Ahmad Reza Aminian1
Mohammad Hossein Boskabady- 1Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
- 2Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Ocimum basilicum L. (O. basilicum) and its constituents show anti-inflammatory, immunomodulatory, and antioxidant effects. The plant has been mainly utilized in traditional medicine for the treatment of respiratory disorders. In the present article, effects of O. basilicum and its main constituents on respiratory disorders, assessed by experimental and clinical studies, were reviewed. Relevant studies were searched in PubMed, Science Direct, Medline, and Embase databases using relevant keywords including “Ocimum basilicum,” “basilicums,” “linalool,” “respiratory disease,” “asthma,” “obstructive pulmonary disease,” “bronchodilatory,” “bronchitis,” “lung cancer,” and “pulmonary fibrosis,” and other related keywords.The reviewed articles showed both relieving and preventing effects of the plant and its ingredients on obstructive pulmonary diseases such as chronic obstructive pulmonary disease (COPD), asthma, and other respiratory disorders such as bronchitis, aspergillosis tuberculosis, and lung cancer. The results of the reviewed articles suggest the therapeutic potential of O. basilicum and its constituent, linalool, on respiratory disorders.
Introduction
Respiratory diseases mainly include chronic obstructive pulmonary disease (COPD), asthma, pneumonia, pulmonary fibrosis, and lung cancer (Ferkol and Schraufnagel, 2014). These diseases affect a large number of people every year and reduce a person’s level of performance in daily activities (Zar and Ferkol, 2014), and they are the most common cause of referral to general practitioners worldwide (Chou et al., 2012). The rate of respiratory dysfunction due to pulmonary diseases depends on the disease type and severity (Bellamy et al., 2006; Pahal and Sharma, 2019). In the last decades, environmental agents such as pollutants and diet as well as individual factors such as genetics and epigenetics resulted in a rise in the prevalence of inflammatory, allergic, malignancy, and immunodeficiency disorders (Gea et al., 2015). These factors may result in a cascade of destructive and inflammatory mechanisms creating the pathological symptom of allergic diseases including asthma (Balkissoon et al., 2011). Early diagnosis, treatment, prevention, and monitoring of the respiratory disorders as well as the development of new therapeutics to alleviate such pathological mechanisms and restore the balance of the immune system are required for curing respiratory diseases (Gholamnezhad et al., 2015). Inflammatory obstructive respiratory diseases are treated mainly by two types of drugs, including relieving drugs that reduce airway obstruction and preventive drugs that reduce lung inflammation (Rabe and Schmidt, 2001).
Currently, two types of drugs are used to relieve symptoms and prevent the progression of the disease severity in various respiratory disorders such as asthma and COPDs. The relieving drugs used for the treatment of asthma are beta-2 agonists, anticholinergics, methylxanthines, corticosteroids (mainly inhaled forms), leukotriene modifiers, mast cell stabilizers, and immunomodulators (Mullane, 2011). Various immunomodulators are used for the treatment of respiratory diseases, including omalizumab, an anti-IgE antibody; benralizumab, mepolizumab, and reslizumab, IL-5 antibodies; and dupilumab, an IL-4 and IL-13 inhibitor monoclonal antibody which is used for the treatment of allergic asthma; some other antibodies are used for the treatment of other respiratory disorders such as lung infection and lung cancer (Desoubeaux et al., 2016).
However, drugs that are currently used for the treatment of respiratory diseases lack full therapeutic efficacy and show major side effects. Therefore, the development of new drugs with higher efficacy and fewer side effects for the treatment of respiratory disorders is needed (Mullane, 2011). Herbal medicines are safe agents that are used as alternative and complementary therapeutics. Ocimum basilicum L. (O. basilicum) or basil is an important essential oil crop, medicinal plant, and culinary herb that belongs to the Lamiaceae family (Figure 1); it is an herbal plant wildly cultivated in regions of Central and Southeast Asia such as Iran and Pakistan (Sajjadi, 2006; Gholamnezhad et al., 2019). O. basilicum is a well-known herb traditionally used in different cultures across the globe for the management of lung diseases and other organ disorders (Boskabady et al., 2006).
FIGURE 1. Natural view of Ocimum basilicum.
O. basilicum is used to manage lung disorders and dyspnea in Iran (Noumi, 2009); asthma in Cameroon (Nath et al., 2008); bronchitis, cough, and asthma in India (Lemos et al., 2016); acute lung diseases, including bronchitis, cough, and sore throat, in Brazil (Janbaz et al., 2014); and tuberculosis and acute lung diseases in Ethiopia (Fujita et al., 1999). The bioactive ingredients of the plant depend on the growth regions (Driesen et al., 2021; Khater et al., 2021). The effects of O. basilicum and its derivatives on dermal pathology and wound healing including acne, eczema, boils, psoriasis, and rashes were reported (Fritea et al., 2021).
O. basilicum is usually used for the treatment of some disorders related to the respiratory tract including asthma, bronchitis, cough, gastrointestinal disorders (Othman et al., 2021; Touiss et al., 2021), cardiovascular diseases (Janbaz et al., 2014; Abidoye et al., 2022), neurocognitive disorders (Heshami et al., 2021), and metabolic disorders (Kiyose et al., 2021).
Several pharmacological impacts have been shown for O. basilicum, including anti-inflammatory (Neamati et al., 2016; Złotek et al., 2016; Saeidi et al., 2018), antioxidant (Eftekhar et al., 2018a; Teofilović et al., 2021), and bronchodilatory properties (Janbaz et al., 2014; Figure 2).
FIGURE 2. Effects of Ocimum basilicum and linalool on respiratory disease.
O. basilicum flowers and leaves are consumed in the form of infusion, syrup, decoction, as a sudorific, stimulant, carminative, diuretic, and febrifuge agent, and are frequently suggested for bronchitis and coughs (Vieira and Simon, 2000).
O. basilicum leaves have a strong antioxidant effect due to the existence of some constituents such as eugenol and vicenin (Ziaei et al., 2014). In addition, this plant showed anti-inflammatory properties due to the presence of citronellol, limonene, and eugenol in its leaf (Ziaei et al., 2014).
Phytochemical analysis and pharmacological studies showed that linalool is the main constituent of O. basilicum concerning both the amount and pharmacological activities of the plant (Rezzoug et al., 2019). Linalool metabolites are excreted in urine as free forms or conjugates. Products of linalool reduction (dihydro- and tetrahydrolinalool) were identified in rodent urine. Some of the bronchodilatory properties of this plant are mainly related to linalool metabolites such as alpha-terpineol, nerol, and geraniol. A significant proportion of orally administered linalool follows intermediary metabolic pathways as shown in Figure 3 (Elsharif et al., 2015).
FIGURE 3. Linalool molecular structure and metabolites.
The aim of the current article was to review experimental and clinical effects of O. basilicum and its main constituent, linalool, on respiratory disorders.
Materials and Methods
Appropriate experimental and clinical studies were searched in PubMed, Science Direct, Medline, and Embase databases using relevant keywords including “Ocimum,” “Ocimum basilicum,” “basilicums,” “linalool,”, “respiratory disease,” “asthma,” “obstructive pulmonary disease,” “bronchodilatory,” “bronchitis,” “lung cancer,” and “pulmonary fibrosis,” and other related keywords published from 1989 until September 2020. The searched keywords were selected based on the MeSH alone and combined. The reviewed articles were available in full-text and written in English in well-recognized journals.
Results
The Main Ingredients of O. basilicum
O. basilicum has more than 100 bioactive ingredients including various vitamins, electrolytes, minerals, and phytonutrients (Marwat et al., 2011; Da Costa et al., 2015). The chemical compositions of essential oils (Özcan and Chalchat, 2002) of O. basilicum include methyl chavicol (estragole), cineole, eugenol, methyl eugenol, elemicin, myristicin, rosmarinic acid, linalool, apigenin, ursolic acid, and methyl cinnamate (Mullane, 2011; Amer et al., 2021; Senthoorraja et al., 2021).
Also, monoterpenes (ocimene, geraniol, and camphor), sesquiterpenes (bisabolene and caryophyllene), and phenylpropanoids (methyl cinnamate and methyl eugenol) are present in varying amounts and strongly influence the flavor of the plant. Linalool is a monoterpenoid which is octa-1, 6-diene substituted by methyl groups at positions 3 and 7, and a hydroxyl group at position 3 (Letizia et al., 2003). The presence of anthocyanins, anthraquinones, tannins, reducing sugars, glycosides, proteins, amino acids, flavonoids, and volatile oils, and the non-appearance of terpinoids and alkaloids were demonstrated as constituents of O. basilicum as well as the presence of steroids in an aqueous extract of the plant. Also, it was observed that the antioxidants and anticancer compounds are higher in the aqueous extract of the plant (Alkhateeb et al., 2021). Various chemical and physical conditions could influence the constituents of the plant. The promotion of the stress condition of metals, aluminum, lead, and cadmium in O. basilicum resulted in the reduction of the dry matter mass and increased the synthesis of phenolic compounds (Do Prado et al., 2022). The rosmarinic acid (RA) content of O. basilicum also increased due to the application of chitosan lactate (Hawrylak-Nowak et al., 2021). In addition, applications of aromatic amino acid composition reduced the expression of the TAT gene in the leaves, and the contents of chicoric acid, methyl chavicol, caffeic acid, and vanillic acid were increased (Kisa et al., 2021). The hairy root production increased in O. basilicum (green basil “Cinnamon”) when it was cultured under the light but it increased in purple basil (Purpurascens) when it was cultured under dark conditions. However, the content of RA in the cultured hairy roots of green basil was higher than that of purple basil under both light and dark conditions (Kwon et al., 2021). It was shown that excess zinc decreased chlorophyll content, total phenol and total flavonoid contents, as well as TBARS levels, which indicate an oxidative burst in O. basilicum (Mahmoudi et al., 2021). A study also indicated that O. basilicum grown on media supplemented with copper oxide nanoparticles (CuO-NPs) increased phenolic contents, flavonoid contents, and antioxidant activities compared to manganese oxide nanoparticles (MnO-NPs) and control. Superoxide dismutase (SOD) and peroxidase (POD) activities were also higher in CuO-NP cultures than in MnO-NPs and control. A negative impact of contaminated soil with cadmium (Cd) and lead (Pb) on morphological trait development of basil was shown. Cultivated plants with Cd- and Pb-contaminated soils showed a major influence on the EOs’ yield, composition, and phytoremediation of the soil (Youssef, 2021). In addition, RA, chicoric acid, and eugenol were higher when cultured in CuO-NP media (Nazir et al., 2021). Increase salicylic acid and its β-glucoside cause to accumulation of jasmonic and abscisic acids in O. basilicum due to foliar-spraying treatment with U. intestinalis extract and the essential oil (EO) chemotype changed from methyl eugenol/eugenol to epi-α-cadinol and increased sesquiterpenes (Paulert et al., 2021). Table 1 summarizes different constituents of O. basilicum.
