AUTHOR=Wang Shang , Yu Lie , Sun Guifang , Liu Yu , Hu Wentao , Liu Yanru , Peng Tao , Wang Xiaojun , Cheng Jingliang , Sr Aravintakumar , Qin Bo , Lu Hong 

TITLE=Danhong Injection Protects Hemorrhagic Brain by Increasing Peroxiredoxin 1 in Aged Rats

JOURNAL=Frontiers in Pharmacology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.00346

DOI=10.3389/fphar.2020.00346

ISSN=1663-9812

ABSTRACT=<p>Intracerebral hemorrhage (ICH) is a severe cerebrovascular disease with a high incidence, mortality and disability rate. Danhong injection (DHI) is beneficial for ischemic stroke, but is prohibited for ICH due to risk of bleeding. The present study aims to explore the potential therapeutic time window and molecular mechanism of DHI in a collagenase-induced ICH model in aged rats. DHI administration after ICH could significantly improve body weight and neurological deficits, and reduce the hematoma volume and brain water content when compared to the vehicle control. Furthermore, the protective effect of DHI administration on days 1–3 after ICH was superior to those on days 3–5 or 7–9 after ICH. DHI remarkably increased the <italic>Peroxiredoxin 1</italic> (<italic>Prx1</italic>) expression in astrocytes and reduced the expression of inflammatory factors tumor necrosis factor-α (TNF-α) and interleukin-β (IL-1β) after ICH. The immediate treatment of <italic>Prx1</italic> inhibiter chelerythrine (Che) after ICH abolished the protective effect of DHI. Furthermore, the Che treatment reduced the expression of <italic>Prx1</italic> in astrocytes, but increased the expression of TNF-α and IL-1β after ICH. DHI treatment could not reverse these changes. Therefore, the earlier DHI is administered, the better the neuroprotective effect. DHI exerts antioxidative and anti-inflammatory function by increasing <italic>Prx1</italic> in astrocytes. These present results may change the established understanding of DHI, and reveal a novel treatment approach for ICH.</p>