Teresa Giani1,2*
Salvatore De Masi3
Ilaria Maccora4
Francesca Tirelli4Gabriele Simonini4Martina Falconi5Rolando Cimaz1,6- 1Rheumatology Unit, A. Meyer Children’s Hospital, Florence, Italy
- 2Department of Medical Biotechnology, University of Siena, Siena, Italy
- 3Clinical Trial Office, A. Meyer Children’s Hospital, Florence, Italy
- 4Rheumatology Unit, A. Meyer Children’s Hospital, University of Florence, Florence, Italy
- 5Technical-Scientific Secretariat of the Paediatric Regional Ethics Committee, Florence, Italy
- 6Department of Clinical Sciences and Community Health, University of Milan, Milan, Italy
Background: There is evidence that obesity could be a risk factor for the severity and response to treatment in adult patients with rheumatoid arthritis (RA) due both to the mechanical effect of overweight and to the potential pro-inflammatory effects of cytokines produced by adipose tissue.
Objectives: To evaluate the role of overweight and obesity in a cohort of young patients with juvenile idiopathic arthritis (JIA) in terms of incidence, disease activity, outcome, and response to treatments.
Methods: This single-center retrospective cohort study evaluated 110 children affected by JIA under treatment with conventional disease-modifying antirheumatic drugs (DMARDs) and biologic agents. Body mass index (BMI) categories of 5–84th (normal weight), 85–94th (overweight), and ≥95th (obese) percentile were used. Patients with systemic JIA, uveitis, chronic comorbidities, or under other potentially confounding systemic treatments were excluded. Uni- and multivariate analyses were performed.
Results: One hundred and ten JIA patients (polyarticular n = 50, oligoarticular n = 38, psoriatic n = 12, enthesitis-related arthritis n = 8, undifferentiated n = 2) were enrolled in the study, 75% girls and 25% boys. The mean age at treatment onset was 6.09 years. Baseline BMI was ≥5th and ≤84th percentile in 80 patients, 85–94th in 27, and ≥95th in 3.
We did not observe a significant association between BMI and erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), or number of active joints at baseline, while involvement of the joints of lower limbs was significantly greater ( p = 0.025) in overweight/obese patients. However, a trend toward lower remission rates and higher number of relapses, both after DMARDs and biologics, in patients with higher BMI was observed.
Conclusion: This study focuses on the relationship between overweight/obesity and JIA. A significant correlation between obesity and a greater involvement of the joints of the lower limbs at baseline was demonstrated. Furthermore, our data suggest that obesity could negatively influence the course of the disease as well as treatment response.
Introduction
Obesity is a medical condition characterized by an excess in body fat accumulation associated with a potentially negative impact on health (Fact Sheet on Obesity and Overweight, 2006). Body mass index (BMI, weight/height2, kg/m2) is the most widely used method to define obesity and overweight, although it does not take into account body composition and the share of adiposity (Cole et al., 2000). Referring to the US Centers for Disease Control and Prevention (CDC) BMI-for-age growth chart, obesity is defined among subjects aged 2 to 19 years in the presence of a BMI at or above the sex-specific 95th percentile, while overweight is defined as a BMI at or above the 85th percentile and below the 95th (Krebs et al., 2007; Katzmarzyk et al., 2015).
The prevalence of overweight and obesity in European countries accounts for 53.1%, and in the pediatric age, it is about 20%, ranging from 40% in Southern Europe to less than 10% in Northern European countries (Ahrens et al., 2014; Marques et al., 2018).
An increased accumulation of body fat represents a significant risk factor for metabolic complications and a modifiable variable for a number of chronic diseases (Finucane et al., 2011). A wealth of studies both in mice and in humans have shown increased levels of pro-inflammatory cytokines in adipose tissues, including TNF-α, interleukin 6 (IL-6), IL-1β, and several other inflammatory proteins (Hotamisligil et al., 1995; Berg and Scherer, 2005).
Recently, evidence has emerged in favor of the potential negative influence of obesity on rheumatoid arthritis (RA) disease progression, disease severity, and treatment response (Klaasen et al., 2011; Gremese et al., 2013; Heimans et al., 2013; Ellerby et al., 2014; Sandberg et al., 2014; Liu et al., 2017). Liu et al. (2017) in their meta-analysis documented the unfavorable effect of obesity on achieving remission in RA and its impact on disease activity and patient-reported outcomes during therapy. Conversely, only few data have been published about the effect of obesity on juvenile idiopathic arthritis (JIA).
Thus, the goal of our study was to investigate the relationship between BMI and JIA in terms of baseline disease activity and response to classic disease-modifying antirheumatic drugs (DMARDs) and biological agents.