TABLE 1. Chemical composition of O. basilicum.
The Effects of O. basilicum and Its Main Constituent on Respiratory Disorders: Experimental Evidence
Relaxant Effect of O. basilicum and Its Main Constituent on Tracheal Smooth Muscle
O. basilicum has been revealed to possess bronchodilatory and relaxant effects. Macerated hydroethanolic extract of O. basilicum leaf (0.75, 1.50, and 3.00 mg/ml) meaningfully decreased the tracheal smooth muscle (TSM) contractile tone induced by methacholine, KCl, and airways inflammation in a dose-dependent manner (Pérez-Guerrero et al., 1997; Eftekhar et al., 2019a).
It seems that if O. basilicum extracts affected potassium channels, they should not have any effect on the TSM contracted by KCl, whereas the extract should affect TSM when contracted by methacholine. While KCl acts on the calcium channels (Pérez-Guerrero et al., 1997) and calcium channel blockers showed a bronchodilatory effect (Mccaig and De Jonckheere, 1993; Miyahara et al., 1993; Janbaz et al., 2014), no calcium channel blocking effects for this extract were shown (S. S. Athari et al., 2016).
The concentration-dependent relaxant effects of four cumulative concentrations of Soxhlet and macerated extracts of O. basilicum (0.25, 0.5, 0.75, and 1.0 W/V) on the contractile tone of tracheal smooth muscle, induced by methacholine and KCl in guinea pigs, were found to be comparable to those of theophylline (Boskabady et al., 2005).
Linalool and eugenol, the constituents of O. basilicum, were also shown to have a relaxing effect on TSM or a bronchodilator effect similar to the plant itself (Shabir et al., 2014; Silveira et al., 2017). The relaxation effects of eugenol and linalool (100 mM) on TSM in the absence and presence of three inhibitors indicated that the main mechanism responsible for the relaxant effect of linalool is its inhibitory effect on cyclooxygenase signaling cascades, and that for eugenol is its ROS inhibitory effect.
It was shown that linalool fully recovered the electromechanical-induced contraction in TSM with intact and denuded epithelium, but the effect was lower in TSM with intact epithelium. Linalool inhibits the calcium and barium influx curves. This study showed that the relaxant effect of linalool was mediated by the blocking effect on calcium influx through voltage-dependent channels. These results also indicate that linalool and eugenol are responsible for the relaxant effect of O. basilicum on TSM.
Other likely mechanisms for bronchodilatory effects of O. basilicum are stimulating β-adrenergic but blocking histamine H1 receptors, anticholinergic function, inducing inhibitory nerves other than cholinergic system (NANC), or prevention of stimulatory NANC, methylxanthine similar activity, as well as phosphodiesterase inhibitory effect (S. S. Athari et al., 2016).
These findings showed the relaxant effect of essential oils of O. basilicum on TSM which was suggested to be due to the presence of linalool and eugenol. The possible mechanisms of the relaxant effect of O. basilicum and its constituents are stimulating β-adrenergic, inhibiting histamine H1 and muscarinic receptors, blocking calcium channels, methylxanthine similar activity, and phosphodiesterase inhibitory effect. Therefore, the plant and its constituents could be used as bronchodilatory agents in obstructive pulmonary diseases. A summary on the relaxant effects of O. basilicum and its ingredients is presented in Table 2.
TABLE 2. Summarized experimental effects of O. basilicum on the respiratory system.
Preventive Effect of O. basilicum and Its Main Constituent on Respiratory Disorders
Asthma and Allergy
Allergic asthma is a complex illness of the lung characterized by wheezing, coughing, eosinophilia, dyspnea, and lung inflammation (Ying et al., 1999). The bronchial constriction, mucus hyper-secretion, and enhanced airway responsiveness are important properties in asthma pathophysiology (S. S. Athari et al., 2017). Interleukin (IL)-4, IL-13, IL-5, and Th2 cytokines have a significant role in asthma development (Mould et al., 2000).
Th1/Th2 imbalance toward increasing Th2 activity appears in asthma pathogenesis. Cytokines released from Th2 cells like IL-5 and IL-4 stimulate IgE secretion from the B cells that play an important role in allergic asthma. Secretion of phospholipase A2 establishes lung inflammation in asthma by arachidonic acid releasing, cytokine diffusion, lysophospholipid production, and the effect of inflammatory and immunologic cells. Medicines currently used for asthma decrease lung inflammation and bronchospasm; however, following termination of treatment, symptoms recur. For a long time, asthma has been treated using herbal medicines in the Middle East, China, and Europe.
Treatment of BALB/c mice with asthma induced by the ovalbumin (OVA, 20 μg, i.p) with O. basilicum (orally, on days 25, 27, and 29) suppressed Th2 cytokine gene expression (IL-4, 5, and 13) (S. M. Athari et al., 2018). Treatment with O. basilicum decreased goblet cell hyperplasia and mucus hyper-secretion in the asthmatic lung segments (S. M. Athari et al., 2018). In this study, Athari et al. described that O. basilicum might increase Th1 cytokine gene expression and Th1/Th2 cytokine balance. This balance is essential for the management and treatment of allergic asthma. Thus, immunomodulatory and anti-inflammatory effects of O. basilicum in allergic asthma were shown, and it was suggested that this plant could be considered for the treatment of asthma (S. M. Athari et al., 2018). In another study, it has been reported that oral administration of O. basilicum seed extract (on days 25, 27, and 29 from 30 days of sensitization period) inhibits the expression of Th2 upstream cytokines such as IL-8, IL-9, IL-11, IL-25, IL-33, and eotaxin and hyperplasia in goblet cell in an animal model of OVA-induced allergic asthma. These findings also revealed that therapeutic effects of the plant in asthma were induced by the inhibition of the Th2 expression and goblet cell hyperplasia (Nasaba et al., 2020).
In a rat model of asthma induced by OVA, O. basilicum hydroethanolic extract (0.75, 1.50, and 3.00 mg/ml) in drinking water, for 21 days during the sensitization period, reduced IL-4, PLA2, IgE, and total protein (TP) levels but enhanced the IFN-γ/IL-4 ratio in a dose-dependent manner. Lung pathological changes in asthmatic animals were also improved by the plant extract. The results of this study suggest the therapeutic potential of O. basilicum in asthma by improving lung pathological, inflammatory, and immunological changes in sensitized rats (Eftekhar et al., 2019b). In rats sensitized to OVA, treatment with the extract of O. basilicum (0.75, 1.5, and 3.0 mg/ml), rosmarinic acid (0.125, 0.25, and 0.5 mg/ml) administered in the animals’ drinking water, and dexamethasone (1.25 μg/ml) decreased total and differential white blood cells (WBC) and improved serum levels of antioxidant and oxidant markers; these effects were comparable with the effect of dexamethasone. In another study with a similar methodology, tracheal responsiveness to OVA and methacholine; total WBC count; percentages of monocytes, eosinophils, and neutrophils; and levels of oxidant markers in the BALF were markedly reduced, but antioxidant markers were enhanced due to treatment with the extract of O. basilicum; these effects were similar to the effect of dexamethasone in sensitized rats. Treatment of asthmatic rats induced by OVA with rosmarinic acid (0.125, 0.25, and 0.5 mg/ml) also reduced the tracheal responsiveness to OVA and methacholine; total and differential WBC count, and levels of oxidant markers in the BALF increased, but levels of antioxidant markers decreased, which were similar to dexamethasone effects. In asthmatic rats, rosmarinic acid (0.125, 0.250, and 0.500 mg/ml) decreased the levels of IL-4, PLA2, IgE, and total protein, and increased the IFN-/IL-4 ratio in the BALF. Lung pathological changes induced by OVA were improved by rosmarinic acid treatment (Shakeri et al., 2019). The findings of these works suggest a therapeutic effect for the extract of O. basilicum and its constituent, rosmarinic acid, on asthma (Eftekhar et al., 2018a; Eftekhar et al., 2018b; Eftekhar et al., 2019a).
O. basilicum and its constituents, including linalool, eugenol, and more than 5 oxygenated monoterpene derivatives, have an anti-inflammatory effect (Okoye et al., 2014). O. basilicum terpenoids have been reported to suppress nuclear factor kappa B (NF-κβ) signaling and nitric oxide (NO) production, thus producing anti-inflammatory effects. NO is an inflammatory molecule that plays a role in immunoregulation. NO inhibitors are of therapeutic importance in preventing pathological conditions caused by inflammation. On the other hand, the inhibition of the c-Jun subunit of the activator protein 1 (AP-1) suppresses transcription of inflammatory genes, leading to the inhibition of the inflammatory pathways. Anti-inflammatory effect of the Ocimum genus was shown to be induced through inhibition of pro-inflammatory cytokines (Kapewangolo et al., 2015). Also, the fixed oils of the Ocimum genus may have the potential to inhibit the cyclooxygenase and lipoxygenase pathways. Agents that affect the pathways of the arachidonic acid metabolism would be of great value in inflammatory conditions such as asthma (Singh et al., 1996). Iranian O. basilicum is used for improvement of pharynx congestion, fever, and stomachache.
