External negative pressure improves lung aeration in near-term rabbit kittens at risk of developing respiratory distress

Introduction: As airway liquid is cleared into lung interstitial tissue after birth, the chest wall must expand to accommodate this liquid and the incoming air. We examined the effect of applying external positive and negative pressures to the chest wall on lung aeration in near-term rabbit kittens at risk of developing respiratory distress.

Methods: Rabbit kittens (30 days; term ∼31 days) were randomised into Control and Elevated Liquid (EL) groups. Lung liquid was drained in Control kittens to simulate expected volumes following vaginal delivery. EL kittens had lung liquid drained before 30 ml/kg was returned to simulate expected volumes after caesarean section. Kittens were delivered, placed in a water-filled plethysmograph and the external pressure was adjusted to −6 (negative), 0 (atmospheric), or +6 (positive) cmH₂O. Kittens were ventilated with an 8 ml/kg tidal volume and PEEP of 0 cmH₂O and lungs imaged using phase contrast x-ray imaging.

Results: Compared to external atmospheric pressures, external negative pressures expanded the chest (by 2100 ± 43 vs. 1805 ± 59 mm²; Control kittens; P = 0.028), directed tidal ventilation into lower, larger lung regions and increased functional residual capacity (FRC) levels in both Control (26.7 ± 2.0 vs. 12.6 ± 2.2 ml/kg; P < 0.001) and EL (19.6 ± 1.6 vs. 10.0 ± 2.9 ml/kg; P < 0.01) kittens. External positive pressures reduced FRC levels in Control (6.3 ± 0.8 vs. 12.6 ± 2.2 ml/kg; P < 0.05), but not in EL kittens, and directed tidal ventilation into upper lung regions.

Discussion: External negative pressures increased lung aeration and resulted in a more evenly distributed tidal ventilation immediately after birth in near-term rabbit kittens, whereas external positive pressures reduced lung aeration and compliance.

Introduction

Neonatal respiratory distress (RD) is commonly associated with preterm infants who are born with immature and surfactant-deficient lungs (1). However, RD also occurs in near-term and term infants and is the leading reason for admission into neonatal intensive care, accounting for 51.2% of infants admitted (2). However, the underlying cause of RD is very different in term and preterm infants, with the primary cause of RD in term infants being higher-than-expected volumes of liquid in the airways of those delivered by caesarean section without labour (3–6).

All liquid present in the airways at birth must be cleared into the surrounding pulmonary interstitial tissue, irrespective of whether sodium reabsorption or inspiratory efforts are responsible (7, 8). Airway liquid clearance results in pulmonary oedema that can last for hours after birth while the liquid is slowly cleared from the pulmonary interstitium via lymphatics and blood vessels (3, 8–10). As the presence of larger volumes of liquid in the airways at birth increases the degree of pulmonary oedema after birth, the adverse effects on lung function in the immediate newborn period are also increased (3).

To accommodate both the incoming air, which establishes the newly formed functional residual capacity (FRC), and the liquid that has moved from the airways into lung tissue, the chest wall must expand at birth (7). As the neonatal chest wall is highly compliant, it can easily expand to accommodate the newly formed FRC, but its capacity to expand is limited (7). Thus, if the volume of liquid in the airways at birth is greater than expected, such as in infants delivered by caesarean section without labour, the chest wall must expand further to accommodate the same FRC. However, there must be a point at which further chest wall expansion requires an intra-thoracic pressure that exacerbates the effects of pulmonary oedema and compromises lung function.

While chest wall expansion is an integral component of the respiratory transition at birth, its role has been largely overlooked as a factor contributing to respiratory function after birth. Some common delivery room practices such as tight swaddling can apply an external positive pressure of 5–10 cmH₂O to the chest wall (11). As the application of external positive pressures (∼7 cmH₂O) limits chest wall expansion and impairs gas exchange in near-term lambs, it is possible that over-swaddling can adversely affect respiratory function in newborn infants (12). Indeed, swaddling is associated with increased respiratory rates in infants, likely a compensatory mechanism resulting from a decreased FRC (11). Conversely, the application of external negative pressures can improve oxygenation in infants and can facilitate chest wall expansion, reduce mean airway pressure, increase oxygen delivery, and reduce the fraction of inspired oxygen (FiO₂) requirement in near-term newborn lambs (12–14).

The mechanisms by which external thoracic pressures impact gas exchange after birth are unknown, but they most likely affect lung aeration and function. In this study, we have used phase-contrast x-ray imaging to investigate the effects of external positive and negative pressures on lung aeration in near-term rabbit kittens at risk of developing respiratory distress after birth. We hypothesised that external positive pressures would compress the chest wall, which would subsequently compress the lungs and reduce FRC levels, thereby compounding the adverse effects of elevated airway liquid volumes. We also hypothesised that external negative pressures would expand the chest wall and increase FRC levels, thereby reducing the adverse effects of elevated airway liquid.

Materials and methods

Ethical approval

All animal procedures were approved by the SPring-8 Animal Care and Monash Medical Centre Animal Ethics Committee and conducted in accordance with the National Health and Medical Research Council (NHMRC) code of practice for the care and use of animals for scientific purposes (15). Methodological reporting is provided per the ARRIVE guidelines (16).