Materials and Methods
A retrospective study was conducted with an analysis of the medical records of children with JIA classified according to International League of Associations for Rheumatology (ILAR) criteria that were seen in the Pediatric Rheumatology Unit of Anna Meyer Children Hospital between January 2009 and January 2017 (Petty et al., 2004). Only patients who needed systemic drugs were selected. Arthritis related with uveitis and systemic JIA subtypes were considered exclusion criteria.
Demographic data, age at disease onset, anthropometric measurements, disease duration, laboratory data, joint involvement, and medications including DMARDs and biologic drugs were extracted from charts and inserted into a dedicated database. Patients were usually evaluated every 3 months with a thorough physical examination inclusive of assessment of the number and the type of active joints (swelling or both tenderness and limited range of motion) and laboratory investigations.
Height was measured with a wall-mounted stadiometer to the nearest 0.1 cm, while for weight, a calibrated beam balance platform scale to the nearest 0.1 kg was used. BMI was calculated as weight in kilograms divided by height in square meters. BMI-for-age percentiles were computed using the CDC reference values. Normal BMI was defined as the 5th percentile to less than the 85th percentile, overweight as the 85th to less than the 95th percentile, and obese as equal to or greater than the 95th percentile (Kuczmarski et al., 2002).
Relevant laboratory variables included erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP). Clinically inactive disease status was defined as no joints with active disease, an ESR of ≤20 mm/h, and CRP ≤0.5 mg/dl.
The data were analyzed using the person time method. Patients on treatment with first-line drugs (DMARDs) were followed from the start of treatment up to the date of remission or last follow-up. The remission rates for person-months were therefore calculated. The rates of relapse were calculated as the number of relapse (first occurrence) after remission on person-months at risk. The amount of person-months was the sum of months at risk from the date of remission to the date of relapse or of the last follow-up. For subjects eligible for treatment with biological drugs, remission and relapse post-remission rates were calculated using the same method. We performed univariate and multivariate analysis and included in the models clinically and statistically relevant variables as covariate. All analyses were performed with the STATA 11 software.
Since we used deidentified data, according to national regulation, no ethics committee approval or informed consent was needed.
Results
Demographic and clinical data are reported in Table 1. One hundred and ten JIA patients (polyarticular n = 50, oligoarticular n = 38, psoriatic n = 12, enthesitis-related arthritis n = 8, undifferentiated n = 2) were enrolled in the study, 75% girls and 25% boys. The mean age at conventional DMARDs treatment onset was 6.09 years. Baseline BMI was included between the 5th and 84th percentile in 80 patients, 85–94th in 27, and ≥95th in 3.
Table 1 Baseline characteristics of the population.
At baseline, ESR and CRP values were increased, although not significantly, in overweight/obese compared with healthy weighted individuals (p = 0.373 and 0.176 for ESR and CRP, respectively). The number of active lower limb joints was significantly lower (p = 0.028) in healthy weighted than in overweight/obese individuals (Table 1).
Sixty-nine patients out of 110 achieved remission on conventional DMARDs. The remission rate was 93.88/1000 person-months, with higher remission rates in healthy weighted children (98.87) than in obese children (83.58). At univariate analysis, however, the difference did not reach statistical significance. The hazard ratio (HR) in obese children compared with healthy weighted children was 0.8, CI 95% 0.46–1.40. An abnormal CRP value was the only covariate influencing the remission rate, close to statistical significance (HR 1.79, CI 95% 0.98–3.26) (Table 2).
Table 2 Remission with classical DMARDs.
The multivariate analysis showed a significant effect of elevated CRP value on the remission rate (HR 2.21, CI 95% 1.12–4.35). Lower remission rates were observed in children with an overweight/obese BMI and a higher number of active joints (more than five) at baseline as well as in patients with increased values of ESR and CRP compared to normal values (HR 1.77, CI 95% 0.62–5.06 and HR 1.72, CI 95% 0.75–3.98, for ESR and CRP, respectively), although without a statistical significance (Tables 3 and 4). Thirty-five patients out of 69 relapsed after remission, with a relapse rate of 13.40/1000 person-months. The relapse rate was slightly higher in obese children (15.79) than in healthy weighted children (13.37). The HR was 1.25, CI 95% 0.6–2.6.
Table 3 Remission with classic (DMARDs).
Table 4 Relapse post-remission with classic DMARDs.
The Cox regression showed a higher HR of relapse rate in overweight/obese children compared to healthy weighted children, and in children with abnormal values of CRP compared to those with normal values. However, all HR estimates did not reach statistical significance (Table 5).
