AUTHOR=Zhao Yeye , Mei Longfei , Si Yuanqing , Wu Jiadi , Shao Jing , Wang Tianming , Yan Guiming , Wang Changzhong , Wu Daqiang 

TITLE=Sodium New Houttuyfonate Affects Transcriptome and Virulence Factors of Pseudomonas aeruginosa Controlled by Quorum Sensing

JOURNAL=Frontiers in Pharmacology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.572375

DOI=10.3389/fphar.2020.572375

ISSN=1663-9812

ABSTRACT=<p>As a major opportunistic pathogen, <italic>Pseudomonas aeruginosa</italic> can produce various virulence factors and form biofilms. These processes are controlled by the quorum sensing (QS) system. Sodium new houttuyfonate (SNH) is an adduct of houttuyfonate, the main component of the common Chinese medicine plant Houttuynia cordata, which has antibacterial and anti-inflammatory effects. We evaluated the effect of SNH on <italic>P. aeruginosa</italic> biofilms, virulence factors, and transcription. Transcriptome analysis showed that the key <italic>rhlI</italic> and <italic>pqsA</italic> genes of the <italic>P. aeruginosa</italic> QS system were down-regulated after SNH treatment. SNH reduces proteases and pyocyanin production and inhibits biofilm formation by regulating the <italic>P. aeruginosa</italic> QS system. SNH also changes the expression of genes related to virulence factors and biofilms (<italic>lasA, lasB, lecA, phzM, pqsA</italic>, and <italic>pilG</italic>). These results suggested that the mechanism of SNH against <italic>P. aeruginosa</italic> by affecting the expression of biofilm and virulence factors controlled by quorum sensing.</p>