Experimental procedure

Pregnant Japanese White rabbit does at 30 days of gestational age (GA; term ∼31 days) were initially sedated with propofol (8 mg/kg bolus i.v., followed by 20 ml/kg/h Propofol, Nichi-Iko Pharmaceuticals Co. Ltd, Toyama, Japan). Anaesthesia was maintained following intubation with inhaled isoflurane (Isoflurane, 1.5%–4%, Viatrus Pharmaceuticals Inc., Tokyo, Japan) as previously described (3). Rabbit kittens were exteriorised via caesarean section, with the umbilical cord kept intact. The kittens were anesthetised with sodium secobarbital (250 µl i.p., Ional Sodium, Nichi-Iko Pharmaceuticals Co. Ltd, Toyama, Japan) and intubated via a tracheostomy (18G intracath; BD Australia). After intubation, airway liquid was drained from the endotracheal tube using a 1 ml syringe. Kittens were randomised into two treatment groups, Control or Elevated Liquid (EL). Control kittens received no further treatment, simulating expected airway liquid volumes following vaginal delivery with labour. Following airway liquid draining, EL kittens had 30 ml/kg Hartmann's solution added back via the endotracheal tube to simulate expected airway liquid volumes following elective caesarean section (liquid volumes drained and added are shown in Table 1). It is expected that ∼7 ml/kg remains in the distal airways following drainage, therefore, we expect that Control kittens had ∼7 ml/kg in their airways, and EL kittens had ∼37 ml/kg liquid in their airways (4, 17). The endotracheal tube was blocked to prevent loss of airway liquid before the onset of ventilation and imaging.

Table 1

Table 1. Subject characteristics in Control and Elevated Liquid (EL) kittens.

Ventilation protocol

Following delivery, kittens were placed upright and head-out in the main chamber of a U-shaped, water-filled (39 ℃) plethysmograph located in the imaging hutch as previously described (Figure 1) (3, 7). The main chamber was sealed using a rubber diaphragm that fitted snuggly around the neck of the kitten, leaving only the riser tube open to the atmosphere. Kittens were further block randomised into experimental groups allocated to receive an external pressure of either +6 cmH₂O (Control n = 8; EL n = 8), 0 cmH₂O (Control n = 7; EL n = 7), or −6 cmH₂O (Control n = 7; EL n = 7). These external pressures were achieved by adjusting the water level in the riser column of the plethysmograph; they were adjusted to be 6 cm above (+6 cmH₂O), 6 cm below (−6 cmH₂O), or at the level of the middle of each kittens' chest (0 cmH₂O) to regulate the external pressure applied (Figure 1).

Figure 1

Figure 1. Kitten positioning and plethysmograph experimental set-up. The water level in the riser tube of the plethysmograph was adjusted by +6 (positive), −6 (negative), or 0 (atmospheric) cm relative to the middle of the chest to regulate externally applied pressures. Figure created on BioRender.com.

The endotracheal tube was connected to a custom-built ventilator (18) and, once the allocated external pressures were applied, the hutch was closed and imaging commenced. Ventilation commenced with a sustained inflation (SI), with the SI pressure increasing incrementally by 2 cmH₂O/step starting from 24 cmH₂O. Then, when air first began to enter the lung, the incremental pressure increase was paused and this SI pressure was maintained until 20 ml/kg of air had entered the lungs, at which point the SI ceased. Kittens were then mechanically ventilated with a positive end-expiratory pressure (PEEP) of 0 cmH₂O, inspiratory time of 650 ms, expiratory time of 750 ms, and a tidal volume (Vt) of 8 ml/kg measured directly from the plethysmograph as previously described (3). After ∼6 min, a stepwise PEEP recruitment procedure was undertaken, with PEEP incrementally adjusted from 0 cmH₂O to 2, 4, 6, 4, 2, and 0 cmH₂O. Throughout the experimental period, airway pressure, lung gas volume (measured from the plethysmograph), external pressure, expired CO₂, and heart rate were recorded on LabChart 8 (PowerLab, ADInstruments, NSW, Australia). At the end of the experimental period, all kittens were euthanised with an overdose of sodium secobarbital (3 mg i.p. Ional Sodium, Nichi-Iko Pharmaceuticals Co. Ltd, Toyama, Japan).

Phase-contrast x-ray imaging

The study was conducted in experimental hutch 3 of beamline 20B2 in the Biomedical Imaging Centre of the SPring-8 Synchrotron Facility in Japan. The entire thoracic cavity and upper abdomen of each rabbit kitten was imaged using phase-contrast and a synchrotron x-ray radiation source tuned to an energy of 24 keV, as previously described (3, 4). The x-ray source-to-sample distance was ∼210 m, and the sample-to-detector distance was ∼2 m. A detector (Hamamatsu ORCA Quest qCMOS C15550-20UP) was coupled to a 25-μm thick gadolinium oxysulfide (Gd2O2S:Tb+) powdered phosphor and a tandem lens system that provided an effective pixel size of 17.86 μm and an active field of view of 39 (W) × 29 (H) mm². Image acquisition was triggered by the ventilator at the onset of each inflation, which triggered a train of 7 images at 20 Hz ensuring that image acquisition was synchronised with each ventilation cycle. Flat-field and dark-current images were acquired after each kitten was imaged to allow for the correction of variations in the beam intensity and detector dark current signal.

Phase-contrast x-ray image analysis

Lung gas volumes

Regional lung gas volumes were measured by phase-contrast x-ray imaging as previously described (3, 4, 19). Kittens were imaged while upright in the plethysmograph and images of the chest were divided into quadrants: upper left (UL), upper right (UR), lower left (LL), and lower right (LR). The 7th rib was used as the boundary to differentiate the upper and lower quadrants. The regional images were used to measure lung gas volumes at peak FRC, peak inflation, and tidal volume (Vt). The uniformity of lung aeration was assessed by calculating the 20-s trailing average of the coefficient of variation at 5 min after the SI, with lower values indicating more uniform aeration (20).

Tidal volume

The tidal volume heatmaps were calculated using a regional adaption of the method previously presented (19). A 256-pixel wide scanning window was used to calculate the regional projected thickness of air throughout the lungs at both FRC and peak inspiration. The pixel-wise sum of each regional projected thickness image was normalised to the total lung air volume calculated using a region that covered the whole chest. Regional tidal volume was then obtained by subtracting the resulting FRC image from the resulting peak inspiration image. This process was repeated for all kittens and coloured on a scale bounded by the minimum and maximum regional tidal volume.