Table 5 Relapse post-remission with classic DMARDS.
Forty-eight patients required a second-line treatment: 27 etanercept (13 in overweight/obese), 19 adalimumab (5 in overweight/obese), and 2 abatacept (all in healthy weighted children).
Remission rate with second-line therapy was 122.12/1,000 person-months, higher in healthy weighted children (146.21) than in obese children (87.45). The HR was 0.61, CI 95% 0.31–1.20.
Remission rates were lower in individuals with several active joints when compared with those with few active joints (>4 vs. 0–4). The corresponding HR were 0.59, CI 95% 0.32–1.08 for more than 4 active joints vs. 0–4, and 0.46, CI 95% 0.19–1.13 (>4 vs. 0–4 joints) considering only lower limbs (Table 6). The multivariate analysis confirmed the results of the univariate calculation (Table 7).
Table 6 Remission with second-line therapy (biologics).
Table 7 Remission with second-line therapy (biologics).
Discussion
We investigated the negative effect exerted by obesity on JIA as suggested by data on adults affected by RA. Although JIA shares some features with RA, they should be considered distinct diseases. JIA represents the most common rheumatic disease of childhood with a prevalence of 16–150 cases per 100,000 children (Prakken et al., 2011). Regardless of the differences in the underlying pathogenesis of the various types of JIA, the immunopathology involves a predominant abnormality of the adaptive immune system with a consistent overproduction of pro-inflammatory cytokines, many of which, such as TNF-α, IL-1, and IL-6, have been shown to be increased also in the presence of adipose expansion.
We observed a very small number of obese subjects in our cohort of patients; therefore, we divided it into two groups, one comprising children with a “healthy weight” (BMI < 85th percentile) and the other comprising children with an “increased weight” (BMI ≥ 85th percentile). Overweight/obesity rate in our study population is 27%, reflecting the pediatric prevalence of this condition in our country. We also excluded patients affected by systemic subtype, for its clinical heterogeneity compared to the other JIA subtypes, and patients with uveitis, which may require further treatments despite a good arthritis control, to avoid confounding factors.
At baseline, we observed a negative influence of obesity/overweight on disease activity. Our JIA overweight/obese children showed a tendency to have a greater number of active joints, in particular those of lower limbs, and higher values of ESR and CRP compared to healthy weight subjects.
Few data are available about the impact of obesity on JIA. A first study by Pelajo et al. analyzed 154 JIA subjects, 18% of whom were obese and 12% were overweight; no association was found between obesity and disease activity in terms of Juvenile Arthritis Disease Activity Score 27, physician’s assessment of disease activity, parent’s assessment of child’s well-being, CRP, ESR, and number of active joints (Pelajo et al., 2012).
On the other hand, Amine et al. (2011) in their series of 58 Moroccan JIA patients, characterized by high BMI prevalence (41.4% overweight and 22.4% obese), observed a correlation between overweight and functional limitation and severe joint pain.
In 2016, Makay et al. studied 72 patients with enthesitis-related arthritis, 27% of whom have a BMI ≥ 85th percentile. In subjects with increased BMI, they observed a higher frequency of tarsitis and ankle arthritis at baseline, while sacroiliac, hip, and entheses involvement as well as ESR values were similar to those with a BMI < 85th percentile (Makay et al., 2016).
Compared to adult RA, the correlation between disease activity and obesity seems to be less evident in JIA. Furthermore, data regarding the influence of BMI in treatment responses and outcome are still very poor in the pediatric age. In RA, obesity appears to be a strong predictor of worse treatment responses, decreasing the chances of achieving good disease control during the early phase, in which most of the patients receive DMARDs (Sandberg et al., 2014). Some recent studies have shown that the effect of TNF blockage also appears to be impaired in obese RA patients, while no significant difference between obese and non-obese were seen in patients treated with abatacept and tocilizumab (Klaasen et al., 2011; Gremese et al., 2013; Iannone et al., 2015; Kim et al., 2016; Shan and Zhang, 2018). The modification in the pharmacokinetics and pharmacodynamics of drugs related to the excess of fat has been hypothesized to influence the response to therapy. On the other hand, obesity may mask early identification of active joints, delaying the beginning of the correct treatment (Barnabe et al., 2014).
In pediatrics, only the study by Makay et al. (2016) evaluated the response to therapy in patients with enthesitis-related arthritis with different BMI, reporting a failure in achieving a clinically inactive disease in children with a higher BMI treated with DMARDs or anti-TNF drugs, suggesting a negative effect of obesity on disease outcome.
