AUTHOR=García-Ortiz María Victoria , Torre-Aguilar María José de la , Morales-Ruiz Teresa , Gómez-Fernández Antonio , Flores-Rojas Katherine , Gil-Campos Mercedes , Martin-Borreguero Pilar , Ariza Rafael R. , Roldán-Arjona Teresa , Perez-Navero Juan Luis 

TITLE=Analysis of Global and Local DNA Methylation Patterns in Blood Samples of Patients With Autism Spectrum Disorder

JOURNAL=Frontiers in Pediatrics

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/pediatrics/articles/10.3389/fped.2021.685310

DOI=10.3389/fped.2021.685310

ISSN=2296-2360

ABSTRACT=<p>The goal of this investigation was to determine whether there are alterations in DNA methylation patterns in children with autism spectrum disorder (ASD).</p><p><bold>Material and Methods:</bold> Controlled prospective observational case-control study. Within the ASD group, children were sub-classified based on the presence (AMR subgroup) or absence (ANMR subgroup) of neurodevelopmental regression during the first 2 years of life. We analyzed the global levels of DNA methylation, reflected in <italic>LINE-1</italic>, and the local DNA methylation pattern in two candidate genes, Neural Cell Adhesion Molecule (NCAM1) and Nerve Growth Factor (NGF) that, according to our previous studies, might be associated to an increased risk for ASD. For this purpose, we utilized blood samples from pediatric patients with ASD (<italic>n</italic> = 53) and their corresponding controls (<italic>n</italic> = 45).</p><p><bold>Results:</bold> We observed a slight decrease in methylation levels of <italic>LINE-1</italic> in the ASD group, compared to the control group. One of the CpG in <italic>LINE-1</italic> (GenBank accession no.<ext-link ext-link-type="DDBJ/EMBL/GenBank" xlink:href="X58075" xmlns:xlink="http://www.w3.org/1999/xlink">X58075</ext-link>, nucleotide position 329) was the main responsible for such reduction, highly significant in the ASD subgroup of children with AMR (<italic>p</italic> &lt; 0.05). Furthermore, we detected higher <italic>NCAM1</italic> methylation levels in ASD children, compared to healthy children (<italic>p</italic> &lt; 0.001). The data, moreover, showed higher <italic>NGF</italic> methylation levels in the AMR subgroup, compared to the control group and the ANMR subgroup. These results are consistent with our prior study, in which lower plasma levels of <italic>NCAM1</italic> and higher levels of <italic>NGF</italic> were found in the ANMR subgroup, compared to the subgroup that comprised neurotypically developing children.</p><p><bold>Conclusions:</bold> We have provided new clues about the epigenetic changes that occur in ASD, and suggest two potential epigenetic biomarkers that would facilitate the diagnosis of the disorder. We similarly present with evidence of a clear differentiation in DNA methylation between the ASD subgroups, with or without mental regression.</p>