Chest wall measurements

Image J (National Institute of Health, Maryland, USA) (21) was used to measure the thoracic area before aeration and at both maximum FRC and inspiration using a manually drawn region of interest (ROI) bounded by the 1st and 9th rib and the “measure” function. Between the left and right 9th rib, the ROI was drawn to the diaphragm peak in the FRC images, with the vertebra that matched the diaphragm height used in the unaerated images. The thoracic area was corrected for the length between the 1st and 5th vertebrae measured for each kitten on ImageJ to adjust for body size. The radius of diaphragm curvature at FRC was calculated by fitting a circular ROI to the outline of the diaphragm and corrected for the length between the 1st and 5th vertebrae. A single observer (CD) was blinded while analysing phase contrast x-ray images on ImageJ.

Physiological data analysis

Physiological parameters measured on LabChart v8 (ADInstruments, Sydney, Australia) were analysed (20-s epochs every 1 min for 6 min; starting immediately following the sustained inflation). Dynamic respiratory system compliance (C_RS) was calculated from ventilation parameters [C_RS = Vt (PIP − PEEP)]. As expired CO₂ (relative measure) was measured using a CO₂ analyser located in the ventilator, not at the mouth opening, expired CO₂ data was time-shifted to synchronise the expired CO₂ recording with other physiological data as previously described (9).

Statistical analysis

A power analysis was conducted to determine the required sample size (∼7 kittens per group) for the primary outcome of thoracic expansion based on a previous study (3). The power was set at 0.8, and the type one error rate to 0.5. This study aimed to investigate the impact of positive (+6 cmH₂O) and negative (−6 cmH₂O) external pressures on lung aeration in Control and EL kittens compared to controls at atmospheric pressure (0 cmH₂O). All data were assessed for normality on IBM SPSS Statistics (Version 29) and logarithmically transformed if required. Continuous data were analysed using a repeated measures general linear model followed by a Sidak-corrected estimated means pairwise comparisons in IBM SPSS Statistics. Comparisons were made to analyse the effect of external pressure in Control and EL kittens, as well as the impact of airway liquid volumes on lung aeration. The thoracic area before lung aeration and at maximum FRC were analysed using a one-way ANOVA. Regional tidal ventilation data were analysed with a repeated-measures two-way ANOVA. Subject characteristics (Table 1) and plethysmograph pressure were presented as mean ± SD and all remaining data were presented as mean ± SEM. P ≤ 0.05 were considered statistically significant.

Results

Animal inclusion and subject characteristics

Nine rabbit does and 57 kittens were initially used for this study, but one doe and her litter (9 kittens) were excluded due to premature delivery. Four kittens from the remaining 8 does were excluded due to surgical/imaging complications. All remaining kittens (44 kittens from 8 does) were included in the analysis, and all had similar body weights, volume of lung liquid drained, and volume of lung liquid added to the airways of EL kittens, between groups (Table 1).

Plethysmograph pressure

During the application of external atmospheric pressure, the mean (SD) pressure in the plethysmograph was 0.5 ± 1.0 cmH₂O (Control) and 0.7 ± 0.7 cmH₂O (EL) at the end of the experimental period. During application of external positive pressures, the pressure in the plethysmograph was 5.5 ± 0.6 cmH₂O in Control kittens and 5.7 ± 0.8 cmH₂O in EL kittens. Before lung aeration, the external negative pressures applied were −5.5 ± 0.1 cmH₂O below atmospheric pressure in Control kittens and −5.7 ± 0.2 cmH₂O in EL kittens. However, 5 min after the sustained inflation (SI), the pressure in the plethysmograph had increased to −2.7 ± 0.8 cmH₂O in Control kittens and −3.7 ± 0.3 cmH₂O in EL kittens. Both external positive and negative pressures were significantly different from the atmospheric pressure group (P < 0.0001).

Air distribution during tidal ventilation

In Control kittens, the distribution of tidal ventilation within the lung was significantly related to the external pressure applied. As the level of external pressure decreased, the distribution of ventilation was redirected towards the lower lung regions (LL, R² = 0.30; P = 0.008; and LR, R² = 0.23; P = 0.02; Figures 2, 3) and away from the upper lung regions (UL, R² = 0.32; P = 0.006; and UR, R² = 0.15; P = 0.072). While this relationship was not significant in EL kittens, the trend appeared to be similar for the left lung, but not for the right lung (Figures 2, 3).

Figure 2

Figure 2. Representative colour maps showing the regional distribution of air during tidal ventilation. Regional tidal volume was calculated by subtracting functional residual capacity from peak gas volume at the end of the experimental period in Control and Elevated Liquid (EL) kittens exposed to external positive, atmospheric, and negative pressures.

Figure 3

Figure 3. Regional distribution of tidal volume in the upper left, upper right, lower left, and lower right quadrants of the lung during tidal ventilation (% of total). Bars that do not share a common letter are significantly different from each other (P ≤ 0.05); A, B, C, D & E, differences between lung regions within treatment groups (repeated measures one-way ANOVA with Tukey's multiple comparisons); x, y, differences between treatment groups within lung regions (one-way ANOVA with Tukey's multiple comparisons). Asterisks indicate linear regression significance from zero evaluating the relationship between external pressure and distribution of tidal ventilation. Mean ± SEM.

