AUTHOR=Kasarello Kaja , Köhling Iwona , Kosowska Anna , Pucia Katarzyna , Lukasik Anna , Cudnoch-Jedrzejewska Agnieszka , Paczek Leszek , Zielenkiewicz Urszula , Zielenkiewicz Piotr 

TITLE=The Anti-Inflammatory Effect of Cabbage Leaves Explained by the Influence of bol-miRNA172a on FAN Expression

JOURNAL=Frontiers in Pharmacology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.846830

DOI=10.3389/fphar.2022.846830

ISSN=1663-9812

ABSTRACT=<p>Recently, the possibility of cross-kingdom gene expression regulation by miRNAs from other species (“xenomiRs”), specifically from plants, has acquired scientific meaning. Based on the one of oldest methods for dealing with inflammation <italic>via</italic> the use of cabbage leaf compresses, we investigated the effects of <italic>Brassica oleracea</italic> derived miR172a on the potential human target gene encoding FAN (Factor Associated with Neutral Sphingomyelinase Activation) protein. <italic>In vitro</italic> experiments showed a decrease in FAN protein levels in both human and mouse cells transfected with bol-miRNA172a. As the FAN protein mediates inflammatory responses, the potential of miR172a to mitigate the inflammatory process was tested in a mouse model of rheumatoid arthritis. Animal studies showed the decreased oedema of inflamed paws in mouse with rheumatoid arthritis model induced after treatment with miR172a.</p>