Treatment with O. basilicum suppressed Th2 cytokines’ (IL-4, 5, IL-8, IL-9, IL-11, 13 IL-25, and IL-33) gene expression; oxidant markers; total and differential WBC both in the blood and the BALF; reduced PLA2, IgE, and TP; alleviated mucus hyper-secretion and lung pathological changes (including goblet cell hyperplasia and airway responsiveness); and enhanced Th1 cytokine gene expression and Th1/Th2 balance but increased antioxidant marker levels in an animal model of asthma. Rosmarinic acid, a constituent of the plant, also showed similar effects as the plant. O. basilicum terpenoids also suppressed NF-κβ signaling and the NO level. Therefore, since O. basilicum and its constituents suppressed lung inflammation as the main characteristic of asthma, they could be considered candidates for the prevention of asthma.
Chronic Obstructive Pulmonary Disease
COPD is a type of obstructive respiratory illness categorized by long-term breathing complications and poor airflow (Macnee, 2005). The main signs of the disease are cough with sputum production and shortness of breath (Agusti et al., 2010) which deteriorates over time (Mannino and Buist, 2007).
There is limited information about the effect of O. basilicum on COPDs. However, it is reported that O. basilicum, O. gratissimum, and O. tenuiflorum are the three herbal plants of the Ocimum genus which inhibit apoptosis caused by H2O2-oxidative stress in respiratory cells, showing potential therapeutic effects against COPDs (Vardhan, 2013).
The average cell viability was meaningfully different between the groups treated with the three plants as follows: O. tenuiflorum (holy basil) (90.25%), O. gratissimum (wild basil) (75.49%), and O. basilicum (sweet basil) (74.28%). Therefore, O. tenuiflorum had superior effects than O. gratissimum and O. basilicum which showed similar potential.
In vitro, linalool was reported to inhibit endotoxin-induced production of TNF-α and IL-1β in RAW264.7 macrophage, and TNF-α, IL-1β, nitric oxide, and prostaglandin E2 in a murine microglial cell line BV2 (Lee et al., 2018). Linalool also attenuated lung inflammation in COPDs, inhibited the infiltration of inflammatory cells, and reduced IL-1β, IL-6, IL-8, TNF-α, and monocyte chemoattractant protein (MCP)-1 production. Also, linalool inhibited lung myeloperoxidase (MPO) activity and pathological changes. Linalool suppressed NF-κB activation in a dose-dependent manner. Linalool also inhibits cigarette smoke–induced lung inflammation by inhibiting NF-κB activation. Besides, eugenol causes the inhibition of pro-inflammatory mediators such as COX-2, NF-κB, IL-6, leukotriene C4, and 5-LOX (De Araújo Lopes et al., 2018).
Therefore, this plant could be of therapeutic value on COPDs due to its antioxidant constituents. The effect of O. basilicum and its constituents on asthma and COPDs shown by experimental studies is presented in Table 2.
Bronchitis
Bronchitis is a lung infection primarily caused by the rhinovirus, influenza virus, coronavirus, adenovirus, and other viruses (Amber et al., 2017) in which the mucous membrane of the bronchial tracts develops inflammation and causes defect in oxygen transfer from the trachea to the lungs (Pierangeli et al., 2007).
The bioactive ingredients of O. basilicum have been described to prevent different viral infections. Three types of secondary metabolites including linalool, apigenin, and ursolic acid have been described against various types of human adenovirus complications in bronchitis. Among these, ursolic acid displayed extreme inhibition (50%) of adenovirus. Triterpenoids such as saponin suppressed the viral activity by inhibiting the DNA and viral protein capsid synthesis (Amber et al., 2017).
Lung Cancer
Lung cancer, or lung carcinoma, is a malignant respiratory tumor categorized by uncontrolled cell growth in lung tissue (Barta et al., 2019). These growths can be extended by the progression of metastasis into close tissue or other parts of the body (Ridge et al., 2013). Two chief types of such cancers are small-cell lung carcinoma and non–small-cell lung carcinoma. The most common signs of lung cancer are cough, shortness of breath, chest pain, and weight loss (Baumann et al., 2009; Makinoshima et al., 2012).
In a study, Arshad Qamar et al. (2010) described the antiproliferative activity of extract of aerial parts of O. basilicum (40 mg/ml) with dimethyl sulfoxide (DMSO) in MCF-7 cells which might partially be due to the impacts of ursolic acid on microtubules and F-actin. Ursolic acid (40, 60, and 100 μM) induced F-actin aggregation after 24 h of incubation. This may be explained by either inhibition of depolymerization of actin filaments or stabilization and promotion of actin polymerization.
The molecular mechanism of ursolic acid–induced apoptosis may possibly involve the release of apoptogenic molecules, in particular both AIF and Endo G, from mitochondria in NCI-H292 human lung cancer cells (Chen et al., 2019).
Also, the aqueous extract of O. gratissimum suppressed the viability of A549 cells, which might be due to the inhibition of antiapoptotic cascades and activation of apoptotic signaling, suggesting that the aqueous O. gratissimum extract might be helpful in lung carcinoma management (Arshad Qamar et al., 2010).
O. basilicum, O. gratissimum, and O. tenuiflorum inhibited apoptosis caused by H2O2-oxidative stress in respiratory cells, indicating their potential as a possible medication for COPD. O. basilicum constituents including linalool, apigenin, and ursolic acid can prevent different viral infections including human adenovirus bronchitis. The antiproliferative effect of O. basilicum in MCF-7 cells and lung carcinoma was shown to be partially due to the impacts of ursolic acid. Therefore, this plant could be of therapeutic value against COPDs, bronchitis, and lung cancer. The effect of O. basilicum and its ingredient on bronchitis and lung cancer reported by experimental studies is presented in Table 2.
The Effects of O. basilicum on Respiratory Disorders: Clinical Evidence
Pulmonary Aspergillosis
Aspergillus spp. frequently causes human bronchopulmonary infections, mostly in immunocompromised patients (Hedayati et al., 2016; Taghizadeh-Armaki et al., 2017a; Pinto et al., 2018; Omran et al., 2019). It causes a broad spectrum of infections including allergic bronchopulmonary aspergillosis, chronic pulmonary aspergillosis, aspergilloma, and invasive aspergillosis, the most serious clinical issue (Nasri et al., 2015; Taghizadeh-Armaki et al., 2017b).
Aspergillosis comprises various diseases produced by the members of the Aspergillus genus such as A. flavus, A. fumigatus, A. niger, and A. terreus that are the common species accountable for infections (Bahkali et al., 2015).
Bahkali et al. (2015) reported that O. basilicum (20 mg/ml for 7 days) generally prevented the hyphae growth and spore germination of A. fumigatus and A. terreus. Also, this extract exhibited a strong antifungal property versus the hyphae growth and spore germination of A. versicolor and A. flavus.
Also, it has been reported that essential oil extracted from O. basilicum leaves (0.4 μl/ml) has strong antifungal activity versus several aflatoxigenic fungi that contaminate foods, including A. flavus, Fusarium oxysporum, Alternaria alternata, A. fumigatus, Curvularia lunata, Penicillium italicum, A. niger, and F. nivale (Singh et al., 2011).
Linalool can interfere in the biosynthesis of the cell wall and/or increase the ionic permeability of the fungal cell membrane. It may be regarded as an agent capable of controlling fungal infections; for instance, antifungal activity of linalool in cases of Candida spp. isolated from individuals with oral candidiasis was shown (Dias et al., 2017).
Lung Tuberculosis
Tuberculosis is caused by mycobacterial strains; for example, Mycobacterium tuberculosis, M. africanum, and M. bovis, identified cooperatively as the M. tuberculosis complex (Haouat et al., 2012).
It has been indicated that 80% methanolic extracts of O. basilicum seed (75 μg/ml solvent) exhibit promising antimycobacterial activity versus M. tuberculosis and M. bovis strains (Gemechu et al., 2013). This might be due to O. basilicum bioactive ingredients such as saponins, tannins, alkaloids, flavonoids, and polyphenols that exist in the extract (Arya, 2011; Radji et al., 2015). Although the antimicrobial potential of O. basilicum had been described on several other human pathogenic bacteria (Nibret and Wink, 2010; Hussien et al., 2011; Sharaf et al., 2021), no study assessed its effect on Mycobacterium tuberculosis.
Another study reported that inhibition of M. tuberculosis by pure O. basilicum aerial parts’ extract (6.25 μg/ml solvent) supports the usage of this herb as a complementary medicine for improvement of the signs of respiratory tuberculosis (Siddiqui et al., 2012).
The essential oil of O. basilicum revealed antifungal and antibacterial properties (Dube et al., 1989; Siddiqui et al., 2007). O. basilicum extracts and vital oils can also be used to manage lung infections. O. basilicum essential oil (16 μg/ml solvent) prevents arachidonic acid metabolism including cyclooxygenase and lipoxygenase pathways. The watery essence of O. basilicum suppressed giant cell generation in Molt-4 cells with and without HIV-1 infection, indicating its inhibitory activity against HIV-1 reverse transcriptase (Yamasaki et al., 1998).
O. basilicum showed a strong antifungal effect including suppression of the hyphae growth and spore germination of A. versicolor, A. flavus, and other fungi. The antimycobacterial effect of O. basilicum on M. tuberculosis and M. bovis strains was also shown. Therefore, O. basilicum and its constituents could be used for the management of lung infection and lung cancer.
