AUTHOR=Lai Yanni , Zhang Qiong , Long Haishan , Han Tiantian , Li Geng , Zhan Shaofeng , Li Yiwei , Li Zonghui , Jiang Yong , Liu Xiaohong 

TITLE=Ganghuo Kanggan Decoction in Influenza: Integrating Network Pharmacology and In Vivo Pharmacological Evaluation

JOURNAL=Frontiers in Pharmacology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.607027

DOI=10.3389/fphar.2020.607027

ISSN=1663-9812

ABSTRACT=<p><bold>Background:</bold> Ganghuo Kanggan decoction (GHKGD) is a clinical experience prescription used for the treatment of viral pneumonia in the Lingnan area of China, and its clinical effect is remarkable. However, the mechanism of GHKGD in influenza is still unclear.</p><p><bold>Objective:</bold> To predict the active components and signaling pathway of GHKGD and to explore its therapeutic mechanism in influenza and to verified it <italic>in vivo</italic> using network pharmacology.</p><p><bold>Methods:</bold> The potential active components and therapeutic targets of GHKGD in the treatment of influenza were hypothesized through a series of network pharmacological strategies, including compound screening, target prediction and pathway enrichment analysis. Based on the target network and enrichment results, a mouse model of influenza A virus (IAV) infection was established to evaluate the therapeutic effect of GHKGD on influenza and to verify the possible molecular mechanism predicted by network pharmacology.</p><p><bold>Results:</bold> A total of 116 candidate active compounds and 17 potential targets were identified. The results of the potential target enrichment analysis suggested GHKGD may involve the RLR signaling pathway to reduce inflammation in the lungs. <italic>In vivo</italic> experiments showed that GHKGD had a protective effect on pneumonia caused by IAV-infected mice. Compared with the untreated group, the weight loss in the GHKGD group in the BALB/c mice decreased, and the inflammatory pathological changes in lung tissue were reduced (<italic>p</italic> &lt; 0.05). The expression of NP protein and the virus titers in lung were significantly decreased (<italic>p</italic> &lt; 0.05). The protein expression of RIG-I, NF-kB, and STAT1 and the level of MAVS and IRF3/7 mRNA were remarkably inhibited in GHKGD group (<italic>p</italic> &lt; 0.05). After the treatment with GHKGD, the level of Th1 cytokines (IFN-γ, TNF-α, IL-2) was increased, while the expression of Th2 (IL-5, IL4) cytokines was reduced (<italic>p</italic> &lt; 0.05).</p><p><bold>Conclusion:</bold> Through a network pharmacology strategy and <italic>in vivo</italic> experiments, the multi-target and multi-component pharmacological characteristics of GHKGD in the treatment of influenza were revealed, and regulation of the RLR signaling pathway during the anti-influenza process was confirmed. This study provides a theoretical basis for the research and development of new drugs from GHKGD.</p>