AUTHOR=He Guan-nan , Wang Xue-yan , Kang Min , Chen Xi-min , Xi Na , Zhao Jing , Chen Xi 

TITLE=Prenatal Diagnosis of Holt–Oram Syndrome With a Novel Mutation of TBX5 Gene: A Case Report

JOURNAL=Frontiers in Pediatrics

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/pediatrics/articles/10.3389/fped.2021.737633

DOI=10.3389/fped.2021.737633

ISSN=2296-2360

ABSTRACT=<p><bold>Background:</bold> Holt–Oram syndrome (HOS) is an autosomal dominant disorder caused by mutations of <italic>TBX5</italic> gene.</p><p><bold>Case presentation:</bold> We report a fetus with HOS diagnosed sonographically at 23 weeks of gestation. The fetal parents are non-consanguineous. The fetus exhibited short radius and ulna, inability to supinate the hands, absence of the right thumb, and heart ventricular septal defect (VSD), while the fetal father exhibited VSD and short radius and ulna only. Fetal brother had cubitus valgus and thumb adduction, except for VSD, short radius and ulna. The pregnancy was terminated. Whole-exome sequencing (WES) revealed a novel mutation in the <italic>TBX5</italic> (c.510+1G&gt;A) in the fetus inherited from the father. The variant (c.510+1G&gt;A) occurs at splice donor and may alter <italic>TBX5</italic> gene function by impact on splicing. It was not previously reported in China.</p><p><bold>Conclusion:</bold> Our case reported a novel mutation in <italic>TBX5</italic>, which expanded the known genetic variants associated with HOS.</p>