Antibacterial effects of linalool have been shown in several works. Linalool destroys the membrane integrity and increases membrane permeability. Also, considering membrane potential, linalool caused cell membrane depolarization and irregular cell metabolism activity. A study regarding antibacterial activity of linalool against Pseudomonas aeruginosa showed respiratory chain dehydrogenase inhibition that finally led to cell death. Table 3 illustrates various clinical effects of O. basilicum and its ingredients in respiratory disorders.
TABLE 3. Summarized clinical effects of O. basilicum on respiratory diseases.
Toxicity and Safety of O. basilicum
Acute toxicity of hydroalcoholic extract of O. basilicum (50, 500, 1,000, and 2000 mg/kg, orally) has been evaluated for 14 days in Wistar rats, and its sub-chronic toxicity was examined by administering 50, 200, and 500 mg/kg of extract for 45 days. Various variables including body weight changes, food and water consumption, clinical signs, hematological and biochemical parameters, as well as mortality, were monitored during the study period. At the end of the study, weight of the liver and left kidney and liver, histological markers were also assessed. The LD50 of O. basilicum in the acute study was higher than 5 mg/kg. In the sub-chronic study, a reduction in the hematocrit, platelets, and RBC was observed, but no adverse effects on serum parameters and in other variables were seen. The results showed no death or any abnormal dose-dependent changes in biochemical and liver histopathological parameters in the sub-chronic oral administration of O. basilicum in Wistar rats, but several hematological changes had occurred. The results indicated the negligible risk of oral O. basilicum consumption (Rasekh et al., 2012).
In another study, acute and subacute toxicity of O. basilicum oils in rats was studied following a 14-day gavage administration in Wistar rats, and the animal’s general behavior and survival were assessed daily, and their stomach and liver histological analyses were done at the end of the study. Doses higher than 1,500 mg/kg of the oils significantly changed the stomach and liver histology of rats, but no adverse effect was observed. Therefore, the results indicated that the oil of O. basilicum can be considered safe for humans at recommended doses (Fandohan et al., 2008).
Acute toxicity of O. gratissimum (Ocimum oil), the other plant of this family, was examined by oral and intraperitoneal administration of its graded doses as a 4% v/v (LD50 and LD100). Sub-chronic toxicity of the oil was evaluated by the administration of three graded sub-lethal doses for 30 days, and organs and blood samples were taken at the end of the study. In the acute toxicity study, a dose-dependent sedative effect of Ocimum oil was seen in both mice and rats, and the sub-chronic test was done only in rats. There were significant weight changes in the testes, hearts, kidneys, intestines, and lungs as well as significant changes in blood biochemical and hematological variables. Therefore, Ocimum oil has toxic potentialities at higher doses and might be better tolerated when administered orally for systemic delivery (Orafidiya et al., 2004).
Ocimum (2000, 4,000, 6,000, and 8,000 mg/kg, orally) was administered to Swiss mice for acute toxicity assessment, and death and body weight changes were monitored for 7 days. However, for subacute toxicity assessment, rats were treated with three doses of 250, 500, and 1,000 mg/kg/day for 42 consecutive days, and biochemical and hematological variables, and organs’ histology were assessed at the end of the study. The extract up to 8,000 mg/kg did not show mortality or significant changes in general behavior. Body and organ weights, feeding habits, or behavior also did not change in the sub-chronic study, and no toxicity was observed in hematological or blood biochemistry variables or organs’ histology. These results showed non-toxic effects of acute and sub-chronic intake of leaf aqueous extract of Ocimum (Tan et al., 2008).
Discussion
Various studies showed the relaxant effects of O. basilicum and its main constituents including linalool and eugenol, and showed the bronchodilatory effects of the plant and its components against obstructive pulmonary diseases with various possible mechanisms.
The preventive effects of O. basilicum and its constituents such as linalool, eugenol, rosmarinic acid, and terpenoids against asthma were also reported, and several possible mechanisms were shown. The extracts of the plant and its constituents decreased mucus hyper-secretion and goblet cell hyperplasia, inhibited the cyclooxygenase and lipoxygenase pathways, as well as suppressed nuclear factor kappa B (NF-κβ) signaling and nitric oxide (NO) generation in a mouse model of asthma induced by OVA. Lung pathological changes and airway responsiveness to OVA and methacholine were also reduced by the plant and its components in a rat model of asthma. O. basilicum and its derivatives also reduced TNF-α, IL-1β, and IL-2, and inducible nitric oxide synthase (iNOS). In addition, the gene expression of Th2 cytokines (IL-4, 5, and 13) was suppressed and Th1/Th2 cytokine balance was increased in asthmatic animals. All these findings strongly suggest the possible therapeutic effect of the plant and its constituents on asthma. O. basilicum and its constituents, and other species from Ocimum genus, showed potent antioxidant and anti-inflammatory effects which could be considered for the treatment of COPDs.
The extract of O. basilicum prevents MCF-7 cell proliferation partially due to the effects of ursolic acid on microtubules and F-actin. The aqueous extract of O. gratissimum also suppressed the viability of A549 cells through apoptotic signaling activation and antiapoptotic cascade inhibition. Therefore, the plant and its compounds could be used as an anticancer therapy in lung cancers.
The preventive effect of O. basilicum on germination of A. fumigatus and A. terreus as well as its antifungal property on the hyphae growth and spore germination of A. versicolor and A. flavus, and the antifungal activity of the plant on several aflatoxigenic fungi and foods contaminated with A. flavus, Fusarium oxysporum, Alternaria alternata, A. fumigatus, Curvularia lunata, Penicillium italicum, A. niger, and F. nivale were shown; these effects suggested the effect of this plant on pulmonary infections and aspergillosis. The extracts of O. basilicum seed showed antimycobacterial activity against M. tuberculosis due to its ingredients including tannins, saponins, alkaloids, flavonoids, and polyphenols. The inhibition of M. tuberculosis by O. basilicum aerial part extract was also shown. These findings suggest the effect of this plant as a complementary medicine for the treatment of respiratory tuberculosis.
The studies of the plant safety also showed a mild toxicity only when very high doses of the plant were tested. Therefore, O. basilicum preparations are safe when usual doses are used.
Conclusion
The current review showed the therapeutic effects of O. basilicum and its ingredients on various respiratory diseases. The findings of the reviewed articles revealed both relieving and prevention of the plant preparations on obstructive pulmonary diseases including COPDs and asthma. The preventive effects of O. basilicum and its main constituents on other respiratory disorders such as bronchitis, aspergillosis, tuberculosis, and lung cancer were also indicated.
There are limited numbers of clinical studies regarding the effects of the plant and its constituents on respiratory disorders. From the results of the reviewed articles, the average dose of the plant required for the treatment of respiratory disorders was estimated to be 10 mg/kg/day. In addition, the results of the reviewed articles indicate that O. basilicum and its constituents can increase the quality of life of patients with respiratory disorders both as a reliever and preventive medication, and could show synergetic effect with the currently used chemical drugs.
However, further experimental works are needed on the effect of the plant and its constituents on some respiratory disorders such as COPDs, bronchitis, and lung cancer. Before the plant and its constituents could be used in clinical practice, further clinical trials are needed to examine these effects. The exact doses of O. basilicum preparation for the treatment of various respiratory diseases should also be determined in further studies. Whether the plant and its derivatives alone could be used to treat respiratory diseases should be clarified in further studies. Figure 4 summarizes the O. basilicum effect on respiratory disorders.
FIGURE 4. Summary of O. basilicum effect on respiratory disorders.
