AUTHOR=Zhao Lu , Du Wei , Zhao Dandan , Ji Xueyan , Huang Yanfei , Pang Yong , Guo Kaijin , Yin Xiaoxing 

TITLE=Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function

JOURNAL=Frontiers in Pharmacology

VOLUME=12

YEAR=2021

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2021.626621

DOI=10.3389/fphar.2021.626621

ISSN=1663-9812

ABSTRACT=<p><bold>Objective:</bold> The overall objective of this study was to investigate the effects of catalpol on bone remodeling of diabetic osteoporosis by regulating osteoblast differentiation and migration.</p><p><bold>Method:</bold> Using a murine model of diabetic osteoporosis, to detect the protective effects of catalpol on bone loss, architectural deterioration of trabecular bone and bone metabolism biomarkers were tested. A model of MC3T3-E1 cells was established by treatment with high glucose; the regulatory role of catalpol in the differentiation and migration was tested by Western blot, ALP staining, and Alizarin Red staining.</p><p><bold>Results:</bold> Catalpol treatment markedly ameliorated trabecular bone deterioration by reducing degenerative changes of the trabecular structure by improving the bone formation marker levels of ALP, osteopontin, type I collagen, and osteocalcin, as well as the level of OPG/RANKL. Catalpol enhanced cell motility and scattering following gap formation of MC3T3-E1 cells.</p><p><bold>Conclusion:</bold> The results indicated that catalpol exhibits a protective effect against diabetic osteoporosis by regulating the differentiation and migration of osteoblast.</p>