AUTHOR=Wang Peng-Yun , Zhu Xiao-Ling , Lin Zhi-Bin 

TITLE=Antitumor and Immunomodulatory Effects of Polysaccharides from Broken-Spore of Ganoderma lucidum

JOURNAL=Frontiers in Pharmacology

VOLUME=3

YEAR=2012

URL=https://www.frontiersin.org/journals/pharmacology/articles/10.3389/fphar.2012.00135

DOI=10.3389/fphar.2012.00135

ISSN=1663-9812

ABSTRACT=<p>The antitumor and immunomodulatory activity of broken-spore of <italic>Ganoderma</italic><italic>lucidum</italic> polysaccharides (<italic>Gl</italic>-BSP) were investigated <italic>in vivo</italic> and <italic>in vitro</italic>. It was showed that <italic>Gl</italic>-BSP (50, 100, and 200 mg kg<sup>−1</sup>) exhibited antitumor effect against Sarcoma 180 (S180) in BALB/c mice. The <italic>Gl</italic>-BSP was not cytotoxicity in S180 cells and PG cells (human lung carcinoma cell) <italic>in vitro</italic>. However, serum from <italic>Gl</italic>-BSP-treated S180-bearing mice significantly inhibited S180 and PG cells proliferation <italic>in vitro</italic>. Moreover, <italic>Gl</italic>-BSP promoted the splenic lymphocyte proliferation induced by Con A or LPS, enhanced nature killer cell (NK cell) cytotoxic activity, augmented the percentage of neutral red phagocytosis by macrophages, and increased the percentage of the CD4<sup>+</sup> or CD8<sup>+</sup> subset in S180-bearing mice. The serum level of IFN-γ, TNF-α, and nitric oxide was increased by <italic>Gl</italic>-BSP. <italic>Gl</italic>-BSP also showed immunomodulatory activities in tumor-bearing mice. Furthermore, neutralization with anti-TNF-α and/or anti-IFN-γ significantly diminished growth inhibition induced by <italic>Gl</italic>-BSP-treated serum of S180-bearing mice in S180 or PG cells. These observations suggest that the antitumor activity of <italic>Gl</italic>-BSP may be mainly related to the activation of the immune response of the host organism by the stimulation of NK cells, T cells, and macrophages.</p>