AUTHOR=Romano Alessandra , Giallongo Cesarina , La Cava Piera , Parrinello Nunziatina L. , Chiechi Antonella , Vetro Calogero , Tibullo Daniele , Di Raimondo Francesco , Liotta Lance A. , Espina Virginia , Palumbo Giuseppe A. TITLE=Proteomic Analysis Reveals Autophagy as Pro-Survival Pathway Elicited by Long-Term Exposure with 5-Azacitidine in High-Risk Myelodysplasia JOURNAL=Frontiers in Pharmacology VOLUME=8 YEAR=2017 URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2017.00204 DOI=10.3389/fphar.2017.00204 ISSN=1663-9812 ABSTRACT=
Azacytidine (5-AZA) is the standard first-choice treatment for high-risk myelodysplasia (MDS) patients. However, the clinical outcome for those patients who interrupt treatment or whose disease failed to respond is very poor. In order to identify the cellular pathways that are modified by long-term exposure to 5-AZA, we evaluated key proteins associated with the autophagy pathway by reverse-phase microarray (RPPA). Comparing bone marrow mononucleated cells (BMMCs) obtained from 20 newly-diagnosed patients and after four 5-AZA cycles we found an increased autophagy signaling. We then evaluated