External pressures in Control kittens

In Control kittens, the proportion of tidal volume entering the UL and UR lung regions were significantly greater in kittens exposed to positive compared to negative external pressures (UL; 24.5 ± 2.3% vs. 15.2 ± 1.4%; P = 0.047; UR; 28.2 ± 5.0% vs. 17.9 ± 0.7%; P = 0.017). In contrast, the percentage of tidal volume entering the LL lung region was significantly greater in kittens exposed to negative compared to positive external pressures (33.5 ± 0.9% vs. 22.9 ± 2.6%; P = 0.013). Similarly, the percentage of tidal volume entering the LR lung region tended to be greater in kittens exposed to negative compared to positive external pressures, although this difference was not quite significant (33.4 ± 1.8 vs. 24.5 ± 2.9%; P = 0.060; Figure 3). The percentage of tidal volume entering the lung quadrants in kittens exposed to atmospheric pressure were not significantly different compared to kittens exposed to negative and positive external pressures (Figures 2, 3).

External pressures in EL kittens

In EL kittens, the proportion of tidal volume entering the lung quadrants were not significantly different between kittens exposed to positive, atmospheric or negative external pressures (Figures 2, 3).

When EL kittens were exposed to external atmospheric pressures, a significantly greater proportion of tidal volume entered the LL compared to the UL or UR regions of the lungs (LL, 31.8 ± 3.3%; UL, 18.9 ± 1.8%; UR, 22.2 ± 1.3%; P < 0.05; Figure 3). Similarly, external negative pressure increased the proportion of tidal ventilation in the lower compared to the upper lung regions (LL, 32.8 ± 1.6; LR, 31.3 ± 1.3; UL, 16.9 ± 0.8; UR, 19.0 ± 0.6%; P < 0.01; Figure 3).

Chest wall mechanics

Effect of elevated airway liquid volumes

Thoracic area before lung aeration was significantly greater in EL kittens exposed to external negative pressures compared to Control kittens exposed to atmospheric (2,044 ± 60 vs. 1,703 ± 64 mm²; P = 0.013) and positive external pressures (2,044 ± 60 vs. 1,676 ± 85 mm²; P = 0.004; Figure 4A), but was not significantly different from thoracic area in Control kittens exposed to external negative pressures (2,044 ± 60 vs. 1,843 ± 61 mm²). Similarly, after lung aeration, the thoracic area (measured at FRC) was greater in EL kittens exposed to external negative pressures than Control kittens exposed to atmospheric (2,227 ± 67 vs. 1,805 ± 59 mm²; P = 0.001) and positive external pressures (2,227 ± 67 vs. 1,680 ± 67 mm²; P < 0.0001). There was no significant difference between thoracic area (measured at FRC) in Control and EL kittens exposed to external negative pressures. The thoracic area was also greater in EL kittens exposed to external atmospheric pressures than Control kittens exposed to external positive pressures, measured after lung aeration at FRC (1,987 ± 79 vs. 1,680 ± 67 mm²; P = 0.015; Figure 4B).

Figure 4

Figure 4. Thoracic area standardised by the length (mm²) between the 1–5th vertebrae. Thoracic area of Control and Elevated Liquid (EL) kittens are shown (A) before lung aeration and (B) at functional residual capacity (FRC); data were analysed with a one-way ANOVA followed by Tukey's multiple comparisons. Points that do not share a common letter are significantly different from each other (P ≤ 0.05). (B) presents a linear regression assessing the relationship between external pressure and thoracic area († indicates significance within the Control group, †††P < 0.001; and # indicates significant differences within the EL group, #P < 0.05). Thoracic area data before lung aeration and at FRC are shown in (C) Control and (D) EL kittens and were analysed with a repeated measures two-way ANOVA followed by Šídák's multiple comparisons. * indicates significance between unaerated and FRC thoracic areas in each treatment group. *P < 0.05, ****P < 0.0001. Mean ± SEM.

Effect of external pressures in Control kittens

Before lung aeration, the thoracic area was similar in Control kittens exposed to external positive, atmospheric, and negative pressure, although tended to increase with decreasing pressure (Figure 4A). However, after lung aeration, thoracic area (measured at FRC) was significantly greater in Control kittens exposed to external negative pressures than kittens exposed to positive (2,100 ± 43 vs. 1,680 ± 67 mm²; P = 0.0004) and atmospheric (2,100 ± 43 vs. 1,805 ± 59 mm²; P = 0.028; Figure 4B) external pressures.

In Control kittens, aeration of the lung increased the thoracic area (measured at FRC) in kittens exposed to both atmospheric (from 1,703 ± 64 to 1,805 ± 59 mm²; P = 0.013) and negative (from 1,843 ± 61 to 2,100 ± 43 mm²; P < 0.0001; Figure 4C) external pressures. However, thoracic area was similar before and after lung aeration in Control kittens exposed to external positive pressures. External pressures did not affect the curvature of the diaphragm or chest wall movement during tidal ventilation in Control kittens (Table 2).

Table 2

Table 2. Measurements of ventilation, lung, and thoracic parameters.

Effect of external pressures in EL kittens

Before lung aeration, the thoracic area of EL kittens was similar in kittens exposed to external positive, atmospheric, and negative pressures (Figure 4A), but tended to increase with decreasing pressure. However, after lung aeration, the thoracic area (measured at FRC) was significantly greater in kittens exposed to external negative pressures than external positive pressures (2,227 ± 67 vs. 1,915 ± 57 mm²; P = 0.013; Figure 4B).

Lung aeration significantly increased the thoracic area (measured at FRC) in EL kittens exposed to external negative pressures (2,044 ± 60 to 2,227 ± 67 mm²; P < 0.0001), but not in kittens exposed to external positive or atmospheric pressures (Figure 4D). External negative pressures increased the flattening of the diaphragm compared to external atmospheric (2.28 ± 0.22 vs. 1.66 ± 0.11 mm²; P = 0.011) and positive pressures (2.28 ± 0.22 vs. 1.58 ± 0.10 mm²; P = 0.002; Figure 4D), indicated by a larger radius of curvature (Table 2). External pressures did not affect chest wall movement during tidal ventilation in EL kittens (Table 2).

