AUTHOR=Bai Jingwen , Yang Yanbei , Wang Shuai , Gao Lingfei , Chen Jianqing , Ren Yongzhi , Ding Wenya , Muhammad Ishfaq , Li Yanhua 

TITLE=Syringa oblata Lindl. Aqueous Extract Is a Potential Biofilm Inhibitor in S. suis

JOURNAL=Frontiers in Pharmacology

VOLUME=8

YEAR=2017

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2017.00026

DOI=10.3389/fphar.2017.00026

ISSN=1663-9812

ABSTRACT=<p><italic>Streptococcus suis</italic> (<italic>S. suis</italic>) is a zoonotic pathogen that causes severe disease symptoms in pigs and humans. <italic>Syringa oblata Lindl.</italic> distributed in the middle latitudes of Eurasia and North America were proved as the most development potential of Chinese Medicine. In this study, biofilm formation by <italic>S. suis</italic> decreased after growth with 1/2 MIC, 1/4 MIC, or 1/8 MIC of <italic>Syringa oblata Lindl.</italic> aqueous extract and rutin. Scanning electron microscopy analysis revealed the potential effect of <italic>Syringa oblata Lindl.</italic> aqueous extract and rutin against biofilm formation by <italic>S. suis</italic>. Using iTRAQ technology, comparative proteomic analyses was performed at two conditions: 1/2 MIC of <italic>Syringa oblata Lindl.</italic> aqueous extract treated and non-treated cells. The results revealed the existence of 28 proteins of varying amounts. We found that the majority of the proteins were related to cell growth and metabolism. We also found that <italic>Syringa oblata Lindl.</italic> Aqueous extract affected the synthesis enzymes. In summary, <italic>Syringa oblata Lindl.</italic> aqueous extract might be used to inhibit the biofilm formation effectively by <italic>S. suis</italic>, and the active ingredients of the <italic>Syringa oblate Lindl.</italic> aqueous extract is rutin. The content of rutin is 9.9 ± 0.089 mg/g dry weight.</p>