AUTHOR=Xue Yingxue , Yin Yifei , Li He , Chi Mingyu , Guo Jiaxin , Cui Guihua , Li Wenliang 

TITLE=Synthesis, Anti-Tumor Activity and Apoptosis-Inducing Effect of Novel Dimeric Keggin-Type Phosphotungstate

JOURNAL=Frontiers in Pharmacology

VOLUME=11

YEAR=2021

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.632838

DOI=10.3389/fphar.2020.632838

ISSN=1663-9812

ABSTRACT=<p>A dimeric Keggin-type phosphotungstate (ODA)<sub>10</sub>[(PW<sub>11</sub>FeO<sub>39</sub>)<sub>2</sub>O]·9H<sub>2</sub>O (abbreviated as ODA<sub>10</sub>[(PW<sub>11</sub>Fe)<sub>2</sub>], ODA = octadecyltrimethylammonium bromide) was synthesized and investigated comprehensively its antitumor activity on MCF-7 and A549 cells. The dimeric structure and amorphous morphology were characterized by FT-IR, UV-vis-DRS, SEM and XRD. The <italic>in vitro</italic> MTT assay of ODA<sub>10</sub>[(PW<sub>11</sub>Fe)<sub>2</sub>] showed anticancer activity on MCF-7 and A549 cells in a dose- and time-dependent manner, and the IC<sub>50</sub> values for MCF-7 and A549 cells at 48 h were 5.83 μg/ml and 3.23 μg/ml, respectively. The images of the ODA<sub>10</sub>[(PW<sub>11</sub>Fe)<sub>2</sub>]-treated cells observed by inverted biological microscope exhibited the characteristic morphology of apoptosis. Flow cytometric analysis showed cell apoptosis and cycle arrested at S phase induced by ODA<sub>10</sub>[(PW<sub>11</sub>Fe)<sub>2</sub>]. The above results illuminated the main mechanism of the antitumor action of ODA<sub>10</sub>[(PW<sub>11</sub>Fe)<sub>2</sub>] on MCF-7 and A549 cells, indicating that this dimeric phosphotungstate is a promising anticancer drug.</p>