabolomics reveals a multitarget action of Crithmum maritimum ethyl acetate extract in inhibiting hepatocellular carcinoma cell growth. Sci. Rep. 11 (1), 1259. doi:10.1038/s41598-020-78867-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Guo, L., Mei, N., Liao, W., Chan, P. C., and Fu, P. P. (2010). Ginkgo biloba extract induces gene expression changes in xenobiotics metabolism and the Myc-centered network. OMICS 14 (1), 75–90. doi:10.1089/omi.2009.0115

PubMed Abstract | CrossRef Full Text | Google Scholar

Han, S., Wang, Y., Ma, J., Wang, Z., Wang, H. M. D., and Yuan, Q. (2020). Sulforaphene inhibits esophageal cancer progression via suppressing SCD and CDH3 expression, and activating the GADD45B-MAP2K3-p38-p53 feedback loop. Cell Death Dis. 11 (8), 713. doi:10.1038/s41419-020-02859-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Hernandez-Balmaseda, I., Guerra, I. R., Declerck, K., Herrera Isidron, J. A., Perez-Novo, C., Van Camp, G., et al. (2021). Marine seagrass extract of Thalassia testudinum suppresses colorectal tumor growth, motility and angiogenesis by autophagic stress and immunogenic cell death pathways. Mar. Drugs 19 (2), 52. doi:10.3390/md19020052

PubMed Abstract | CrossRef Full Text | Google Scholar

Hou, C., Guo, D., Yu, X., Wang, S., and Liu, T. (2020). TMT-based proteomics analysis of the anti-hepatocellular carcinoma effect of combined dihydroartemisinin and sorafenib. Biomed. Pharmacother. 126, 109862. doi:10.1016/j.biopha.2020.109862

PubMed Abstract | CrossRef Full Text | Google Scholar

Hu, X. Q., Sun, Y., Lau, E., Zhao, M., and Su, S. B. (2016). Advances in synergistic combinations of Chinese herbal medicine for the treatment of cancer. Curr. Cancer Drug Targets 16 (4), 346–356. doi:10.2174/1568009616666151207105851

PubMed Abstract | CrossRef Full Text | Google Scholar

Hua-cheng, L., Jian-gang, W., et al. (2015). The proteomics research of human hepatic carcinoma cell line SMMC-7721 on the effects of Gexia Zhuyu decoction pharmacological serum. Chin. J. Integr. Traditional West. Med. Liver Dis. 25 (03), 156–158+172.

Google Scholar

Jang, M. G., Ko, H. C., and Kim, S. J. (2020). Effects of p-coumaric acid on microRNA expression profiles in SNU-16 human gastric cancer cells. Genes Genomics 42 (7), 817–825. doi:10.1007/s13258-020-00944-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Ji, Q., Luo, Y. Q., Wang, W. h., Liu, X., Li, Q., and Su, S. b. (2016). Research advances in traditional Chinese medicine syndromes in cancer patients. J. Integr. Med. 14 (1), 12–21. doi:10.1016/S2095-4964(16)60237-6

PubMed Abstract | CrossRef Full Text | Google Scholar

Jia, Z., Xie, Y., Wu, H., Wang, Z., Li, A., Li, Z., et al. (2021). Phlorizin from sweet tea inhibits the progress of esophageal cancer by antagonizing the JAK2/STAT3 signaling pathway. Oncol. Rep. 46 (1), 137. doi:10.3892/or.2021.8088

CrossRef Full Text | Google Scholar

Jiang, F., Liu, M., Wang, H., Shi, G., Chen, B., Chen, T., et al. (2020). Wu Mei Wan attenuates CAC by regulating gut microbiota and the NF-kB/IL6-STAT3 signaling pathway. Biomed. Pharmacother. 125, 109982. doi:10.1016/j.biopha.2020.109982

PubMed Abstract | CrossRef Full Text | Google Scholar

Kao, H. H., Wu, C. J., Won, S. J., Shin, J. W., Liu, H. S., and Su, C. L. (2011). Kinase gene expression and subcellular protein expression pattern of protein kinase C isoforms in curcumin-treated human hepatocellular carcinoma Hep 3B cells. Plant Foods Hum. Nutr. 66 (2), 136–142. doi:10.1007/s11130-011-0228-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Karimi, K. Z., Saberi, S., Tsai, K. W., and Mohammadi, M. (2015). MicroRNA-21: Mechanisms of oncogenesis and its application in diagnosis and prognosis of gastric cancer. Arch. Iran. Med. 18 (8), 524–536.