Respiratory system compliance (C_RS) and peak inflation pressures (PIP)

Effect of external pressures in Control kittens

In Control kittens, external positive pressures reduced C_RS compared to external atmospheric pressures following the SI (9.6 ± 0.5 vs. 32.1 ± 5.3 µl/cmH₂O at 5 min; P < 0.001; Figure 5A). In contrast, external negative pressures increased C_RS compared to atmospheric pressures at 5 min after the SI (46.5 ± 5.3 vs. 32.1 ± 5.3 µl/cmH₂O; P = 0.02) and increased C_RS compared to positive pressures (46.5 ± 5.3 vs. 9.6 ± 0.5 µl/cmH₂O at 5 min; P < 0.001; Figure 5A).

Figure 5

Figure 5. Respiratory system compliance (µl/cmH₂O; C_RS) after completion of the sustained inflation (SI). C_RS is shown in (A) Control kittens exposed to external positive (n = 8), atmospheric (n = 7), and negative (n = 7) pressures, as well as in (B) Elevated Liquid (EL) kittens exposed to external positive (n = 8), atmospheric (n = 7), and negative (n = 7) pressures. C_RS is compared in Control and EL kittens exposed to (C) atmospheric, (D) positive, and (E) negative external pressures. Data were logarithmically transformed, analysed with a general linear model with Sidak-corrected comparisons, and presented as mean ± SEM. A # indicates statistical significance between kittens in atmospheric and positive pressure (###P < 0.001), * indicates significant differences between kittens in atmospheric and negative pressure (*P ≤ 0.05, **P < 0.01), and ^ indicates significant differences between kittens in positive and negative pressures (^^^P < 0.001). Significance between Control and EL liquid kittens are indicated by daggers (†) in (C) and (E) (^†P ≤ 0.05, ^†††P < 0.001).

Furthermore, external positive pressures increased the PIP required to maintain Vt compared to both atmospheric (31.9 ± 1.0 vs. 12.4 ± 2.0 cmH₂O; P < 0.0001) and negative external pressures (31.9 ± 1.0 vs. 7.5 ± 1.0 cmH₂O; P < 0.0001; Table 2).

Effect of external pressures in EL kittens

In EL kittens, external negative pressures increased C_RS compared to both atmospheric (24.2 ± 2.3 vs. 10.6 ± 0.5 µl/cmH₂O at 5 min; P < 0.001) and positive (24.2 ± 2.3 vs. 10.0 ± 0.6 µl/cmH₂O at 5 min; P < 0.001; Figure 5B) external pressures. C_RS was similar in EL kittens exposed to positive and atmospheric external pressures.

PIP was significantly lower in EL kittens exposed to external negative pressures than positive (14.6 ± 1.7 vs. 32.9 ± 0.7; P < 0.0001) or atmospheric (14.6 ± 1.7 vs. 28.8 ± 1.2; P < 0.0001; Table 2) external pressures.

Effect of elevated airway liquid volumes

C_RS was increased after the SI in Control compared to EL kittens exposed to atmospheric (32.1 ± 5.3 vs. 10.6 ± 0.5 µl/cmH₂O at 5 min; P < 0.001; Figure 5C) and negative (46.5 ± 5.3 vs. 24.2 ± 2.3 µl/cmH₂O at 5 min; P < 0.001; Figure 5E) external pressures. However, C_RS was similar in Control and EL kittens exposed to external positive pressures (Figure 5D).

The required PIP levels were significantly higher in EL than in Control kittens exposed to atmospheric (28.8 ± 1.2 vs. 12.4 ± 2.0; P < 0.0001) and negative (14.6 ± 1.7 vs. 7.5 ± 1.0; P = 0.007) external pressures but were not different in kittens exposed to external positive pressures (Table 2).

Lung aeration

Effect of external pressures in Control kittens

In Control kittens, external positive pressures reduced FRC levels compared to external atmospheric pressures after the SI (6.2 ± 0.9 vs. 13.1 ± 2.5 ml/kg at 240 s; P = 0.04; Figure 6A) but did not affect the presence of lung bulging or the uniformity of lung aeration (Table 2). Conversely, external negative pressures increased FRC levels compared to kittens exposed to external atmospheric pressures (26.7 ± 2.0 vs. 12.6 ± 2.2 ml/kg at 330 s; P < 0.001; Figure 6A). Despite increasing FRC levels, external negative pressures did not increase the presence of lung bulging at either FRC or peak inflation (Table 2). Neither external positive nor negative pressures influenced the uniformity of lung aeration compared to external atmospheric pressures at FRC in Control kittens (Table 2).

Figure 6

Figure 6. Functional residual capacity (ml/kg; FRC) after completion of the sustained inflation (SI). FRC is shown in (A) Control kittens exposed to external positive (n = 8), atmospheric (n = 6), and negative (n = 7) pressures, as well as in (B) Elevated Liquid (EL) kittens exposed to external positive (n = 8), atmospheric (n = 7), and negative (n = 7) pressures. FRC is compared in Control and EL kittens exposed to (C) atmospheric, (D) positive, and (E) negative external pressures. Data were analysed with a general linear model with Sidak-corrected comparisons and presented as mean ± SEM. A # indicates statistical significance between kittens in atmospheric and positive pressure (#P ≤ 0.05), * indicates significant differences between kittens in atmospheric and negative pressure (**P < 0.01, ***P < 0.001), and ^ indicates significant differences between kittens in positive and negative pressures (^^^P < 0.001). Significance between Control and EL liquid kittens are indicated by daggers (†) in (C) and (E) (^†P < 0.05).

