AUTHOR=Savassi Bruna , Cordeiro Bárbara F. , Silva Sara H. , Oliveira Emiliano R. , Belo Giovanna , Figueiroa Alessandra Gomes , Alves Queiroz Maria Izabel , Faria Ana Maria Caetano , Alves Juliana , Silva Tales Fernando da , Campos Gabriela Munis , Esmerino Erick A. , Rocha Ramon S. , Freitas Monica Q. , Silva Marcia C. , Cruz Adriano G. , Vital Kátia Duarte , Fernandes Simone O.A. , Cardoso Valbert N. , Acurcio Leonardo Borges , Jan Gwénaël , Le Loir Yves , Gala-Garcia Alfonso , do Carmo Fillipe Luiz R. , Azevedo Vasco TITLE=Lyophilized Symbiotic Mitigates Mucositis Induced by 5-Fluorouracil JOURNAL=Frontiers in Pharmacology VOLUME=12 YEAR=2021 URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.755871 DOI=10.3389/fphar.2021.755871 ISSN=1663-9812 ABSTRACT=

Mucositis is an adverse effect of cancer chemotherapies using 5-Fluorouracil (5-FU). It is characterized by mucosal inflammation, pain, diarrhea, and weight loss. Some studies reported promising healing effects of probiotic strains, when associated with prebiotics, as adjuvant treatment of mucositis. We developed a lyophilized symbiotic product, containing skimmed milk, supplemented with whey protein isolate (WPI) and with fructooligosaccharides (FOS), and fermented by Lactobacillus casei BL23, Lactiplantibacillus plantarum B7, and Lacticaseibacillus rhamnosus B1. In a mice 5-FU mucositis model, this symbiotic lyophilized formulation was able to reduce weight loss and intestinal permeability. This last was determined in vivo by quantifying blood radioactivity after oral administration of 99mTc-DTPA. Finally, histological damages caused by 5-FU-induced mucositis were monitored. Consumption of the symbiotic formulation caused a reduced score of inflammation in the duodenum, ileum, and colon. In addition, it decreased levels of pro-inflammatory cytokines IL-1β, IL-6, IL-17, and TNF-α in the mice ileum. The symbiotic product developed in this work thus represents a promising adjuvant treatment of mucositis.