PubMed Abstract | Google Scholar

Khatkov, I. E., Kagramanova, A. V., Zakharzhevskaya, N. B., Babikova, E. A., Generozov, E. V., Shcherbakov, P. L., et al. (2016). Current principles in the screening, diagnosis, and therapy of colorectal cancer. Ter. Arkh. 88 (2), 90–96. doi:10.17116/terarkh201688290-96

PubMed Abstract | CrossRef Full Text | Google Scholar

Kim, H. Y., Lee, S. G., Oh, T. J., Lim, S. R., Kim, S. H., Lee, H. J., et al. (2015). Antiproliferative and apoptotic activity of chamaecyparis obtusa leaf extract against the HCT116 human colorectal cancer cell line and investigation of the bioactive compound by gas chromatography-mass spectrometry-based metabolomics. Molecules 20 (10), 18066–18082. doi:10.3390/molecules201018066

PubMed Abstract | CrossRef Full Text | Google Scholar

Kim, S. H., Jin, H., Meng, R. Y., Kim, D. Y., Liu, Y. C., Chai, O. H., et al. (2019). Activating Hippo pathway via Rassf1 by ursolic acid suppresses the tumorigenesis of gastric cancer. Int. J. Mol. Sci. 20 (19), E4709. doi:10.3390/ijms20194709

PubMed Abstract | CrossRef Full Text | Google Scholar

Lan, H., Wang, H., Gao, M., Luo, G., Zhang, J., Yi, E., et al. (2021). Analysis and construction of a competitive endogenous RNA regulatory network of baicalin-induced apoptosis in human osteosarcoma cells. Biomed. Res. Int. 2021, 9984112. doi:10.1155/2021/9984112

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, F., Yang, X. W., Krausz, K. W., Nichols, R. G., Xu, W., Patterson, A. D., et al. (2015a). Modulation of colon cancer by nutmeg. J. Proteome Res. 14 (4), 1937–1946. doi:10.1021/pr5013152

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, L., Xu, X., Wu, L., Zhu, H., He, Z., Zhang, B., et al. (2019). Scutellaria barbata polysaccharides inhibit tumor growth and affect the serum proteomic profiling of hepatoma H22bearing mice. Mol. Med. Rep. 19 (3), 2254–2262. doi:10.3892/mmr.2019.9862

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, L., Zhao, L. M., Dai, S. L., Cui, W. X., Lv, H. L., Chen, L., et al. (2016). Periplocin extracted from cortex periplocae induced apoptosis of gastric cancer cells via the ERK1/2-EGR1 pathway. Cell. Physiol. biochem. 38 (5), 1939–1951. doi:10.1159/000445555

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, L. G., Yue, G., Lee, J. K. M., Wong, E. C. W., Fung, K. P., Yu, J., et al. (2018). Gene expression profiling reveals the plausible mechanisms underlying the antitumor and antimetastasis effects of Andrographis paniculata in esophageal cancer. Phytother. Res. 32 (7), 1388–1396. doi:10.1002/ptr.6074

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, W. B., Li, Y., Yu, C., and He, Y. M. (2015b). Reversal of multidrug resistance by the Chinese medicine yiqi Jianpi Huaji decoction and the mechanism of action in human gastric cancer SGC7901/VCR cells. Evid. Based. Complement. Altern. Med. 2015, 390812. doi:10.1155/2015/390812

PubMed Abstract | CrossRef Full Text | Google Scholar

Li, Y., Gong, L., Qi, R., Sun, Q., Xia, X., He, H., et al. (2017). Paeoniflorin suppresses pancreatic cancer cell growth by upregulating HTRA3 expression. Drug Des. devel. Ther. 11, 2481–2491. doi:10.2147/DDDT.S134518

PubMed Abstract | CrossRef Full Text | Google Scholar

Liang, M. K., Liang, X. Q., Zhong, J., Wei, Y. T., Lian, Z. P., Huang, Z. K., et al. (2021). Integrative analysis of epigenomics, transcriptomics, and proteomics to identify key targets and pathways of Weining granule for gastric cancer. J. Ethnopharmacol. 270, 113787. doi:10.1016/j.jep.2021.113787

PubMed Abstract | CrossRef Full Text | Google Scholar

Lin, C. S., Kuo, C. L., Wang, J. P., Cheng, J. S., Huang, Z. W., and Chen, C. F. (2007). Growth inhibitory and apoptosis inducing effect of Perilla frutescens extract on human hepatoma HepG2 cells. J. Ethnopharmacol. 112 (3), 557–567. doi:10.1016/j.jep.2007.05.008

PubMed Abstract | CrossRef Full Text | Google Scholar

Lin, L. L., Hsia, C. R., Hsu, C. L., Huang, H. C., and Juan, H. F. (2015). Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells. BMC Genomics 16, 41. doi:10.1186/s12864-015-1230-0

