AUTHOR=Fan Hua , Wu Qiong , Peng Longping , Li Du , Dong Yidan , Cao Min , Liu Ping , Wang Xu , Hu Xudong , Wang Youhua 

TITLE=Phyllolobium chinense Fisch Flavonoids (PCFF) Suppresses the M1 Polarization of LPS-Stimulated RAW264.7 Macrophages by Inhibiting NF-κB/iNOS Signaling Pathway

JOURNAL=Frontiers in Pharmacology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.00864

DOI=10.3389/fphar.2020.00864

ISSN=1663-9812

ABSTRACT=<sec><title>Background</title><p>M1 macrophage plays an important role in inflammatory reaction. In this study, potential anti-inflammatory effect of <italic>Phyllolobium chinense</italic> Fisch flavonoids (PCFF) was assessed <italic>via</italic> Zebrafish acute inflammation model <italic>in vivo</italic> and LPS-induced pro-inflammatory M1 macrophage model <italic>in vitro</italic>.</p></sec><sec><title>Methods</title><p>The quality control of <italic>P. chinense</italic> Fisch flavonoids (PCFF) was analyzed by HPLC. Anti-inflammatory effect of PCFF on the acute injured zebrafish was evaluated by the migration of fluorescence labeled macrophages and neutrophils, and the gene expression of inflammatory factors. In addition, the anti-inflammatory mechanism of PCFF was investigated by the related gene expression and related signaling pathway regulation of pro-inflammatory mediators in LPS-induced pro-inflammatory M1 RAW264.7 macrophage.</p></sec><sec><title>Results</title><p><italic>P. chinense</italic> Fisch flavonoids (PCFF) markedly suppressed macrophage and neutrophil migration and iNOS gene expression in acute injured zebrafish with tail-cutting. PCFF significantly inhibited NO overproduction and iNOS gene overexpression in LPS-sitimulated pro-inflammatory M1 RAW264.7 macrophages. What’s more, PCFF could evidently decrease p65 protein production, but had no effect on the production of P38, JNK and ERK1/2 proteins.</p></sec><sec><title>Conclusion</title><p><italic>P. chinense</italic> Fisch flavonoids (PCFF) have a remarkable inhibitory effect on the inflammatory response in acute injured zebrafish and LPS-stimulated M1 RAW264.7 macrophage. The pharmacological mechanism may be related to the regulation of NO overproduction and the inhibition of NF-κB/iNOS signaling pathway.</p></sec>