In our JIA population, obesity/overweight corresponds to a worse trend in the therapeutic response both to first-line treatments and to anti-TNF drugs, although without reaching statistical significance.
Despite the fact that our data do not identify obesity as an independent risk factor for JIA, they suggest a negative influence on disease activity at baseline and an unfavorable impact on the therapeutic response to conventional DMADs and anti-TNF drugs. These observations may take into consideration weight management with dietary and physical activity interventions as part of the treatment strategy for obese/overweight patients.
Limitations of our study include the fact that few of our patients were obese and that the sample size of our population was relatively small; this may have hampered the achievement of statistical significance of several comparisons. Nonetheless, our study is one of the few that have investigated such factors and deserves further investigations.
Author Contributions
TG was in charge of project planning and paper writing. FT and IM were in charge of data collection. SM and MF performed the statistical analysis. GS and RC were the coordinators.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Acknowledgments
We acknowledge that part of this work has been previously published as a conference proceeding abstract (Pediatric Rheumatology 2018;16 (Suppl 2):52).
References
Ahrens, W., Pigeot, I., Pohlabeln, H., De Henauw, S., Lissner, L., Molnár, D., et al. (2014). Prevalence of overweight and obesity in European children below the age of 10. Int. J. Obes. (Lond) 38Suppl2, S99–S107. doi: 10.1038/ijo.2014.140
Amine, B., Ibn Yacoub, Y., Rostom, S., Hajjaj-Hassouni, N. (2011). Prevalence of overweight among Moroccan children and adolescents with juvenile idiopathic arthritis. Joint Bone Spine 78 (6), 584–586. doi: 10.1016/j.jbspin.2011.02.001
Barnabe, C., Xiong, J., Pope, J. E., Boire, G., Hitchon, C., Haraoui, B., et al. (2014). Factors associated with time to diagnosis in early rheumatoid arthritis. Rheumatol. Int. 34 (1), 85–92. doi: 10.1007/s00296-013-2846-5
Berg, A. H., Scherer, P. E. (2005). Adipose tissue, inflammation, and cardiovascular disease. Circ. Res. 96 (9), 939–949. doi: 10.1161/01.RES.0000163635.62927.34
Cole, T. J., Bellizzi, M. C., Flegal, K. M., Dietz, W. H. (2000). Establishing a standard definition for child overweight and obesity worldwide: international survey. WH BMJ 320 (7244), 1240–1243. doi: 10.1136/bmj.320.7244.1240
Ellerby, N., Mattey, D. L., Packham, J., Dawes, P., Hider, S. L. (2014). Obesity and comorbidity are independently associated with a failure to achieve remission in patients with established rheumatoid arthritis. Ann. Rheum. Dis. 73 (11), e74. doi: 10.1136/annrheumdis-2014-206254
Fact Sheet on Obesity and Overweight. World Health Organization Media Center, Geneva, Switzerland (2006) http://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight.
Finucane, M. M., Stevens, G. A., Cowan, M. J., Danaei, G., Lin, J. K., Paciorek, C. J., et al. (2011). National, regional, and global trends in body-mass index since 1980: systematic analysis of health examination surveys and epidemiological studies with 960 country-years and 9.1 million participants. Lancet 377 (9765), 557–567. doi: 10.1016/S0140-6736(10)62037-5
Gremese, E., Carletto, A., Padovan, M., Atzeni, F., Raffeiner, B., Giardina, A. R., et al. (2013). Obesity and reduction of the response rate to anti-tumor necrosis factor α in rheumatoid arthritis: an approach to a personalized medicine. Arthritis Care Res. (Hoboken) 65 (1), 94–100. doi: 10.1002/acr.21768
Heimans, L., van den Broek, M., le Cessie, S., Siegerink, B., Riyazi, N., Han, K. H., et al. (2013). Association of high body mass index with decreased treatment response to combination therapy in recent-onset rheumatoid arthritis patients. Arthritis Care Res. (Hoboken) 65 (8), 1235–1242. doi: 10.1002/acr.21978
