AUTHOR=Dangi Arun K. , Sinha Rajeshwari , Dwivedi Shailja , Gupta Sanjeev K. , Shukla Pratyoosh 

TITLE=Cell Line Techniques and Gene Editing Tools for Antibody Production: A Review

JOURNAL=Frontiers in Pharmacology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00630

DOI=10.3389/fphar.2018.00630

ISSN=1663-9812

ABSTRACT=<p>The present day modern formulation practices for drugs are based on newer tools and techniques toward effective utilization. The methods of antibody formulations are to be revolutionized based on techniques of cell engineering and gene editing. In the present review, we have discussed innovations in cell engineering toward production of novel antibodies for therapeutic applications. Moreover, this review deciphers the use of RNAi, ribozyme engineering, CRISPR-Cas-based techniques for better strategies for antibody production. Overall, this review describes the multidisciplinary aspects of the production of therapeutic proteins that has gained more attention due to its increasing demand.</p>