Effect of external pressures in EL kittens

In EL kittens, external negative pressures significantly increased FRC levels compared to EL kittens exposed to atmospheric pressure (19.6 ± 1.6 vs. 10.0 ± 2.9 ml/kg at 330 s; P = 0.003; Figure 6B). Increased FRC levels were not associated with an increased presence of lung bulging at either FRC or peak inflations and the uniformity of aeration was not influenced by external negative pressure (Table 2). In kittens exposed to external positive pressures, FRC levels, the uniformity of lung aeration at FRC, and the presence of lung bulging were not different compared to kittens exposed to external atmospheric pressures (Figure 6B; Table 2).

Effect of elevated airway liquid volumes

FRC levels were higher in Control than EL kittens exposed to external atmospheric pressures (from 30 to 90 s; 15.5 ± 2.9 vs. 10.0 ± 2.2 ml/kg at 90 s; P = 0.035; Figure 6C) and negative pressure (26.7 ± 2.0 vs. 19.6 ± 1.6 ml/kg at 330 s; P = 0.012; Figure 6E). When exposed to an external positive pressure, FRC levels were similar in Control and EL kittens (Figure 6D). The uniformity of lung aeration at FRC and presence of lung bulging was not different in Control and EL kittens exposed to either external atmospheric, positive, or negative pressures (Table 2).

Expired CO₂ levels

Effect of external pressures in Control kittens

In Control kittens, an external positive pressure reduced expired CO₂ levels compared to kittens in both external atmospheric (AUC = 0.23 ± 0.07 vs. 1.61 ± 0.41; P = 0.0004) and negative pressures (AUC = 0.23 ± 0.07 vs. 1.13 ± 0.18; P = 0.029). Expired CO₂ levels were not different between Control kittens exposed to external atmospheric and negative pressures (Figure 7A).

Figure 7

Figure 7. Expired CO₂ (relative measure) after completion of the sustained inflation (SI). Expired CO₂ is shown in (A) Control kittens exposed to external positive (n = 8), atmospheric (n = 7), and negative (n = 7) pressures, as well as in (B) Elevated Liquid (EL) kittens exposed to external positive (n = 8), atmospheric (n = 7), and negative (n = 7) pressures. Expired CO₂ is compared in Control and EL kittens exposed to (C) atmospheric, (D) positive, and (E) negative external pressures. Area under the curve (AUC) data were analysed with a one-way ANOVA followed by Tukey's multiple comparisons (A & B) or an unpaired t-test (C, D, & E). Mean ± SEM. Significance is shown by AUC graphs (A & B) or daggers (†) in (C) (*P < 0.05, **P < 0.01, ***P < 0.001, ^††P < 0.01).

Effect of external pressures in EL kittens

Expired CO₂ levels were significantly higher in EL kittens exposed to external negative pressures than external atmospheric (AUC = 0.93 ± 0.21 vs. 0.37 ± 0.14; P = 0.0004) and positive pressures (AUC = 0.93 ± 0.21 vs. 0.22 ± 0.12; P = 0.0004). There were no differences in expired CO₂ levels between EL kittens exposed to external positive and atmospheric pressures (Figure 7B).

Effect of elevated airway liquid volumes

Expired CO₂ levels were significantly higher in Control than EL kittens exposed to external atmospheric pressures (area under the curve = 1.63 ± 0.41 vs. 0.37 ± 0.14; P = 0.005; Figure 7C). Expired CO₂ levels were not different in Control and EL kittens exposed to external positive (Figure 7D) and negative pressures (Figure 7E).

Positive end-expiratory pressures vs. external negative pressures; effect on FRC

Control kittens

In Control kittens, the FRC levels achieved when applying an external negative pressure (26 ± 2 ml/kg) with 0 PEEP were similar to that achieved when applying external atmospheric pressures with a PEEP of 4 (20 ± 2 ml/kg) and 6 cmH₂O (25 ± 2 ml/kg) (Figures 8, 9A). While a PEEP of 4 cmH₂O tended to result in lower FRCs than external negative pressures, this difference was not significant, whereas the FRC values achieved with a PEEP of 2 cmH₂O were significantly lower than those achieved with negative pressure (26 ± 2 vs. 16 ± 2 ml/kg; P = 0.009; Figures 8, 9A).

Figure 8

Figure 8. Representative colour maps of regional functional residual capacity (FRC; ml/kg) in Control and Elevated Liquid (EL) kittens. FRC is shown in kittens exposed to external negative pressures (−6 cmH₂O) with 0 cmH₂O positive end-expiratory pressure (PEEP), and external atmospheric pressures (0 cmH₂O) with 2, 4, and 6 cmH₂O PEEP.

Figure 9

Figure 9. Functional Residual Capacity (FRC; ml/kg) levels in (A) Control and (B) Elevated Liquid (EL) kittens. FRC is shown in kittens exposed to external negative pressures (−6 cmH₂O) with 0 cmH₂O positive end-expiratory pressure (PEEP), and external atmospheric pressures (0 cmH₂O) with 2, 4, and 6 cmH₂O PEEP. 2, 4, and 6 cmH₂O PEEP groups were compared to the external negative pressure group with a one-way ANOVA followed by Dunnett's multiple comparisons and presented as mean ± SEM. **P < 0.01.

EL kittens

In EL kittens, FRC levels were similar between kittens exposed to external negative pressures and kittens exposed to external atmospheric pressures with a PEEP of 2, 4, or 6 cmH₂O (Figures 8, 9B). However, PEEP levels of 2 and 4 cmH₂O in external atmospheric pressure tended to achieve lower FRC levels than external negative pressure.

Discussion

The findings of this study add to the growing body of evidence indicating that applying external pressures to the chest wall can have a major impact on respiratory function in newborns. This study demonstrated that the application of external negative pressures expanded the chest wall, increased C_RS and FRC levels, and redirected tidal ventilation to the larger lower lobes of Control and EL kittens. Thus, external negative pressures can improve lung aeration and the distribution of ventilation in the immediate newborn period in near-term rabbit kittens, particularly in kittens with elevated airway liquid. In contrast, external positive pressures significantly reduced C_RS and lung aeration (FRC levels) and redirected tidal ventilation into the upper lobes of Control kittens. As swaddling can apply external pressures of ∼5–10 cmH₂O (11), which are similar to the pressures applied in this study, over-swaddling infants in the immediate newborn period should be avoided.