Author Contributions
MB, study conception and design and manuscript preparation; AA, data collection; RM, data collection, interpretation of results, and manuscript preparation.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Publisher’s Note
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors, and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
References
Abidoye, A. O., Ojedokun, F. O., Fasogbon, B. M., and Bamidele, O. P. (2022). Effects of Sweet Basil Leaves (Ocimum Basilicum L) Addition on the Chemical, Antioxidant, and Storage Stability of roselle Calyces (Hibiscus sabdariffa) Drink. Food Chem. 371, 131170. doi:10.1016/j.foodchem.2021.131170
Agusti, A., Calverley, P. M., Celli, B., Coxson, H. O., Edwards, L. D., Lomas, D. A., et al. (2010). Characterisation of COPD Heterogeneity in the ECLIPSE Cohort. Respir. Res. 11, 122. doi:10.1186/1465-9921-11-122
Alkhateeb, M. A., Al-Otaibi, W. R., Algabbani, Q., Alsakran, A. A., Alnafjan, A. A., Alotaibi, A. M., et al. (2021). Low-temperature Extracts of Purple Blossoms of Basil (Ocimum Basilicum L.) Intervened Mitochondrial Translocation Contributes Prompted Apoptosis in Human Breast Cancer Cells. Biol. Res. 54, 2. doi:10.1186/s40659-020-00324-0
Amber, R., Adnan, M., Tariq, A., and Mussarat, S. (2017). A Review on Antiviral Activity of the Himalayan Medicinal Plants Traditionally Used to Treat Bronchitis and Related Symptoms. J. Pharm. Pharmacol. 69, 109–122. doi:10.1111/jphp.12669
Amer, A., Ghoneim, M., Shoala, T., and Mohamed, H. I. (2021). Comparative Studies of Eco-Friendly Compounds like Humic Acid, Salicylic, and Glycyrrhizic Acids and Their Nanocomposites on French Basil (Ocimum Basilicum L. Cv. Grand Verde). Environ. Sci. Pollut. Res. Int. 28, 47196–47212. doi:10.1007/s11356-021-14022-1
Arshad Qamar, K., Dar, A., S. Siddiqui, B., Kabir, N., Aslam, H., Ahmed, S., et al. (2010). Anticancer Activity of Ocimum Basilicum and the Effect of Ursolic Acid on the Cytoskeleton of MCF-7 Human Breast Cancer Cells. Lddd 7, 726–736. doi:10.2174/1570180811007010726
Athari, S. S., Athari, S. M., Beyzay, F., Movassaghi, M., Mortaz, E., and Taghavi, M. (2017). Critical Role of Toll-like Receptors in Pathophysiology of Allergic Asthma. Eur. J. Pharmacol. 808, 21–27. doi:10.1016/j.ejphar.2016.11.047
Athari, S. S., Pourpak, Z., Folkerts, G., Garssen, J., Moin, M., Adcock, I. M., et al. (2016). Conjugated Alpha-Alumina Nanoparticle with Vasoactive Intestinal Peptide as a Nano-Drug in Treatment of Allergic Asthma in Mice. Eur. J. Pharmacol. 791, 811–820. doi:10.1016/j.ejphar.2016.10.014
Bahkali, A. H., Elgorban, A. M., Samawaty, A. E.-R. M. A. E., Almogren, H.-M. A., Metwally, M. A. E., and Al-Harbi, N. S. (2015). In Vitro Susceptibility of Clinical Aspergillus Species to Some Antifungal Agents. Int. J. Pharmacol. 11, 496–501. doi:10.3923/ijp.2015.496.501
Balkissoon, R., Lommatzsch, S., Carolan, B., and Make, B. (2011). Chronic Obstructive Pulmonary Disease: a Concise Review. Med. Clin. North. Am. 95, 1125–1141. doi:10.1016/j.mcna.2011.08.009
Barta, J. A., Powell, C. A., and Wisnivesky, J. P. (2019). Global Epidemiology of Lung Cancer. Ann. Glob. Health 85. doi:10.5334/aogh.2419
Baumann, P., Nyman, J., Hoyer, M., Wennberg, B., Gagliardi, G., Lax, I., et al. (2009). Outcome in a Prospective Phase II Trial of Medically Inoperable Stage I Non-small-cell Lung Cancer Patients Treated with Stereotactic Body Radiotherapy. J. Clin. Oncol. 27, 3290–3296. doi:10.1200/JCO.2008.21.5681
Bellamy, D., Bouchard, J., Henrichsen, S., Johansson, G., Langhammer, A., Reid, J., et al. (2006). International Primary Care Respiratory Group (IPCRG) Guidelines: Management of Chronic Obstructive Pulmonary Disease (COPD). Prim. Care Respir. J. 15, 48–57. doi:10.1016/j.pcrj.2005.11.003
Bobakulov, K., Ozek, G., Ozek, T., Asilbekova, D. T., Abdullaev, N. D., Sagdullaev, S. S., et al. (2020). Essential Oils and Lipids from the Flowers of Two Varieties of Ocimum Basilicum L. Cultivated in Uzbekistan. J. EssEntial oil rEsEarch 32, 323–330. doi:10.1080/10412905.2020.1749946
Boskabady, M. H., Aslani, M. R., and Kiani, S. (2006). Relaxant Effect of Thymus Vulgaris on guinea-pig Tracheal Chains and its Possible Mechanism(s). Phytother Res. 20, 28–33. doi:10.1002/ptr.1796
Boskabady, M. H., Kiani, S., and Haghiri, B. (2005). Relaxant Effects of Ocimum Basilicum on guinea Pig Tracheal Chains and its Possible Mechanism(s). DARU J. Pharm. Sci. 13, 28–33.
Chaaban, S. B., Hamdi, S. H., Mahjoubi, K., and Jemâa, J. M. B. (2019). Composition and Insecticidal Activity of Essential Oil from Ruta Graveolens, Mentha Pulegium and Ocimum Basilicum against Ectomyelois Ceratoniae Zeller and Ephestia Kuehniella Zeller (Lepidoptera: Pyralidae). J. Plant Dis. Prot. 126, 237–246. doi:10.1007/s41348-019-00218-8
Chen, C. J., Shih, Y. L., Yeh, M. Y., Liao, N. C., Chung, H. Y., Liu, K. L., et al. (2019). Ursolic Acid Induces Apoptotic Cell Death through AIF and Endo G Release through a Mitochondria-dependent Pathway in NCI-H292 Human Lung Cancer Cells In Vitro. In Vivo 33, 383–391. doi:10.21873/invivo.11485
Chou, C. H., Hwang, C. L., and Wu, Y. T. (2012). Effect of Exercise on Physical Function, Daily Living Activities, and Quality of Life in the Frail Older Adults: a Meta-Analysis. Arch. Phys. Med. Rehabil. 93, 237–244. doi:10.1016/j.apmr.2011.08.042
Da Costa, A. S., Arrigoni-Blank, M. D. F., Carvalho Filho, J. L. S. D., De Santana, A. D. D., Santos, D. D. A., Alves, P. B., et al. (2015). Chemical Diversity in Basil (Ocimum sp.) Germplasm. Scientific World J. 2015, 352638. doi:10.1155/2015/352638
De Araújo Lopes, A., Da Fonseca, F. N., Rocha, T. M., De Freitas, L. B., Araújo, E. V. O., Wong, D. V. T., et al. (2018). Eugenol as a Promising Molecule for the Treatment of Dermatitis: Antioxidant and Anti-inflammatory Activities and its Nanoformulation. Oxidative Med. Cell. longevity 2018, 8194849. doi:10.1155/2018/8194849
Desoubeaux, G., Reichert, J. M., Sleeman, M., Reckamp, K. L., Ryffel, B., Adamczewski, J. P., et al. (2016). Therapeutic Monoclonal Antibodies for Respiratory Diseases: Current Challenges and Perspectives. MAbs 8 (6), 999–1009. March 31–April 1, 2016, Tours, France. doi:10.1080/19420862.2016.1196521
Dias, I. J., Trajano, E. R. I. S., Castro, R. D., Ferreira, G. L. S., Medeiros, H. C. M., and Gomes, D. Q. C. (2017). Antifungal Activity of Linalool in Cases of Candida Spp. Isolated from Individuals with Oral Candidiasis. Braz. J. Biol. 78, 368–374. doi:10.1590/1519-6984.171054
Do Prado, N. B., De Abreu, C. B., Pinho, C. S., Junior, M. M. N., Silva, M. D., Espino, M., et al. (2022). Application of Multivariate Analysis to Assess Stress by Cd, Pb and Al in Basil (Ocimum Basilicum L.) Using Caffeic Acid, Rosmarinic Acid, Total Phenolics, Total Flavonoids and Total Dry Mass in Response. Food Chem. 367, 130682. doi:10.1016/j.foodchem.2021.130682
Driesen, E., De Proft, M., and Saeys, W. (2021). Soil Moisture Levels Affect the Anatomy and Mechanical Properties of Basil Stems (Ocimum Basilicum L.). Plants (Basel) 10, 1320. doi:10.3390/plants10071320
Dube, S., Upadhyay, P. D., and Tripathi, S. C. (1989). Antifungal, Physicochemical, and Insect-Repelling Activity of the Essential Oil of Ocimum Basilicum. Can. J. Bot. 67, 2085–2087. doi:10.1139/b89-264
Eftekhar, N., Moghimi, A., and Boskabady, M. H. (2018b). Prophylactic Effect of Rosmarinic Acid on Tracheal Responsiveness, white Blood Cell Count and Oxidative Stress Markers in Lung Lavage of Sensitized Rats. Pharmacol. Rep. 70, 119–125. doi:10.1016/j.pharep.2017.08.010
Eftekhar, N., Moghimi, A., and Boskabady, M. H. (2018a). The Effects of Ocimum Basilicum Extract and its Constituent, Rosmarinic Acid on Total and Differential Blood WBC, Serum Levels of NO, MDA, Thiol, SOD, and CAT in Ovalbumin Sensitized Rats. Iran J. Pharm. Res. 17, 1371–1385.
Eftekhar, N., Moghimi, A., Hossein Boskabady, M., Kaveh, M., and Shakeri, F. (2019a). Ocimum Basilicum Affects Tracheal Responsiveness, Lung Inflammatory Cells and Oxidant-Antioxidant Biomarkers in Sensitized Rats. Drug Chem. Toxicol. 42, 286–294. doi:10.1080/01480545.2018.1459672
Eftekhar, N., Moghimi, A., Mohammadian Roshan, N., Saadat, S., and Boskabady, M. H. (2019b). Immunomodulatory and Anti-inflammatory Effects of Hydro-Ethanolic Extract of Ocimum Basilicum Leaves and its Effect on Lung Pathological Changes in an Ovalbumin-Induced Rat Model of Asthma. BMC Complement. Altern. Med. 19, 349. doi:10.1186/s12906-019-2765-4
El Mokhtari, K., El Brouzi, A., M'hammed, E., and Talbi, M. (2019). Extraction and Composition of the Essential Oil of Ocimum Basilicum Collected in Morocco. J. Anal. Sci. Appl. Biotechnol. 1 (2), 2045–2048.
Elsharif, S. A., Banerjee, A., and Buettner, A. (2015). Structure-odor Relationships of Linalool, Linalyl Acetate and Their Corresponding Oxygenated Derivatives. Front. Chem. 3, 57. doi:10.3389/fchem.2015.00057
Elsherbiny, E. A., El Khateeb, A. Y., and Azzaz, N. A. (2016). Chemical Composition and Fungicidal Effects of Ocimum Basilicum Essential Oil on Bipolaris and Cochliobolus Species. J. Agric. Sci. Technology 18, 1143–1152.
