AUTHOR=Rao Kehan , Qin Shumin , Yang Yuanming , Zhan Kai , Wu Haomeng , Zheng Huan , Huang Shaogang 

TITLE=Shenling Baizhu Powder Alleviates TNBS-Induced Colitis in Rats by Improving Intestinal Epithelial Permeability and Inhibiting Inflammation Through the TLR5/MyD88/NF-κB Pathway

JOURNAL=Frontiers in Pharmacology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.883918

DOI=10.3389/fphar.2022.883918

ISSN=1663-9812

ABSTRACT=<p><bold>Aim of the study:</bold> To evaluate the protective effect and mechanism of shenling baizhu powder (SBP) on TNBS-induced colitis.</p><p><bold>Methods:</bold> Rats were given TNBS to establish the model of colitis and subsequently treated with different doses of SBP or mesalamine (MES). In addition, the expression of the TLR5/MyD88/NF-κB signaling pathway and critical targets of the intestinal mucosal barrier was detected by immunochemical analysis techniques.</p><p><bold>Results:</bold> SBP significantly ameliorated the symptoms of TNBS-induced colitis in rats and reduced the secretion of pro-inflammatory cytokines. SBP could effectively strengthen epithelial barrier integrity in TNBS-induced colitis by increasing the secretion of mucin and tight junction and inhibiting apoptosis. Furthermore, we identified the crucial role of the TLR5/MyD88/NF-κB signaling pathway in exerting the therapeutic effect of SBP.</p><p><bold>Conclusion:</bold> The results of our study suggest that SBP has therapeutic effects on TNBS-induced colitis and potential value in treating and maintaining remission of colitis.</p>