Air distribution during tidal ventilation

The application of external pressures had a major impact on the regional distribution of air during tidal ventilation. External negative pressures directed the distribution of tidal ventilation into the larger lower lung quadrants in both Control and EL kittens, which may partly explain the increase in C_RS associated with negative external pressures. Combined with the lower PIPs required to achieve a set tidal volume, external negative pressures would be expected to reduce the risk of ventilation-induced lung injury. Furthermore, as the lower lobes are larger and have a greater surface area (22), directing tidal ventilation into the lower lobes likely explains the increased oxygenation that occurs with external negative pressures (12, 13, 23). In contrast, in Control kittens, external positive pressures redirected the distribution of tidal ventilation from the lower lung quadrants into the upper lung quadrants during mechanical ventilation, although this was less evident in EL kittens. As the upper lobes of the lung are considerably smaller than the lower lobes (22), this may partly explain the decrease in C_RS and FRC levels associated with external positive pressures. As we were using a set tidal volume (8 ml/kg), forcing air to preferentially enter the upper lobes increases the risk of over-expansion and injury in those lobes.

It is also possible that the effect of external pressures on the distribution of tidal ventilation within the lung was in part due to pressures applied to the abdomen. For instance, in addition to the effect on the chest wall, a positive external pressure would also be expected to pressurise the abdomen and displace the diaphragm higher into the chest, which may cause a differential decrease in FRC and compliance across the lung, with the decrease being greatest in the lower lobes and least in the upper lobes.

Chest wall mechanics

As the neonatal chest wall is highly compliant (24), it can easily expand to accommodate both the volume of air that fills the airways and the liquid that has been cleared from the airways into the surrounding lung tissue during lung aeration (7, 8, 12). However, when infants are delivered with greater than expected volumes of liquid in their airways, which likely occurs in infants delivered by caesarean section without labour, the chest wall must expand to a greater degree to accommodate that liquid and the same volume of air (FRC). While the chest wall is highly compliant, its capacity to expand is limited and, at some point, the intra-thoracic pressure required to expand the chest further must increase. Increased intra-thoracic pressures will reduce the volume of air that is retained in the lung at FRC, as well as the volume of air that can enter and leave the lung during tidal ventilation. Thus, elevated airway liquid reduces FRC and C_RS and is now considered to be the primary factor causing respiratory distress in term/near-term infants (3, 6).

We hypothesised that external negative pressures would expand the chest wall and increase FRC levels and C_RS, particularly in kittens with elevated airway liquid, whereas positive pressures would compress and restrict chest wall movement. Interestingly, although we were unable to detect a significant effect of external pressure on chest wall dimensions, we found a significant negative linear relationship between external pressure and chest wall dimensions in both Control and EL kittens. While the application of external positive pressures did not affect chest wall movement during tidal ventilation, this is likely because the kittens were mechanically ventilated. They needed a higher peak inflation pressure to achieve the same tidal volume, thereby resulting in a similar chest wall movement. Thus, if we had used the same peak inflation pressures between groups, it is likely that we would have found that positive pressure reduces chest wall movement, as observed in previous studies (12).

The effect of elevated airway liquid on chest wall expansion before lung aeration is evident in Figure 4, where the relationship between chest wall expansion and external pressure is displaced towards a higher dimension. As a result, at the beginning of lung aeration, the chest wall may have been closer to its physiological expansion limit in EL kittens. Nevertheless, negative pressures increased chest wall expansion further which likely explains the much lower C_RS in EL compared to Control kittens (Figure 5). The increase in chest wall expansion in kittens exposed to external negative pressures likely includes flattening of the diaphragm, a feature that has previously been observed in EL kittens (3). Diaphragm flattening was significant in EL kittens, but not in Control kittens, indicating that the need to accommodate larger volumes of liquid within lung tissue is a major contributor to chest wall expansion. However, it is also possible that applying an external negative pressure to the abdomen reduced intra-abdominal pressures, allowing the apex of the diaphragm to be displaced into the abdomen, causing it to appear flattened.

It is also important to note that while external negative pressures were set to −6 cmH₂O before lung aeration, the external pressures had increased to −2.7 ± 0.8 cmH₂O after lung aeration. The increase in the external pressure applied was a result of chest wall expansion during lung aeration, which displaced liquid out of the main chamber of the plethysmograph and into the riser column, thus reducing the negative pressure. This change in external pressure associated with lung aeration was greatest in the external negative pressure group as the degree of chest wall expansion was greatest in these kittens. As we could not adjust the external pressure without disrupting the phase contrast X-imaging, kittens in the external negative pressure group were exposed to a lesser negative pressure than intended following lung aeration. Nevertheless, these reduced negative pressures had a marked effect on FRC levels, C_RS, CO₂ clearance, and the distribution of tidal ventilation.

Respiratory function

Respiratory system compliance (C_RS) is composed of both chest wall and lung compliance, but in the neonate, it is predominantly a measure of lung compliance (C_L) as chest wall compliance (C_CW) is so high $\left(\frac{1}{C_{\mathrm{RS}}}=\frac{1}{C_L}+\frac{1}{C_{\mathrm{CW}}}\right)$ (25). Thus, the increase and reduction in C_RS in Control kittens exposed to external negative and positive pressures, respectively, are likely due to changes in lung compliance. These changes could result from the effect that external negative and positive pressures had on FRC levels, placing the lung in more or less compliant regions of the pressure/volume curve (26).