PubMed Abstract | CrossRef Full Text | Google Scholar

Lu, J. J., Lu, D. Z., Chen, Y. F., Dong, Y. T., Zhang, J. R., Li, T., et al. (2015). Proteomic analysis of hepatocellular carcinoma HepG2 cells treated with platycodin D. Chin. J. Nat. Med. 13 (9), 673–679. doi:10.1016/S1875-5364(15)30065-0

PubMed Abstract | CrossRef Full Text | Google Scholar

McFadden, R., Larmonier, M. C. B., Shehab, K. W., Midura-Kiela, M., Ramalingam, R., Harrison, C. A., et al. (2015). The role of curcumin in modulating colonic microbiota during colitis and colon cancer prevention. Inflamm. Bowel Dis. 21 (11), 2483–2494. doi:10.1097/MIB.0000000000000522

PubMed Abstract | CrossRef Full Text | Google Scholar

Menendez, J. A., Papadimitropoulou, A., Vellon, L., and Lupu, R. (2006). A genomic explanation connecting "mediterranean diet", olive oil and cancer: Oleic acid, the main monounsaturated fatty acid of olive oil, induces formation of inhibitory "PEA3 transcription factor-PEA3 DNA binding site" complexes at the her-2/neu (erbB-2) oncogene promoter in breast, ovarian and stomach cancer cells. Eur. J. Cancer 42 (15), 2425–2432. doi:10.1016/j.ejca.2005.10.016

PubMed Abstract | CrossRef Full Text | Google Scholar

Pirih, N., and Kunej, T. (2017). Toward a taxonomy for multi-omics science? Terminology development for whole genome study approaches by omics technology and hierarchy. OMICS 21 (1), 1–16. doi:10.1089/omi.2016.0144

CrossRef Full Text | Google Scholar

Rahbari, M., Rahbari, N., Reissfelder, C., Weitz, J., and Kahlert, C. (2016). Exosomes: Novel implications in diagnosis and treatment of gastrointestinal cancer. Langenbecks Arch. Surg. 401 (8), 1097–1110. doi:10.1007/s00423-016-1468-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Ren, Z., Qin, T., Liu, X., Luo, Y., Qiu, F., Long, Y., et al. (2018). Optimization of Hericium erinaceus polysaccharide-loaded Poly (lactic-co-glycolicacid) nanoparticles by RSM and its absorption in Caco-2 cell monolayers. Int. J. Biol. Macromol. 118, 932–937. doi:10.1016/j.ijbiomac.2018.06.169

PubMed Abstract | CrossRef Full Text | Google Scholar

Shang, H. S., Lu, H. F., Lee, C. H., Chiang, H. S., Chu, Y. L., Chen, A., et al. (2018). Quercetin induced cell apoptosis and altered gene expression in AGS human gastric cancer cells. Environ. Toxicol. 33 (11), 1168–1181. doi:10.1002/tox.22623

PubMed Abstract | CrossRef Full Text | Google Scholar

Shen, K., Xi, Z., Xie, J., Wang, H., Xie, C., Lee, C. S., et al. (2016). Guttiferone K suppresses cell motility and metastasis of hepatocellular carcinoma by restoring aberrantly reduced profilin 1. Oncotarget 7 (35), 56650–56663. doi:10.18632/oncotarget.10992

PubMed Abstract | CrossRef Full Text | Google Scholar

Siyo, V., Schafer, G., Hunter, R., Grafov, A., Grafova, I., Nieger, M., et al. (2017). The cytotoxicity of the ajoene analogue BisPMB in WHCO1 oesophageal cancer cells is mediated by CHOP/GADD153. Molecules 22 (6), E892. doi:10.3390/molecules22060892

PubMed Abstract | CrossRef Full Text | Google Scholar

Sui, H., Zhang, L., Gu, K., Chai, N., Ji, Q., Zhou, L., et al. (2020). YYFZBJS ameliorates colorectal cancer progression in Apc(Min/+) mice by remodeling gut microbiota and inhibiting regulatory T-cell generation. Cell Commun. Signal. 18 (1), 113. doi:10.1186/s12964-020-00596-9

PubMed Abstract | CrossRef Full Text | Google Scholar

Sun, C., Zhang, S., Liu, C., and Liu, X. (2019). Curcumin promoted miR-34a expression and suppressed proliferation of gastric cancer cells. Cancer biother. Radiopharm. 34 (10), 634–641. doi:10.1089/cbr.2019.2874