Hotamisligil, G. S., Arner, P., Caro, J. F., Atkinson, R. L., Spiegelman, B. M. (1995). Increased adipose tissue expression of tumor necrosis factor-alpha in human obesity and insulin resistance. J. Clin. Invest. 95 (5), 2409–2415. doi: 10.1172/JCI117936
Iannone, F., Fanizzi, R., Notarnicola, A., Scioscia, C., Anelli, M. G., Lapadula, G. (2015). Obesity reduces the drug survival of second line biological drugs following a first TNF-α inhibitor in rheumatoid arthritis patients. Joint Bone Spine 82 (3), 187–191. doi: 10.1016/j.jbspin.2014.12.006
Katzmarzyk, P. T., Barreira, T. V., Broyles, S. T., Chaput, J. P., Fogelholm, M., Hu, G., et al. (2015). Association between body mass index and body fat in 9–11-year-old children from countries spanning a range of human development. Int. J. Obes. Suppl. 5 (Suppl 2), S43–S46. doi: 10.1038/ijosup.2015.18
Kim, S. K., Choe, J. Y., Park, S. H., Lee, H. (2016). No predictive effect of body mass index on clinical response in patients with rheumatoid arthritis after 24 weeks of biological disease-modifying antirheumatic drugs: a single-center study. Clin. Rheumatol. 35 (5), 1129–1136. doi: 10.1007/s10067-016-3220-2
Klaasen, R., Wijbrandts, C. A., Gerlag, D. M., Tak, P. P. (2011). Body mass index and clinical response to infliximab in rheumatoid arthritis. Arthritis Rheum. 63 (2), 359–364. doi: 10.1002/art.30136
Krebs, N. F., Himes, J. H., Jacobson, D., Nicklas, T. A., Guilday, P., Styne, D. (2007). Assessment of child and adolescent overweight and obesity. Pediatrics 120 Suppl;4, S193–S228. doi: 10.1542/peds.2007-2329D
Kuczmarski, R. J., Ogden, C. L., Guo, S. S., Grummer-Strawn, L. M., Flegal, K. M., Mei, Z., et al. (2002). 2000 CDC growth charts for the United States: methods and development. Vital Health Stat. 11 246, 1–190.
Liu, Y., Hazlewood, G. S., Kaplan, G. G., Eksteen, B., Barnabe, C. (2017). Impact of obesity on remission and disease activity in rheumatoid arthritis: a systematic review and meta-analysis. Arthritis Care Res. (Hoboken) 69 (2), 157–165. doi: 10.1002/acr.22932
Makay, B., Gücenmez, ÖA, Ünsal, E. (2016). Inactive disease in enthesitis-related arthritis: Association of increased body mass index. J. Rheumatol. 43 (5), 937–943. doi: 10.3899/jrheum.151208
Marques, A., Peralta, M., Naia, A., Loureiro, N., de Matos, M. G. (2018). Prevalence of adult overweight and obesity in 20 European countries, 2014. Eur. J. Public Health 28 (2), 295–300. doi: 10.1093/eurpub/ckx143
Pelajo, C. F., Lopez-Benitez, J. M., Miller, L. C. (2012). Obesity and disease activity in juvenile idiopathic arthritis. Pediatr. Rheumatol. Online J. 10 (1), 3. doi: 10.1186/1546-0096-10-3
Petty, R. E., Southwood, T. R., Manners, P., Baum, J., Glass, D. N., Goldenberg, J., et al. (2004). International League of Associations for Rheumatology classification of juvenile idiopathic arthritis: second revision, Edmonton, 2001. J. Rheumatol. 31 (2), 390–392.
Prakken, B., Albani, S., Martini, A. (2011). Juvenile idiopathic arthritis. Lancet 377 (9783), 2138–2149. doi: 10.1016/S0140-6736(11)60244-4
Sandberg, M. E., Bengtsson, C., Källberg, H., Wesley, A., Klareskog, L., Alfredsson, L., et al. (2014). Overweight decreases the chance of achieving good response and low disease activity in early rheumatoid arthritis. Ann. Rheum. Dis. 73 (11), 2029–2033. doi: 10.1136/annrheumdis-2013-205094
Keywords: obesity, overweight, body mass index, juvenile idiopathic arthritis, disease-modifying antirheumatic drugs, biologics
Citation: Giani T, De Masi S, Maccora I, Tirelli F, Simonini G, Falconi M and Cimaz R (2019) The Influence of Overweight and Obesity on Treatment Response in Juvenile Idiopathic Arthritis. Front. Pharmacol. 10:637. doi: 10.3389/fphar.2019.00637
Received: 14 December 2018; Accepted: 17 May 2019;
Published: 12 June 2019.
Edited by:
Cecilia Beatrice Chighizola, Istituto Auxologico Italiano (IRCCS), ItalyReviewed by:
Micol Romano, Istituto Ortopedico Gaetano Pini, ItalySilvia Magni Manzoni, Bambino Gesù Ospedale Pediatrico, Italy
Copyright © 2019 Giani, De Masi, Maccora, Tirelli, Simonini, Falconi and Cimaz. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Teresa Giani, dGVyZXNhLmdpYW5pQGdtYWlsLmNvbQ==