As external negative pressures increased FRC levels in EL kittens, which would be expected to increase alveolar recruitment and gas exchange surface area, it is not surprising that expired CO₂ levels were also increased in EL kittens. While expired CO₂ levels were similar in Control and EL kittens exposed to external negative pressures, indicating greatly improved CO₂ exchange in EL kittens, FRC levels were higher in Control kittens. These findings indicate that FRC levels in both groups had increased above the level at which CO₂ exchange is surface area-limited, and are consistent with previous studies demonstrating the benefits of external negative pressures on oxygenation (12, 13, 23).

In contrast, external positive pressures decreased the expired CO₂ levels in Control kittens in addition to reducing lung compliance. As CO₂ is ∼27 times more soluble than oxygen, a reduction in the exchange of CO₂ across the lungs indicates that oxygen exchange must also have been reduced as previously observed in lambs (12). Impaired oxygen exchange is consistent with the finding that external positive pressures reduce FRC levels in Control kittens, which is indicative of a reduced alveolar surface area available for gas exchange.

Interestingly, in EL kittens, external positive pressures did not reduce C_RS, FRC, and expired CO₂ levels compared to external atmospheric pressures, demonstrating that external positive pressures do not further impair respiratory function in kittens whose respiratory function is already compromised. Indeed, in kittens exposed to external positive pressure, C_RS, FRC, and expired CO₂ levels were similar in EL and Control kittens, demonstrating that these factors are negatively influenced by positive pressures, but that the impacts of EL and external positive pressures are not additive.

External negative pressures vs. positive end-expiratory pressures

It is well established that PEEP plays an important role in both the creation and maintenance of FRC levels in mechanically ventilated preterm newborns at birth (27, 28). Without PEEP, the airways are prone to collapse and the distal airways reflood at end-expiration, which limits gas exchange to the period when the lungs are inflated as there is little to no air in the airway at FRC for gas exchange to occur (29). As external negative pressures are commonly thought to replicate PEEP (30, 31), we compared the effect of external negative pressures to different PEEP levels on FRC following lung aeration. We found that external negative pressures of approximately −3 cmH₂O were able to replicate the effect of a PEEP of 4–6 cmH₂O on FRC levels in both Control and EL kittens.

Summary

We have shown that external pressures have a large impact on lung aeration, respiratory system compliance, and the distribution of ventilation within the lung in newborn near-term rabbit kittens. External positive pressures, which are not too dissimilar to the pressures applied to the chest by tight swaddling [5–10 cmH₂O (11)] reduced C_RS, FRC levels, expired CO₂ levels, and redirected tidal ventilation into the upper lung lobes. In contrast, the application of external negative pressures (approximately −3 cmH₂O) expanded the chest wall, and increased C_RS, FRC levels, expired CO₂ levels, and redirected tidal ventilation to the larger lower lung lobes. Additionally, we have shown that external negative pressures are as effective as a PEEP of 4–6 cmH₂O in maintaining FRC levels. Thus, the results from this study indicate that external negative pressures may be an alternative approach for assisting near-term and term infants with respiratory distress.

Limitations

We aimed to apply external negative pressures of −6 cmH₂O in this study. However, aeration of the lungs and subsequent expansion of the chest wall displaced the water in the plethysmograph, thereby decreasing the magnitude of the external negative pressure applied. The chest wall expansion was greatest in the kittens exposed to external negative pressures, resulting in applied external negative pressures of approximately −3 cmH₂O instead of the targeted −6 cmH₂O. In contrast, the chest wall expanded less in kittens exposed to external atmospheric and positive pressures, so the applied pressures were closer to the intended levels of 0 and 6 cmH₂O, respectively.

Data availability statement

The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.

Ethics statement

The animal study was approved by SPring-8 Animal Care and Monash Medical Centre Animal Ethics Committee. The study was conducted in accordance with the local legislation and institutional requirements.

Author contributions

CD: Conceptualization, Formal Analysis, Investigation, Methodology, Writing – original draft, Writing – review & editing. KC: Conceptualization, Funding acquisition, Investigation, Methodology, Resources, Supervision, Writing – review & editing. DJ: Formal Analysis, Investigation, Methodology, Resources, Writing – review & editing. MW: Investigation, Methodology, Writing – review & editing. ID: Investigation, Writing – review & editing. PR: Investigation, Writing – review & editing. AtP: Conceptualization, Investigation, Methodology, Writing – review & editing. MK: Formal Analysis, Investigation, Methodology, Resources, Writing – review & editing. SH: Conceptualization, Formal Analysis, Funding acquisition, Investigation, Methodology, Supervision, Writing – original draft, Writing – review & editing.

Funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by a National Health and Medical Research Council (NHMRC) Ideas Grant (2012443 & 2021055) and the Victorian Government's Operational Infrastructure Support Program. CD was supported by the Australian Government Research Training Program Scholarship. KC and ID were supported by the Australian Synchrotron International Synchrotron Access Program (AS/IA223/19741). SH was supported by a Senior NHMRC Principal Research Fellowship (APP1154914).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision.

Generative AI statement

The authors declare that no Generative AI was used in the creation of this manuscript.

Publisher's note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fped.2024.1526603/full#supplementary-material

Supplementary Video S1 | Representative videos of lung aeration from the first breath after the sustained inflation ceased.

Abbreviations

AUC, area under the curve; C_CW, chest wall compliance; C_L, lung compliance; CO₂, carbon dioxide; C_RS, respiratory system compliance; EL, elevated liquid; FiO₂, fraction of inspired oxygen; FRC, functional residual capacity; GA, gestational age; LL, lower left; LR lower right; O₂, oxygen; PIP, peak inflation pressure; PEEP, positive end-expiratory pressure; RD, respiratory distress; SI, sustained inflation; UL, upper left; UR, upper right; Vt, tidal volume.

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