AUTHOR=Zhou Yumei , Zhao Haihong , Wang Tieshan , Zhao Xiaoshan , Wang Ji , Wang Qi 

TITLE=Anti-Inflammatory and Anti-asthmatic Effects of TMDCT Decoction in Eosinophilic Asthma Through Treg/Th17 Balance

JOURNAL=Frontiers in Pharmacology

VOLUME=13

YEAR=2022

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.819728

DOI=10.3389/fphar.2022.819728

ISSN=1663-9812

ABSTRACT=<p>Tuo-Min-Ding-Chuan decoction (TMDCT) is a Traditional Chinese Medicine (TCM) formula consisting of twelve herbs that can relieve the symptoms and treat allergic asthma. Yet, the underlying mechanism of action is still unclear. In this study, we investigated the effect of TMDCT in regulating Treg/Th17 cells immune balance and explored potential metabolic and gut biomarkers associated with Treg and Th17 cells in eosinophilic asthma mice treated by TMDCT. We found that TMDCT increases Treg cells percentage and decreases Th17 cells percentage in the ovalbumin (OVA) -induced eosinophilic asthma mice model. Furthermore, Imidazoleacetic acid, <sc>dL</sc>-glutamine, <sc>L</sc>-pyroglutamic acid, 2-deoxy-<sc>d</sc>-glucose were preliminary identified as biomarkers in plasma metabolites treated by TMDCT, meanwhile genus <italic>Desulfovibrio</italic>, genus <italic>Butyricimonas</italic> and genus <italic>Prevotella 9</italic> were preliminary identified as gut microbiota biomarkers after TMDCT treatment. These results provide an experimental foundation for the treatment of allergic asthma with Chinese herbal compounds.</p>