AUTHOR=Kang Myung-Ji , Kwon Eun-Bin , Ryu Hyung Won , Lee Seoghyun , Lee Jae-Won , Kim Doo-Young , Lee Mi Kyeong , Oh Sei-Ryang , Lee Hyun-Sun , Lee Su Ui , Kim Mun-Ock 

TITLE=Polyacetylene From Dendropanax morbifera Alleviates Diet-Induced Obesity and Hepatic Steatosis by Activating AMPK Signaling Pathway

JOURNAL=Frontiers in Pharmacology

VOLUME=9

YEAR=2018

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2018.00537

DOI=10.3389/fphar.2018.00537

ISSN=1663-9812

ABSTRACT=<p>The extract tea of <italic>Dendropanax morbifera</italic> is popular beverages in Korea, and their preventive and therapeutic roles in metabolic disorders have been reported. However, the molecular mechanism has not been studied despite the known efficacy of <italic>D. morbifera</italic>. Eleven fractions (fr.1–fr.11) were divided by MPLC to find the active compound. Among them, Fr.5 was superior to others in that the inhibitory efficacy of <italic>de novo</italic> triglyceride (TG) biosynthesis. NMR analysis revealed that Fr.5 is composed 98% or more (9<italic>Z</italic>,16<italic>S</italic>)-16-hydroxy-9,17-octadecadiene-12,14-diynoic acid (HOD). Treatment of HOD diminished oleic acid (OA)-induced TG accumulation in HepG2 hepatocytes and differentiation of 3T3-L1 preadipocytes by activating LKB1/AMPK. In addition, we determined the effect of the oral administration of the extract of <italic>D. morbifera</italic> on obesity and hepatic steatosis in high-fat diet (HFD)-induced obese mice. This study proved that <italic>D. morbifera</italic> containing HOD, the active substance, can show preventive or therapeutic efficacy on obesity and hepatic steatosis through the targeting LKB1/AMPK axis.</p>