Fandohan, P., Gnonlonfin, B., Laleye, A., Gbenou, J. D., Darboux, R., and Moudachirou, M. (2008). Toxicity and Gastric Tolerance of Essential Oils from Cymbopogon Citratus, Ocimum Gratissimum and Ocimum Basilicum in Wistar Rats. Food Chem. Toxicol. 46, 2493–2497. doi:10.1016/j.fct.2008.04.006
Ferkol, T., and Schraufnagel, D. (2014). The Global burden of Respiratory Disease. Ann. Am. Thorac. Soc. 11, 404–406. doi:10.1513/AnnalsATS.201311-405PS
Fritea, L., Ganea, M., Zdrinca, M., Dobjanschi, L., Antonescu, A., Vicas, S. I., et al. (2021). Perspectives on the Combined Effects of Ocimum Basilicum and Trifolium Pratense Extracts in Terms of Phytochemical Profile and Pharmacological Effects. Plants 10, 1390. doi:10.3390/plants10071390
Fujita, J., Ohtsuki, Y., Suemitsu, I., Shigeto, E., Yamadori, I., Obayashi, Y., et al. (1999). Pathological and Radiological Changes in Resected Lung Specimens in Mycobacterium avium Intracellulare Complex Disease. Eur. Respir. J. 13, 535–540. doi:10.1183/09031936.99.13353599
Gea, J., Pascual, S., Casadevall, C., Orozco-Levi, M., and Barreiro, E. (2015). Muscle Dysfunction in Chronic Obstructive Pulmonary Disease: Update on Causes and Biological Findings. J. Thorac. Dis. 7, E418–E438. doi:10.3978/j.issn.2072-1439.2015.08.04
Gemechu, A., Giday, M., Worku, A., and Ameni, G. (2013). In Vitro Anti-mycobacterial Activity of Selected Medicinal Plants against Mycobacterium tuberculosis and Mycobacterium Bovis Strains. BMC Complement. Altern. Med. 13, 291. doi:10.1186/1472-6882-13-291
Gholamnezhad, Z., Shakeri, F., Saadat, S., Ghorani, V., and Boskabady, M. H. (2019). Clinical and Experimental Effects of Nigella Sativa and its Constituents on Respiratory and Allergic Disorders. Avicenna J. Phytomed 9, 195–212.
Gholamnezhad, Z., Keyhanmanesh, R., and Boskabady, M. H. (2015). Anti-inflammatory, Antioxidant, and Immunomodulatory Aspects of Nigella Sativa for its Preventive and Bronchodilatory Effects on Obstructive Respiratory Diseases: A Review of Basic and Clinical Evidence. J. Funct. Foods 17, 910–927. doi:10.1016/j.jff.2015.06.032
Haouat, A. C., Sqalli, H., Haggoud, A., Ennabili, A., Ibnsouda, S., and Iraqui, M. (2012). Extra-and Intracellular Antimycobacterial Activity of Arbutus Unedo L. Afr. J. Microbiol. Res. 6, 1283–1290. doi:10.5897/AJMR11.1540
Hawrylak-Nowak, B., Dresler, S., Rubinowska, K., and Matraszek-Gawron, R. (2021). Eliciting Effect of Foliar Application of Chitosan Lactate on the Phytochemical Properties of Ocimum Basilicum L. And Melissa Officinalis L. Food Chem. 342, 128358. doi:10.1016/j.foodchem.2020.128358
Hedayati, M. T., Mahdavi Omran, S., Soleymani, A., and Taghizadeh Armaki, M. (2016). Aflatoxins in Food Products in Iran: A Review of the Literature. Jundishapur J. Microbiol. 9, e33235. doi:10.5812/jjm.33235
Heshami, N., Mohammadali, S., Komaki, A., Tayebinia, H., Karimi, J., Abbasi Oshaghi, E., et al. (2021). Favorable Effects of Dill Tablets and Ocimum Basilicum L. Extract on Learning, Memory, and Hippocampal Fatty Acid Composition in Hypercholesterolemic Rats. Iran J. Basic Med. Sci. 24, 300–311. doi:10.22038/ijbms.2021.49013.11230
Hussien, J., Teshale, C., and Mohammed, J. (2011). Assessment of the Antimicrobial Effects of Some Ethiopian Aromatic Spice and Herb Hydrosols. Int. J. Pharmacol. 7, 635–640. doi:10.3923/ijp.2011.635.640
Janbaz, K. H., Hamid, I., and Qadir, M. I. (2014). Spasmolytic, Bronchodilator and Vasodilator Activities of Aqueous-Methanolic Extract of Ocimum Basilicum. Int. J. Agric. Biol. 16, 321–327.
Kadan, S., Saad, B., Sasson, Y., and Zaid, H. (2016). In Vitro evaluation of Anti-diabetic Activity and Cytotoxicity of Chemically Analysed Ocimum Basilicum Extracts. Food Chem. 196, 1066–1074. doi:10.1016/j.foodchem.2015.10.044
Kapewangolo, P., Omolo, J. J., Bruwer, R., Fonteh, P., and Meyer, D. (2015). Antioxidant and Anti-inflammatory Activity of Ocimum Labiatum Extract and Isolated Labdane Diterpenoid. J. Inflamm. (Lond) 12, 4. doi:10.1186/s12950-015-0049-4
Kavoosi, G., and Amirghofran, Z. (2017). Chemical Composition, Radical Scavenging and Anti-oxidant Capacity of Ocimum Basilicum Essential Oil. J. EssEntial oil rEsEarch 29, 189–199. doi:10.1080/10412905.2016.1213667
Khater, E.-S., Bahnasawy, A., Abass, W., Morsy, O., El-Ghobashy, H., Shaban, Y., et al. (2021). Production of Basil (Ocimum Basilicum L.) under Different Soilless Cultures. Scientific Rep. 11, 1–14. doi:10.1038/s41598-021-91986-7
Kisa, D., İmamoğlu, R., Genç, N., Şahin, S., Qayyum, M. A., and Elmastaş, M. (2021). The Interactive Effect of Aromatic Amino Acid Composition on the Accumulation of Phenolic Compounds and the Expression of Biosynthesis-Related Genes in Ocimum Basilicum. Physiol. Mol. Biol. Plants 27, 2057–2069. doi:10.1007/s12298-021-01068-1
Kiyose, C., Takeuchi, H., Yabe, Y., Koike, T., Sakiya, K., Nagase, M., et al. (2021). Improvement Effect of Sweet Basil (Ocimum Basilicum L.) Powder Intake on Obese Mice Fed a High-Fat and High-Sucrose Diet. J. Oleo Sci. 70, 1317–1323. doi:10.5650/jos.ess21139
Kumar, A., Shukla, R., Singh, P., Prakash, B., and Dubey, N. K. (2011). Chemical Composition of Ocimum Basilicum L. Essential Oil and its Efficacy as a Preservative against Fungal and Aflatoxin Contamination of Dry Fruits. Int. J. Food Sci. Technol. 46, 1840–1846. doi:10.1111/j.1365-2621.2011.02690.x
Kwon, D. Y., Kim, Y. B., Kim, J. K., and Park, S. U. (2021). Production of Rosmarinic Acid and Correlated Gene Expression in Hairy Root Cultures of green and Purple Basil (Ocimum Basilicum L.). Prep. Biochem. Biotechnol. 51, 35–43. doi:10.1080/10826068.2020.1789990
Lee, S. C., Wang, S. Y., Li, C. C., and Liu, C. T. (2018). Anti-inflammatory Effect of Cinnamaldehyde and Linalool from the Leaf Essential Oil of Cinnamomum Osmophloeum Kanehira in Endotoxin-Induced Mice. J. Food Drug Anal. 26, 211–220. doi:10.1016/j.jfda.2017.03.006
Lemos, I. C. S. s., Delmondes, G. d. A., Santos, A. D. F. d., Santos, E. S., Oliveira, D. R. d., Figueiredo, P. R. L. d., et al. (2016). Ethnobiological Survey of Plants and Animals Used for the Treatment of Acute Respiratory Infections in Children of a Traditional Community in the Municipality of Barbalha, Ceará, Brazil. Ajtcam 13, 166–175. doi:10.21010/ajtcam.v13i4.22
Letizia, C. S., Cocchiara, J., Lalko, J., and Api, A. M. (2003). Fragrance Material Review on Linalool. Food Chem. Toxicol. 41, 943–964. doi:10.1016/s0278-6915(03)00015-2
Macnee, W. (2005). Treatment of Stable COPD: Antioxidants. Eur. Respir. Rev. 14, 12–22. doi:10.1183/09058180.05.00009402
Mahmoudi, H., Salah, I. B., Zaouali, W., Zorrig, W., Smaoui, A., Ali, T., et al. (2021). Impact of Zinc Excess on Germination, Growth Parameters and Oxidative Stress of Sweet Basil (Ocimum Basilicum L.). Bull. Environ. Contam. Toxicol. 106, 899–907. doi:10.1007/s00128-021-03188-6
Makinoshima, H., Ishii, G., Kojima, M., Fujii, S., Higuchi, Y., Kuwata, T., et al. (2012). PTPRZ1 Regulates Calmodulin Phosphorylation and Tumor Progression in Small-Cell Lung Carcinoma. BMC cancer 12, 537. doi:10.1186/1471-2407-12-537
Mannino, D. M., and Buist, A. S. (2007). Global burden of COPD: Risk Factors, Prevalence, and Future Trends. Lancet 370, 765–773. doi:10.1016/S0140-6736(07)61380-4
Marwat, S. K., Khan, M. S., Ghulam, S., Anwar, N., Mustafa, G., and Usman, K. (2011). Phytochemical Constituents and Pharmacological Activities of Sweet Basil-Ocimum Basilicum L.(Lamiaceae). Asian J. Chem. 23, 3773.
