AUTHOR=Xu Tingting , Zheng Jingjing , Jin WeiXing , Li Lu , Lin Luxi , Shaukat Aftab , Zhang Chaodong , Cao Qinqin , Ashraf Muhammad , Huang Shucheng 

TITLE=Total Flavonoids of Rhizoma Drynariae Ameliorate Bone Growth in Experimentally Induced Tibial Dyschondroplasia in Chickens via Regulation of OPG/RANKL Axis

JOURNAL=Frontiers in Pharmacology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.881057

DOI=10.3389/fphar.2022.881057

ISSN=1663-9812

ABSTRACT=<p><bold>Background:</bold><italic>Rhizoma Drynariae</italic>, traditional Chinese herb, is widely used to treat and prevent bone disorders. However, experimental evidence on the use of <italic>Rhizoma Drynariae</italic> extract, total flavonoids of <italic>Rhizoma Drynariae</italic> (TFRD) to treat tibial dyschondroplasia (TD) in chickens and its underlying mechanisms have not been investigated.</p><p><bold>Purpose:</bold> To evaluate the therapeutic effect of TFRD on leg disease caused by TD and elucidate its mechanisms in modulating the bone status.</p><p><bold>Methods:</bold> Thiram-induced chicken TD model has been established. The tibia status was evaluated by analyzing tibia-related parameters including tibial weight, tibial length and its growth plate width and by performing histopathological examination. The expression of tibial bone development-related genes and proteins was confirmed by western blotting and qRT-PCR.</p><p><bold>Results:</bold> The results showed that administration of TFRD mitigated lameness, increased body weight, recuperated growth plate width in broilers affected by TD and the increase of tibia weight and tibia length is significantly positively correlated with body weight. Compared with the TD group broilers, 500 mg/kg TFRD evidently reduced the damage width of the growth plate and improved its blood vessel distribution by elevating the gene expression levels of BMP-2 and Runx2 and OPG/RANKL ratio. Furthermore, correlation analysis found that the damage width of the growth plate was negatively correlated with the expression levels of BMP-2 and OPG.</p><p><bold>Conclusion:</bold> The present study revealed that TFRD could promote the bone growth <italic>via</italic> upregulating OPG/RANKL ratio, suggesting that TFRD might be a potential novel drug in the treatment of TD in chickens.</p>