Background

AUTHOR=Li Gang , Woltz Ryan L. , Wang Cheng-yu , Ren Lu , He Pei-xin , Yu Shan-dong , Liu Xue-qin , Yarov-Yarovoy Vladimir , Hu Dan , Chiamvimonvat Nipavan , Wu Lin TITLE=Gating Properties of Mutant Sodium Channels and Responses to Sodium Current Inhibitors Predict Mexiletine-Sensitive Mutations of Long QT Syndrome 3 JOURNAL=Frontiers in Pharmacology VOLUME=11 YEAR=2020 URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.01182 DOI=10.3389/fphar.2020.01182 ISSN=1663-9812 ABSTRACT=Background

Long QT syndrome 3 (LQT3) is caused by SCN5A mutations. Late sodium current (late I_Na) inhibitors are current-specific to treat patients with LQT3, but the mechanisms underlying mexiletine (MEX) -sensitive (N1325S and R1623Q) and -insensitive (M1652R) mutations remains to be elucidated.

Methods

LQT3 patients with causative mutations were treated with oral MEX following i.v. lidocaine. Whole-cell patch-clamp techniques and molecular remodeling were used to determine the mechanisms underlying the sensitivity to MEX.

Results

Intravenous administration of lidocaine followed by MEX orally in LQT patients with N1325S and R1623Q sodium channel mutation shortened QTc interval, abolished arrhythmias, and completely normalized the ECG. In HEK293 cells, the steady-state inactivation curves of the M1652R channels were rightward shifted by 5.6 mV relative to the WT channel. In contrast, the R1623Q mutation caused a leftward shift of the steady-state inactivation curve by 15.2 mV compared with WT channel, and N1325S mutation did not affect steady-state inactivation (n = 5–13, P < 0.05). The extent of the window current was expanded in all three mutant channels compared with WT. All three mutations increased late I_Na with the greatest amplitude in the M1652R channel (n = 9–15, P < 0.05). MEX caused a hyperpolarizing shift of the steady-state inactivation and delayed the recovery of all three mutant channels. Furthermore, it suppressed late I_Na in N1325S and R1623Q to a greater extent compared to that of M1652R mutant channel. Mutations altered the sensitivity of Na_v1.5 to MEX through allosteric mechanisms by changing the conformation of Na_v1.5 to become more or less favorable for MEX binding. Late I_Na inhibitors suppressed late I_Na in N1325S and R1623Q to a greater extent than that in the M1652R mutation (n = 4–7, P < 0.05).

Conclusion

The N1325S, R1623Q, and M1652R mutations are associated with a variable augmentation of late I_Na, which was reversed by MEX. M1652R mutation changes the conformation of Na_v1.5 that disrupt the inactivation of channel affecting MEX binding, corresponding to the poor response to MEX. The lidocaine test, molecular modeling, and drugs screening in cells expressing mutant channels are useful for predicting the effectiveness of late I_Na inhibitors.