AUTHOR=Aziz Cylmara , Morales Armando , Pinto Walter , Fanchini Vanessa , Dell Aquila Luis , Sangaleti Carine , Elias Rosilene , Dalboni Maria 

TITLE=Evaluation of IL-6, FoxP3 Treg lymphocytes, intestinal barrier biomarkers and the use of synbiotics in obese adolescents: a pilot study

JOURNAL=Frontiers in Pediatrics

VOLUME=11

YEAR=2023

URL=https://www.frontiersin.org/journals/pediatrics/articles/10.3389/fped.2023.1215793

DOI=10.3389/fped.2023.1215793

ISSN=2296-2360

ABSTRACT=<sec><title>Aim</title><p>This prospective pilot study evaluated inflammatory and intestinal barrier biomarkers and the effects of a synbiotic in obese adolescents.</p></sec><sec><title>Methods</title><p>Eighteen obese and 20 eutrophic adolescents were evaluated for body composition using bioimpedance analysis (BIA), body mass index (BMI), IL-6 and lipopolysaccharide (LPS) serum levels, CD4 and FoxP3 Treg lymphocytes and monocytes. Synbiotic supplementation for 60 days was also evaluated for these parameters only in obese adolescents.</p></sec><sec><title>Results</title><p>We observed an increase in CD4 lymphocyte (18.0 ± 12.4 vs. 8.9 ± 7.5; <italic>p</italic> &lt; 0.01), IL-6 (0.30 ± 0.06 vs. 0.20 ± 0.06; <italic>p</italic> = 0.02) and LPS (0.18 ± 0.15 vs. 0.08 ± 0.05; <italic>p</italic> &lt; 0.01) levels in obese compared to eutrophic adolescents. After synbiotic supplementation, FoxP3 Treg lymphocytes increased (14.0 ± 6.7 vs. 9.9 ± 5.4; <italic>p</italic> = 0.02) in obese adolescents.</p></sec><sec><title>Conclusions</title><p>Obese adolescents presented a state of microinflammation and intestinal barrier breakdown, and synbiotic supplementation increased the expression of FoxP3 Treg lymphocytes, an anti-inflammatory regulator. Whether the increase in FoxP3 Treg lymphocytes may have an impact on inflammation and outcomes in obese adolescents deserves further evaluation.</p></sec>