AUTHOR=Boakye Yaw D. , Agyare Christian , Ayande George P. , Titiloye Nicholas , Asiamah Emmanuel A. , Danquah Kwabena O. 

TITLE=Assessment of Wound-Healing Properties of Medicinal Plants: The Case of Phyllanthus muellerianus

JOURNAL=Frontiers in Pharmacology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00945

DOI=10.3389/fphar.2018.00945

ISSN=1663-9812

ABSTRACT=<p><italic>Phyllanthus muellerianus</italic> (Family Euphorbiaceae) is a shrub, which is widely distributed in West Africa and employed traditionally as a wound-healing agent especially in Ghana. The aim of the study was to determine the <italic>in vivo</italic> wound-healing activity of aqueous aerial part extract of <italic>P. muellerianus</italic> (PLE) and its major isolate, geraniin. Excision and incision wound models were used to determine the wound-healing activity. Wounds were treated with PLE (0.25, 0.5, and 1% w/w) and geraniin (0.1, 0.2, and 0.4% w/w) aqueous creams. PLE and geraniin significantly (<italic>p</italic> &lt; 0.001) increased wound contraction rate and hydroxyproline production compared to untreated wounds. Histological studies of wound tissues showed high levels of fibroblasts and increased collagen content and cross-linking in PLE and geraniin-treated wound tissues. Immuno-histochemical investigations revealed high levels of TGF-β<sub>1</sub> in PLE and geraniin-treated wound tissues compared to the untreated wound tissues. Tensile strength of incised wounds was significantly (<italic>p</italic> &lt; 0.05) high in PLE and geraniin-treated wounds. PLE (0.1–100 μg/mL) significantly (<italic>p</italic> &lt; 0.001) reduce LDH release from HaCaT-keratinocytes compared to the untreated cells. PLE and geraniin possess wound healing and cytoprotective effect.</p>