AUTHOR=Tao Shengchang , Ren Zhiyao , Yang Zerui , Duan Shuna , Wan Zhongxian , Huang Jiahui , Liu Chenxing , Wei Gang 

TITLE=Effects of Different Molecular Weight Polysaccharides From Dendrobium officinale Kimura & Migo on Human Colorectal Cancer and Transcriptome Analysis of Differentially Expressed Genes

JOURNAL=Frontiers in Pharmacology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.704486

DOI=10.3389/fphar.2021.704486

ISSN=1663-9812

ABSTRACT=<p>We investigated the antitumor effects of four fractions of <italic>Dendrobium officinale</italic> Kimura &amp; Migo (<italic>D</italic>. <italic>officinale</italic>) polysaccharides with different molecular weights (Mw), <italic>Astragalus membranaceus</italic> polysaccharides (APS) and <italic>Lentinus edodes</italic> polysaccharides (LNT) on colorectal cancer (CRC) using a zebrafish xenograft model. Transcriptome sequencing was performed to further explore the possible antitumor mechanisms of <italic>D</italic>. <italic>officinale</italic> polysaccharides. Fractions of <italic>D</italic>. <italic>officinale</italic> polysaccharides, LNT, and APS could significantly inhibit the growth of HT-29 cells in a zebrafish xenograft model. One fraction of <italic>D</italic>. <italic>officinale</italic> polysaccharides called DOPW-1 (Mw of 389.98 kDa) exhibited the strongest tumor inhibition. Compared with the control group, RNA-seq revealed that the DOPW-1–treated experimental group had 119 differentially expressed genes (DEGs), of which 45 had upregulated expression and 74 had downregulated expression. Analyses using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes suggested that the pathway “apoptosis-multiple species” was the most significantly enriched. Our data indicated that 1) fractions of <italic>D</italic>. <italic>officinale</italic> polysaccharides of Mw 389.98 kDa were most suitable against CRC; 2) DOPW-1 could be developed into a clinical agent against CRC; and 3) an apoptosis pathway is important for DOPW-1 to inhibit the proliferation of HT-29 cells.</p>