AUTHOR=Nicolas Mael , Lasalo Malia , Chow Sharron , Antheaume Cyril , Huet Karl , Hnawia Edouard , Guillemin Gilles J. , Nour Mohammed , Matsui Mariko 

TITLE=Anti-inflammatory activities of Coleus forsteri (formerly Plectranthus forsteri) extracts on human macrophages and chemical characterization

JOURNAL=Frontiers in Pharmacology

VOLUME=13

YEAR=2023

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2022.1081310

DOI=10.3389/fphar.2022.1081310

ISSN=1663-9812

ABSTRACT=<p><bold>Introduction:</bold> Formerly named <italic>Plectranthus</italic><italic>forsteri</italic>, <italic>Coleus forsteri</italic> (Benth.) A.J.Paton, 2019 is a Lamiaceae traditionally used to treat flu-like symptoms and shock-related ecchymosis, especially in the Pacific region. Few studies investigated chemical composition and anti-inflammatory potential of this plant.</p><p><bold>Method:</bold> Herein, we investigated anti-inflammatory potential of <italic>C. forsteri</italic> ethanolic (ePE) and cyclohexane (cPE) plant extract on LPS-induced human macrophages models and quantified cytokines and quinolinic acid (QUIN) as inflammatory markers.</p><p><bold>Results:</bold> Our results show that extract of ePE and cPE significantly inhibit inflammatory cytokine IL-6 and TNF-α induced by LPS on PMA-derived THP-1 macrophages. QUIN production is also diminished under ePE and cPE treatment in activated human monocyte-derived macrophages (MDMs). Seven abietane diterpenes were characterized from <italic>C. forsteri</italic> cPE including coleon U (<bold>1</bold>), coleon U-quinone (<bold>2</bold>), 8α,9α-epoxycoleon U-quinone (<bold>3</bold>), horminone or 7α-hydroxyroyleanone (<bold>4</bold>), 6β,7α-dihydroxyroyleanone (<bold>5</bold>), 7α-acetoxy-6β-hydroxyroyleanone (<bold>6</bold>) and 7α-formyloxy-6β-hydroxyroyleanone (<bold>7</bold>).</p><p><bold>Discussion:</bold> We discussed potential contributions of these molecules from <italic>C. forsteri</italic> extracts for their anti-inflammatory activities.</p>