AUTHOR=Huang Yuqin , Zhou Quan , Wang Wenguo , Huang Qiang , Liao Juan , Li Junyi , Long Lei , Ju Tao , Zhang Quan , Wang Hanqin , Xu Huaqiang , Tu Mingli 

TITLE=Acinetobacter baumannii Ventilator-Associated Pneumonia: Clinical Efficacy of Combined Antimicrobial Therapy and in vitro Drug Sensitivity Test Results

JOURNAL=Frontiers in Pharmacology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2019.00092

DOI=10.3389/fphar.2019.00092

ISSN=1663-9812

ABSTRACT=<p><bold>Objective:</bold> To evaluate therapeutic efficacy of different combined antimicrobial treatments against <italic>Acinetobacter baumannii</italic> ventilator-associated pneumonia (VAP).</p><p><bold>Methods:</bold> Clinical outcomes were retrospectively analyzed to elucidate the efficacy of four combined antimicrobial regimens. The chessboard and micro broth dilution methods determined the minimum inhibitory concentrations (MICs) of four antiseptic drugs singly used and combined two drugs against 36 isolates of multidrug-resistant (MDR) <italic>A. baumannii</italic>.</p><p><bold>Results:</bold> The incidence of VAP was approximately 6.9% (237/3424) between January 1, 2015 and December 31, and 35.9% (85/237) of the cases were caused by <italic>A. baumannii</italic>. Among these cases, 60 belonged to AB-VAP, for whom antimicrobial treatment plan was centralized and clinical data was complete. Moreover, all 60 strains of <italic>A. baumannii</italic> were MDR bacteria from reports microbiological laboratory. Resistance rate was lowest for amikacin (68.3%) and ampicillin sulbactam (71.7%). Resistance rate for imipenem increased from 63.2 to 90.9% during the 3 years. However, in these 60 cases of AB-VAP, the combination between 4 antibiotics was effective in most cases: the effective rate was 75% (18/24) for sulbactam combined with etilmicin, 71.4% (10/14) for sulbactam combined with levofloxacin, 72.7% (8/11) for meropenem combined with etilmicin, and 63.6% (7/11) for meropenem combined with levofloxacin. There was no statistical difference between four regimens (<italic>P</italic> &gt; 0.05). Sulbactam combined with etilmicin decreased 1/2 of MIC<sub>50</sub> and MIC<sub>90</sub> of sulbactam while the decreases in etilmicin were more obviously than single drug. When adopting meropenem combined with levofloxacin or etilmicin, the MIC of meropenem reduced to 1/2 of that in applying single drug. As for sulbactam or meropenem combined with levofloxacin, it also lessened the MIC<sub>50</sub> of levofloxacin to 1/2 of that for single drug. FIC results suggested that the effects of four combined antimicrobial regimens were additive or unrelated. When sulbactam was combined with etimicin, the additive effect was 63.89%.</p><p><bold>Conclusion:</bold> Drug combination sensitivity test <italic>in vitro</italic> may be helpful for choosing antimicrobial treatment plans. Sulbactam or meropenem as the basis of treatment regimens can function as the alternatives against AB-VAP. Sulbactam combined with etimicin has been regarded as a recommended regimen in Suizhou, Hubei, China.</p>