Masoume Athari, S., Mehrabi Nasab, E., and Shamsadin Athari, S. (2018). Study Effect of Ocimum Basilicum Seeds on Mucus Production and Cytokine Gene Expression in Allergic Asthma Mice Model. Revue Française d'Allergologie 58, 489–493. doi:10.1016/j.reval.2018.08.003
Mccaig, D., and De Jonckheere, S. (1993). Effects of Two Ca2+ Modulators in normal and Albumin-Sensitized guinea-pig Trachea. Eur. J. Pharmacol. 249, 53–63. doi:10.1016/0014-2999(93)90661-z
Mehdizadeh, T., Hashemzadeh, M., Nazarizadeh, A., Neyriz-Naghadehi, M., Tat, M., Ghalavand, M., et al. (2016). Chemical Composition and Antibacterial Properties of Ocimum Basilicum, Salvia Officinalis and Trachyspermum Ammi Essential Oils Alone and in Combination with Nisin. Res. J. Pharmacognosy 3, 51–58.
Miyahara, Y., Kizawa, Y., Sano, M., and Murakami, H. (1993). Effects of Organic and Inorganic Ca(2+)-Antagonists on Acetylcholine-Induced Contraction in Molluscan (Mytilus edulis) Smooth Muscle. Gen. Pharmacol. 24, 1419–1423. doi:10.1016/0306-3623(93)90429-2
Mota, I., Sánchez-Sánchez, J., Pedro, L. G., and Sousa, M. J. (2020). Composition Variation of the Essential Oil from Ocimum Basilicum L. Cv. Genovese Gigante in Response to Glomus Intraradices and Mild Water Stress at Different Stages of Growth. Biochem. Syst. Ecol. 90, 104021. doi:10.1016/j.bse.2020.104021
Mould, A. W., Ramsay, A. J., Matthaei, K. I., Young, I. G., Rothenberg, M. E., and Foster, P. S. (2000). The Effect of IL-5 and Eotaxin Expression in the Lung on Eosinophil Trafficking and Degranulation and the Induction of Bronchial Hyperreactivity. J. Immunol. 164, 2142–2150. doi:10.4049/jimmunol.164.4.2142
Mullane, K. (2011). The Increasing challenge of Discovering Asthma Drugs. Biochem. Pharmacol. 82, 586–599. doi:10.1016/j.bcp.2011.06.033
Nasaba, E. M., Athari, S. M., Motlagh, B., and Athari, S. S. (2020). Effects of Oral Administration of Ocimum Basilicum on Goblet Cell Hyperplasia and Upstream Cytokine Gene Expression in Allergic Asthma. Revue Française d'Allergologie 60. doi:10.1016/j.reval.2019.02.226
Nasri, T., Hedayati, M. T., Abastabar, M., Pasqualotto, A. C., Armaki, M. T., Hoseinnejad, A., et al. (2015). PCR-RFLP on β-tubulin Gene for Rapid Identification of the Most Clinically Important Species of Aspergillus. J. Microbiol. Methods 117, 144–147. doi:10.1016/j.mimet.2015.08.007
Nath, K., Deka, P., Borkataki, S., and Borthakur, S. (2008). Traditional Remedies of Respiratory Disorders from Assam, India. Pleione 2, 211–216.
Nazir, S., Jan, H., Zaman, G., Khan, T., Ashraf, H., Meer, B., et al. (2021). Copper Oxide (CuO) and Manganese Oxide (MnO) Nanoparticles Induced Biomass Accumulation, Antioxidants Biosynthesis and Abiotic Elicitation of Bioactive Compounds in Callus Cultures of Ocimum Basilicum (Thai Basil). Artif. Cell Nanomedicine, Biotechnol. 49, 626–634. doi:10.1080/21691401.2021.1984935
Neamati, A., Talebi, S., Hosseini, M., Hossein Boskabady, M., and Beheshti, F. (2016). Administration of Ethanolic Extract of Ocimum Basilicum Leaves Attenuates Depression like Behavior in the Rats Sensitized by Ovalbumin. Cnf 12, 72–78. doi:10.2174/1573401311666151030213446
Nibret, E., and Wink, M. (2010). Volatile Components of Four Ethiopian Artemisia Species Extracts and Their In Vitro Antitrypanosomal and Cytotoxic Activities. Phytomedicine 17, 369–374. doi:10.1016/j.phymed.2009.07.016
Noumi, E. (2009). Treating Asthma with Medicinal Plants. An Ethnomedicinal Case Study from Baré-Bakem, Nkongsamba Region, Cameroon. Syllabus Rev. 1, 10–15.
Okoye, F. B. C., Obonga, W. O., Onyegbule, F. A., Ndu, O. O., and Ihekwereme, C. P. (2014). Chemical Composition and Anti-inflammatory Activity of Essential Oils from the Leaves of Ocimum Basilicum L. And Ocimum Gratissimum L.(Lamiaceae). Int. J. Pharm. Sci. Res. 5, 2174. doi:10.13040/IJPSR.0975
Omran, S. M., Taghizadeh-Armaki, M., Zarrinfar, H., Hedayati, M. T., Abastabar, M., Moqarabzadeh, V., et al. (2019). In-vitro Antifungal Susceptibility Testing of Lanoconazole and Luliconazole against Aspergillus flavus as an Important Agent of Invasive Aspergillosis. J. Infect. Chemother. 25, 157–160. doi:10.1016/j.jiac.2018.07.018
Orafidiya, L. O., Agbani, E. O., Iwalewa, E. O., Adelusola, K. A., and Oyedapo, O. O. (2004). Studies on the Acute and Sub-chronic Toxicity of the Essential Oil of Ocimum Gratissimum L. Leaf. Phytomedicine 11, 71–76. doi:10.1078/0944-7113-00317
Othman, M. S., Khaled, A. M., Al-Bagawi, A. H., Fareid, M. A., Ghany, R. A., Habotta, O. A., et al. (2021). Hepatorenal Protective Efficacy of Flavonoids from Ocimum Basilicum Extract in Diabetic Albino Rats: A Focus on Hypoglycemic, Antioxidant, Anti-inflammatory and Anti-apoptotic Activities. Biomed. Pharmacother. 144, 112287. doi:10.1016/j.biopha.2021.112287
Özcan, M., and Chalchat, J.-C. (2002). Essential Oil Composition of Ocimum Basilicum L. Czech J. Food Sci. 20, 223–228.
Pahal, P., and Sharma, S. (2019). “Chronic Obstructive Pulmonary Disease (COPD) Compensatory Measure,” in StatPearls [Internet] (Treasure Island (FL):: StatPearls Publishing).
Paulert, R., Ascrizzi, R., Malatesta, S., Berni, P., Noseda, M. D., Mazetto De Carvalho, M., et al. (2021). Ulva Intestinalis Extract Acts as Biostimulant and Modulates Metabolites and Hormone Balance in Basil (Ocimum Basilicum L.) and Parsley (Petroselinum crispum L.). Plants 10, 1391. doi:10.3390/plants10071391
Pérez-Guerrero, C., Suárez, J., Herrera, M. D., and Marhuenda, E. (1997). Spasmolytic Effects of Tetrazepam on Rat Duodenum and guinea-pig Ileum. Pharmacol. Res. 35, 493–497. doi:10.1006/phrs.1997.0173
Pierangeli, A., Gentile, M., Di Marco, P., Pagnotti, P., Scagnolari, C., Trombetti, S., et al. (2007). Detection and Typing by Molecular Techniques of Respiratory Viruses in Children Hospitalized for Acute Respiratory Infection in Rome, Italy. J. Med. Virol. 79, 463–468. doi:10.1002/jmv.20832
Pinto, E., Monteiro, C., Maia, M., Faria, M. A., Lopes, V., Lameiras, C., et al. (2018). Aspergillus Species and Antifungals Susceptibility in Clinical Setting in the North of Portugal: Cryptic Species and Emerging Azoles Resistance in A. fumigatus. Front. Microbiol. 9, 1656. doi:10.3389/fmicb.2018.01656
Rabe, K. F., and Schmidt, D. T. (2001). Pharmacological Treatment of Asthma Today. Eur. Respir. J. Suppl. 34, 34s–40s. doi:10.1183/09031936.01.00252501
Radji, M., Kurniati, M., and Kiranasari, A. (2015). Comparative Antimycobacterial Activity of Some Indonesian Medicinal Plants against Multi-Drug Resistant Mycobacterium tuberculosis. J. Appl. Pharm. Sci. 5, 019–022. doi:10.7324/JAPS.2015.50104
Rasekh, H. R., Hosseinzadeh, L., Mehri, S., Kamli-Nejad, M., Aslani, M., and Tanbakoosazan, F. (2012). Safety Assessment of Ocimum Basilicum Hydroalcoholic Extract in Wistar Rats: Acute and Subchronic Toxicity Studies. Iran J. Basic Med. Sci. 15, 645–653.
Rezzoug, M., Bakchiche, B., Gherib, A., Roberta, A., FlaminiGuido, Ö., Kilinçarslan, Ö., et al. (2019). Chemical Composition and Bioactivity of Essential Oils and Ethanolic Extracts of Ocimum Basilicum L. And Thymus Algeriensis Boiss. & Reut. from the Algerian Saharan Atlas. BMC Complement. Altern. Med. 19, 146. doi:10.1186/s12906-019-2556-y
Ridge, C. A., Mcerlean, A. M., and Ginsberg, M. S. (2013). Epidemiology of Lung Cancer. Semin. Intervent Radiol. 30 (2), 93–98. doi:10.1055/s-0033-1342949
Saeidi, F., Sajjadi, S. E., and Minaiyan, M. (2018). Anti-Inflammatory Effect of Ocimum Basilicum Linn. Seeds Hydroalcoholic Extract and Mucilage on Acetic Acid-Induced Colitis in Rats. J. Rep. Pharm. Sci. 7, 295–305.
Sajjadi, S. E. (2006). Analysis of the Essential Oils of Two Cultivated Basil (Ocimum Basilicum L.) from Iran. DARU J. Pharm. Sci. 14, 128–130.
