AUTHOR=Wu Xia , Zhao Yan , Gu Ying , Li Kun , Wang Xiaojie , Zhang Jianzhong 

TITLE=Interferon-Lambda 1 Inhibits Staphylococcus aureus Colonization in Human Primary Keratinocytes

JOURNAL=Frontiers in Pharmacology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.652302

DOI=10.3389/fphar.2021.652302

ISSN=1663-9812

ABSTRACT=<p>Atopic dermatitis (AD) is a common inflammatory skin disease. <italic>Staphylococcus aureus</italic> (<italic>S. aureus</italic>) colonization in skin lesions occurs in approximately 70% of AD patients. It has been found that IFN-λ1 can inhibit the colonization of <italic>S. aureus</italic> in normal human nasal mucosa. IFN-λ1 can increase IL-28RA in infected human keratinocytes. In this study, we found that IFN-λ1 can increase mRNA expression of FLG and antimicrobial peptides (AMPs) and inhibit TSLP mRNA expression in infected human keratinocytes. IFN-λ1 can increase intracellular ROS level, decrease STAT1 phosphorylation, and inhibit the colonization of <italic>S. aureus</italic> in human primary keratinocytes. These effects were attenuated by knocking-down IL-28R and NADPH oxidase inhibitor, suggesting that this function was mediated by JAK-STAT1 signaling pathway. These results suggest that IFN-λ1 might have an inhibitory effect on <italic>S. aureus</italic> colonization in AD lesions. Our findings might have potential value in the treatment for AD.</p>