PubMed Abstract | CrossRef Full Text | Google Scholar

Tan, J., Tian, Y., Cai, R., Yi, T., Jin, D., and Guo, J. (2019). Antiproliferative and proapoptotic effects of a protein component purified from Aspongopus chinensis dallas on cancer cells in vitro and in vivo. Evid. Based. Complement. Altern. Med. 2019, 8934794. doi:10.1155/2019/8934794

PubMed Abstract | CrossRef Full Text | Google Scholar

Tao, Y., Zhan, S., Wang, Y., Zhou, G., Liang, H., Chen, X., et al. (2018). Baicalin, the major component of traditional Chinese medicine Scutellaria baicalensis induces colon cancer cell apoptosis through inhibition of oncomiRNAs. Sci. Rep. 8 (1), 14477. doi:10.1038/s41598-018-32734-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Torre, L. A., Bray, F., Siegel, R. L., Ferlay, J., Lortet-Tieulent, J., and Jemal, A. (2015). Global cancer statistics, 2012 Ca. Cancer J. Clin. 65 (2), 87–108. doi:10.3322/caac.21262

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, C., Zhou, X., Wang, Y., Wei, D., Deng, C., Xu, X., et al. (2017). The antitumor constituents from hedyotis diffusa willd. Molecules 22 (12), E2101. doi:10.3390/molecules22122101

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, C. Z., Huang, W. H., Zhang, C. F., Wan, J. Y., Wang, Y., Yu, C., et al. (2018a). Role of intestinal microbiome in American ginseng-mediated colon cancer prevention in high fat diet-fed AOM/DSS mice [corrected]. Clin. Transl. Oncol. 20 (3), 302–312. doi:10.1007/s12094-017-1717-z

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, L., Ke, J., Wang, C., Li, Y., Wu, G., Ding, Q., et al. (2020). Efficacy and safety of Banxia XieXin decoction, a blended traditional Chinese medicine, as monotherapy for patients with advanced hepatocellular carcinoma. Integr. Cancer Ther. 19, 1534735420942587. doi:10.1177/1534735420942587

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, X. Y., Yin, J. Y., Zhao, M. M., Liu, S. Y., Nie, S. P., and Xie, M. Y. (2018b). Gastroprotective activity of polysaccharide from Hericium erinaceus against ethanol-induced gastric mucosal lesion and pylorus ligation-induced gastric ulcer, and its antioxidant activities. Carbohydr. Polym. 186, 100–109. doi:10.1016/j.carbpol.2018.01.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, X. Y., Zhang, D. D., Yin, J. Y., Nie, S. P., and Xie, M. Y. (2019). Recent developments in Hericium erinaceus polysaccharides: Extraction, purification, structural characteristics and biological activities. Crit. Rev. Food Sci. Nutr. 59, S96–S115. doi:10.1080/10408398.2018.1521370

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, Z., Xu, J., Wang, Y., Xiang, L., and He, X. (2021). Total saponins from Tupistra chinensis baker inhibits growth of human gastric cancer cells in vitro and in vivo. J. Ethnopharmacol. 278, 114323. doi:10.1016/j.jep.2021.114323

PubMed Abstract | CrossRef Full Text | Google Scholar

Wanga, X. Z., Gu, J. L., Gao, M., Bian, Y., Liang, J. Y., Wen, H. M., et al. (2018). Peperomin E induces promoter hypomethylation of metastatic-suppressor genes and attenuates metastasis in poorly differentiated gastric cancer. Cell. Physiol. biochem. 50 (6), 2341–2364. doi:10.1159/000495096

PubMed Abstract | CrossRef Full Text | Google Scholar

Wu, J., Wang, J., Su, Q., Ding, W., Li, T., Yu, J., et al. (2018). Traditional Chinese medicine Astragalus polysaccharide enhanced antitumor effects of the angiogenesis inhibitor apatinib in pancreatic cancer cells on proliferation, invasiveness, and apoptosis. Onco. Targets. Ther. 11, 2685–2698. doi:10.2147/OTT.S157129

PubMed Abstract | CrossRef Full Text | Google Scholar

Wu, J. X., Zhang, X., Zou, X., Wang, M., Wang, H. X., Wang, Y. H., et al. (2019). The effect of Jianpi Yangzheng Xiaozheng Decoction and its components on gastric cancer. J. Ethnopharmacol. 235, 56–64. doi:10.1016/j.jep.2019.02.003

PubMed Abstract | CrossRef Full Text | Google Scholar

Wu, X., Li, X., Wang, W., Shan, Y., Wang, C., Zhu, M., et al. (2020). Integrated metabolomics and transcriptomics study of traditional herb Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao reveals global metabolic profile and novel phytochemical ingredients. BMC Genomics 21 (10), 697. doi:10.1186/s12864-020-07005-y