Senthoorraja, R., Subaharan, K., Manjunath, S., Pragadheesh, V. S., Bakthavatsalam, N., Mohan, M. G., et al. (2021). Electrophysiological, Behavioural and Biochemical Effect of Ocimum Basilicum Oil and its Constituents Methyl Chavicol and Linalool on Musca domestica L. Environ. Sci. Pollut. Res. Int. 28, 50565–50578. doi:10.1007/s11356-021-14282-x
Shabir, H., Kundu, S., Basir, S. F., and Khan, L. A. (2014). Amelioration of lead- and Cadmium-Induced Rat Tracheal Hypercontraction by Linalool and Eugenol. Toxicol. Environ. Chem. 96, 307–317. doi:10.1080/02772248.2014.931520
Shakeri, F., Eftekhar, N., Roshan, N. M., Rezaee, R., Moghimi, A., and Boskabady, M. H. (2019). Rosmarinic Acid Affects Immunological and Inflammatory Mediator Levels and Restores Lung Pathological Features in Asthmatic Rats. Allergol. Immunopathol (Madr) 47, 16–23. doi:10.1016/j.aller.2018.04.004
Sharaf, M. H., Abdelaziz, A. M., Kalaba, M. H., Radwan, A. A., and Hashem, A. H. (2021). Antimicrobial, Antioxidant, Cytotoxic Activities and Phytochemical Analysis of Fungal Endophytes Isolated from Ocimum Basilicum. Appl. Biochem. Biotechnol. 1–19. doi:10.1007/s12010-021-03702-w
Siddiqui, B. S., Aslam, H., Begum, S., and Ali, S. T. (2007). New Cinnamic Acid Esters from Ocimum Basilicum. Nat. Prod. Res. 21, 736–741. doi:10.1080/14786410601083639
Siddiqui, B. S., Bhatti, H. A., Begum, S., and Perwaiz, S. (2012). Evaluation of the Antimycobacterium Activity of the Constituents from Ocimum Basilicum against Mycobacterium tuberculosis. J. Ethnopharmacol 144, 220–222. doi:10.1016/j.jep.2012.08.003
Silveira, J. A. d. M., Rocha, D. S., De Morais, G. B., De Siqueira, R. J. B., Leal-Cardoso, J. H., and Evangelista, J. (2017). Inhibitory Effect of Linalool in Preparations of Isolated Smooth Muscle of Rat Trachea with Epithelium Stimulated by Electromechanical Coupling. Ciência Anim. 27, 20–30.
Singh, A. K., Singh, S., Lodhi, S. S., Saxena, S., and Gaur, A. (2011). Essential Oil of Ocimum Basilicum as a Safe Plant Based Antimicrobial in Checking post Harvest Microbial Infestations and Aflatoxin Contamination of Food Commodities. J. Pharm. Res. 4, 526–529.
Singh, S., Majumdar, D. K., and Rehan, H. M. (1996). Evaluation of Anti-inflammatory Potential of Fixed Oil of Ocimum Sanctum (Holybasil) and its Possible Mechanism of Action. J. Ethnopharmacol 54, 19–26. doi:10.1016/0378-8741(96)83992-4
Stanojevic, L. P., Marjanovic-Balaban, Z. R., Kalaba, V. D., Stanojevic, J. S., Cvetkovic, D. J., and Cakic, M. D. (2017). Chemical Composition, Antioxidant and Antimicrobial Activity of Basil (Ocimum Basilicum L.) Essential Oil. J. Essent. Oil Bearing Plants 20, 1557–1569. doi:10.1080/0972060x.2017.1401963
Sundararajan, B., Moola, A. K., Vivek, K., and Kumari, B. D. R. (2018). Formulation of Nanoemulsion from Leaves Essential Oil of Ocimum Basilicum L. And its Antibacterial, Antioxidant and Larvicidal Activities (Culex quinquefasciatus). Microb. Pathog. 125, 475–485. doi:10.1016/j.micpath.2018.10.017
Taghizadeh-Armaki, M., Hedayati, M. T., Moqarabzadeh, V., Ansari, S., Mahdavi Omran, S., Zarrinfar, H., et al. (2017b). Effect of Involved Aspergillus Species on Galactomannan in Bronchoalveolar Lavage of Patients with Invasive Aspergillosis. J. Med. Microbiol. 66, 898–904. doi:10.1099/jmm.0.000512
Taghizadeh-Armaki, M., Hedayati, M. T., Ansari, S., Omran, S. M., Saber, S., Rafati, H., et al. (2017a). Genetic Diversity and In Vitro Antifungal Susceptibility of 200 Clinical and Environmental Aspergillus flavus Isolates. Antimicrob. Agents Chemother. 61, e00004–17. doi:10.1128/aac.00004-17
Tan, P. V., Mezui, C., Enow-Orock, G., Njikam, N., Dimo, T., and Bitolog, P. (2008). Teratogenic Effects, Acute and Sub Chronic Toxicity of the Leaf Aqueous Extract of Ocimum Suave Wild (Lamiaceae) in Rats. J. Ethnopharmacol 115, 232–237. doi:10.1016/j.jep.2007.09.022
Teofilović, B., Grujić-Letić, N., Gligorić, E., Rašković, A., Igić, R., Vastag, G., et al. (2021). Experimental and Computational Evaluation of Extraction Procedure and Scavenging Capacity of Sweet Basil Extracts (Ocimum Basilicum L.). Plant Foods Hum. Nutr. 76 (2), 240–247. doi:10.1007/s11130-021-00902-x
Touiss, I., Ouahhoud, S., Harnafi, M., Khatib, S., Bekkouch, O., Amrani, S., et al. (2021). Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from Ocimum Basilicum L. Evidence-Based Complement. Altern. Med. 2021, 6676998. doi:10.1155/2021/6676998
Tran, T., Nguyen, H., Nguyen, D., Nguyen, T., Tan, H., Nhan, L., et al. (2018). Optimization of Microwave-Assisted Extraction of Essential Oil from Vietnamese Basil (Ocimum Basilicum L.) Using Response Surface Methodology. Processes 6, 206. doi:10.3390/pr6110206
Vardhan, S. (2013). “In Vitro Study of Antioxidant Activity of Ocimum Genus Against Hydrogen Peroxide Induced Oxidative Stress and Apoptosis in Human Lung Fibroblasts,” in In Vitro Cellular & Developmental Biology-Animal (New York, NY: Springer) Vol. 49, S49.
Venâncio, A. M., Onofre, A. S., Lira, A. F., Alves, P. B., Blank, A. F., Antoniolli, A. R., et al. (2011). Chemical Composition, Acute Toxicity, and Antinociceptive Activity of the Essential Oil of a Plant Breeding Cultivar of Basil (Ocimum Basilicum L.). Planta Med. 77, 825–829. doi:10.1055/s-0030-1250607
Vieira, R. F., and Simon, J. E. (2000). Chemical Characterization of Basil (Ocimum spp.) Found in the Markets and Used in Traditional Medicine in Brazil. Econ. Bot. 54, 207–216. doi:10.1007/bf02907824
Yamasaki, K., Nakano, M., Kawahata, T., Mori, H., Otake, T., Ueba, N., et al. (1998). Anti-HIV-1 Activity of Herbs in Labiatae. Biol. Pharm. Bull. 21, 829–833. doi:10.1248/bpb.21.829
Ying, S., Robinson, D. S., Meng, Q., Barata, L. T., Mceuen, A. R., Buckley, M. G., et al. (1999). C-C Chemokines in Allergen-Induced Late-phase Cutaneous Responses in Atopic Subjects: Association of Eotaxin with Early 6-hour Eosinophils, and of Eotaxin-2 and Monocyte Chemoattractant Protein-4 with the Later 24-hour Tissue Eosinophilia, and Relationship to Basophils and Other C-C Chemokines (Monocyte Chemoattractant Protein-3 and RANTES). J. Immunol. 163, 3976–3984.
Youssef, N. A. (2021). Changes in the Morphological Traits and the Essential Oil Content of Sweet Basil (Ocimum Basilicum L.) as Induced by Cadmium and lead Treatments. Int. J. Phytoremediation 23, 291–299. doi:10.1080/15226514.2020.1812508
Zar, H. J., and Ferkol, T. W. (2014). The Global burden of Respiratory Disease-Impact on Child Health. Pediatr. Pulmonol 49, 430–434. doi:10.1002/ppul.23030
Ziaei, M., Sharifi, M., Naghdi Badi, H., Tahsili, J., and Ghorbani Nohooji, M. (2014). A Review on Ocimum Basilicum L. Medicinal Plant with a Focus on the Most Important Secondary Compounds and its Medicinal and Agronomic Properties. J. Med. Plants 4, 26–40.
Złotek, U., Szymanowska, U., Karaś, M., and Świeca, M. (2016). Antioxidative and Anti‐inflammatory Potential of Phenolics from Purple Basil (Ocimum Basilicum L.) Leaves Induced by Jasmonic, Arachidonic and β‐aminobutyric Acid Elicitation. Int. J. Food Sci. Technol. 51, 163–170. doi:10.1111/ijfs.12970
Keywords: Ocimum basilicum L., lung, linalool, respiratory disorders, extract
Citation: Aminian AR, Mohebbati R and Boskabady MH (2022) The Effect of Ocimum basilicum L. and Its Main Ingredients on Respiratory Disorders: An Experimental, Preclinical, and Clinical Review. Front. Pharmacol. 12:805391. doi: 10.3389/fphar.2021.805391
Received: 30 October 2021; Accepted: 03 December 2021;
Published: 03 January 2022.
Edited by:
Alejandro Urzua, University of Santiago, ChileReviewed by:
Ashok K. Shakya, Al-Ahliyya Amman University, JordanRavindra M. Samartha, Bhopal Memorial Hospital and Research Centre, India
Copyright © 2022 Aminian, Mohebbati and Boskabady. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Mohammad Hossein Boskabady, boskabadymh@mums.ac.ir
†These authors share first authorship