AUTHOR=Bi Decheng , Li Xiaofan , Li Tong , Li Xiuting , Lin Zhijian , Yao Lijun , Li Hui , Xu Hong , Hu Zhangli , Zhang Zhenqing , Liu Qiong , Xu Xu 

TITLE=Characterization and Neuroprotection Potential of Seleno-Polymannuronate

JOURNAL=Frontiers in Pharmacology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2020.00021

DOI=10.3389/fphar.2020.00021

ISSN=1663-9812

ABSTRACT=<p>Seleno-polymannuronate (Se-PM) was prepared from alginate-derived polymannuronate (PM) through a sulfation followed by a selenylation replacement reaction. The organic selenium content of Se-PM was 437.25 μg/g and its average molecular weight was 2.36 kDa. The neuroprotection effect of Se-PM and corresponding molecular mechanisms were investigated. Our results showed that, comparing to both sulfated PM (S-PM) and PM, Se-PM remarkably inhibited the aggregation of Aβ<sub>1–42</sub> oligomer <italic>in vitro</italic> and significantly reduced the APP and BACE1 protein expression in N2a-sw cells, highlighting the critical function of the selenium presented in Se-PM. Moreover, Se-PM decreased the expression of cytochrome c and the ratio of Bax to Bcl-2, and enhanced the mitochondrial membrane potential in N2a-sw cells. These results suggested that Se-PM treatment can markedly inhibit N2a-sw cell apoptosis and promote N2a-sw cell survival and that Se-PM might be a potential therapeutic agent for the prevention of neurodegeneration owing to its remarkable neuroprotection effect.</p>