PubMed Abstract | CrossRef Full Text | Google Scholar

Xiong, L. L., Du, R. L., Chen, J. J., Jiang, Y., Xue, L. L., Niu, R. Z., et al. (2020). Anticolorectal cancer effects of AUCAN: Effects to suppress proliferation, metastasis, and invasion of tumor cells. Biomed. Res. Int. 2020, 9786428. doi:10.1155/2020/9786428

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, M. D., Zhou, W. J., Chen, X. L., Chen, J., Ji, Q., Li, Q., et al. (2021). Therapeutic effect and mechanism of bushen-jianpi-jiedu decoction combined with chemotherapeutic drugs on postoperative colorectal cancer. Front. Pharmacol. 12, 524663. doi:10.3389/fphar.2021.524663

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, P., Jiang, Y., Pan, Y., Ding, X., Rhea, P., Ding, J., et al. (2019). Mistletoe extract Fraxini inhibits the proliferation of liver cancer by down-regulating c-Myc expression. Sci. Rep. 9 (1), 6428. doi:10.1038/s41598-019-41444-2

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, R., Shan, S., Zhang, C., Shi, J., Li, H., and Li, Z. (2020). Inhibitory effects of bound polyphenol from foxtail millet bran on colitis-associated carcinogenesis by the restoration of gut microbiota in a mice model. J. Agric. Food Chem. 68 (11), 3506–3517. doi:10.1021/acs.jafc.0c00370

PubMed Abstract | CrossRef Full Text | Google Scholar

Yang, Y., Zhang, N., Li, K., Chen, J., Qiu, L., and Zhang, J. (2018). Integration of microRNA-mRNA profiles and pathway analysis of plant isoquinoline alkaloid berberine in SGC-7901 gastric cancers cells. Drug Des. devel. Ther. 12, 393–408. doi:10.2147/DDDT.S155993

PubMed Abstract | CrossRef Full Text | Google Scholar

Ying, J., Zhang, M., Qiu, X., and Lu, Y. (2018). The potential of herb medicines in the treatment of esophageal cancer. Biomed. Pharmacother. 103, 381–390. doi:10.1016/j.biopha.2018.04.088

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, Q., Wang, X., Cao, S., Sun, Y., He, X., Jiang, B., et al. (2020). Berberine represses human gastric cancer cell growth in vitro and in vivo by inducing cytostatic autophagy via inhibition of MAPK/mTOR/p70S6K and Akt signaling pathways. Biomed. Pharmacother. 128, 110245. doi:10.1016/j.biopha.2020.110245

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, S., Tang, D., Zang, W., Yin, G., Dai, J., Sun, Y. U., et al. (2017). Synergistic inhibitory effect of traditional Chinese medicine astragaloside IV and curcumin on tumor growth and angiogenesis in an orthotopic nude-mouse model of human hepatocellular carcinoma. Anticancer Res. 37 (2), 465–473. doi:10.21873/anticanres.11338

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, S. H., and Shang, C. (2011). Correlation between traditional Chinese medicine syndromes and omics: A literature review. Zhong Xi Yi Jie He Xue Bao 9 (12), 1286–1291. doi:10.3736/jcim20111202

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhang, W., Liu, Q., Luo, L., Song, J., Han, K., Liu, R., et al. (2021). Use Chou's 5-steps rule to study how Baicalin suppresses the malignant phenotypes and induces the apoptosis of colorectal cancer cells. Arch. Biochem. Biophys. 705, 108919. doi:10.1016/j.abb.2021.108919

PubMed Abstract | CrossRef Full Text | Google Scholar

Zheng, K., Zhao, Q., Chen, Q., Xiao, W., Jiang, Y., and Jiang, Y. (2019). The synergic inhibitory effects of dark tea (Camellia sinensis) extract and p38 inhibition on the growth of pancreatic cancer cells. J. Cancer 10 (26), 6557–6569. doi:10.7150/jca.34637

PubMed Abstract | CrossRef Full Text | Google Scholar

Zhu, H. C., Jia, X. K., Fan, Y., Xu, S. H., Li, X. Y., Huang, M. Q., et al. (2021). Alisol B 23-acetate ameliorates azoxymethane/dextran sodium sulfate-induced male murine colitis-associated colorectal cancer via modulating the composition of gut microbiota and improving intestinal barrier. Front. Cell. Infect. Microbiol. 11, 640225. doi:10.3389/fcimb.2021.640225

PubMed Abstract | CrossRef Full Text | Google Scholar