AUTHOR=Yao Yuebao , Mi Wenjuan , Cao Guangzhao , Yang Ruiqi , Chen Huirong , Liu Yong , Zou Huiqin , Yan Yonghong 

TITLE=The Absorption Characteristics of Nonvolatile Components in a Water Extraction From Amomi fructus as Determined by In Situ Single-Pass Intestinal Perfusion and High-Performance Liquid Chromatography

JOURNAL=Frontiers in Pharmacology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.00711

DOI=10.3389/fphar.2020.00711

ISSN=1663-9812

ABSTRACT=<sec><title>Background</title><p><italic>Amomi fructus</italic> is a famous traditional Chinese medicine (TCM) that can exert beneficial effects during the treatment of gastrointestinal diseases and is used widely in China and other countries in Southeast Asia. However, the nonvolatile active ingredients that are present in the water extractions from <italic>A. fructus</italic> used to treat gastrointestinal diseases have yet to be elucidated. The goal of this study was to identify the nonvolatile active ingredients of <italic>A. fructus</italic>.</p></sec><sec><title>Methods</title><p>We used an <italic>in situ</italic> single-pass intestinal perfusion (SPIP) model to identify the active ingredients of <italic>A. fructus</italic> that play significant roles in gastrointestinal absorption. In addition, we developed a high-performance liquid chromatography (HPLC) method to identify key fractions in intestinal outflow perfusate.</p></sec><sec><title>Results</title><p>Nineteen components were identified in a water extraction from <italic>A. fructus</italic>; these exhibited different absorption capabilities in different intestinal segments. Of these, six components were determined by the newly developed HPLC method: catechin, vanillic acid, epicatechin, polydatin, isoquercitrin, and quercitrin.</p></sec><sec><title>Conclusions</title><p>The current study aimed to identify the active ingredients present in water extractions prepared from <italic>A. fructus</italic> in a single-intestinal perfusate from rats. Our findings provide an experimental basis to explain the pharmacodynamic actions of <italic>A. fructus</italic>.</p></sec>