AUTHOR=Alissa Rana , Williams Patty D. , Baker Erika L. , Hipp Jennifer A. , Saremian Jinous , Aysola Agnes E. 

TITLE=Suitability of Placental Blood Samples of Newborns for Pre-Transfusion Testing

JOURNAL=Frontiers in Pediatrics

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/pediatrics/articles/10.3389/fped.2021.661321

DOI=10.3389/fped.2021.661321

ISSN=2296-2360

ABSTRACT=<p><bold>Objective:</bold> To show concordance between heel stick and placental blood sample pairs for newborns' pre-transfusion testing and to validate placental blood's tube and gel methodology.</p><p><bold>Methods:</bold> Placental samples were collected for pre-transfusion testing at birth from 78 singleton and twin newborns admitted to our Mother–Baby Unit to compare with the results of heel stick samples taken from same newborns. Gestational age ≥35 weeks, weight ≥2,000 g. The study was approved by the Institutional Review Board (IRB). Informed consent was obtained from newborn parents. ABO blood group, Rhesus factor (Rh), direct antiglobulin test (DAT), and antibody screen were performed. Ortho ProVue Analyzer was used for tube and gel methods. McNemar's test for paired categorical data was performed.</p><p><bold>Results:</bold> One hundred percent concordance in 78 pairs for ABO and Rh. Seventy-four pairs were tested for antibodies, 72 were both negative, 1 was both positive, and 1 gave discordant result. Ninety-nine percent concordance, <italic>p</italic> = 0.999. Sixty-five pairs were both DAT negative, seven were both DAT positive, and six gave discordant results. Ninety-two percent concordance, <italic>p</italic> = 0.68. Placental blood gave identical results comparing tube with gel methods.</p><p><bold>Conclusions:</bold> Placental blood is suitable for pre-transfusion testing and can replace heel sticks. Placental blood tube and gel methods are validated.</p>