AUTHOR=Hsiao Chien-Chou , Lee Cheng-Han , Yang Rei-Cheng , Chen Jia-Yuh , Su Tzu-Cheng , Chang Yu-Jun , Lin Ching-Yuang , Tsai Yi-Giien 

TITLE=Heat Shock Protein-70 Levels Are Associated With a State of Oxidative Damage in the Development of Bronchopulmonary Dysplasia

JOURNAL=Frontiers in Pediatrics

VOLUME=9

YEAR=2021

URL=https://www.frontiersin.org/journals/pediatrics/articles/10.3389/fped.2021.616452

DOI=10.3389/fped.2021.616452

ISSN=2296-2360

ABSTRACT=<p><bold>Background:</bold> Heat shock protein-70 (Hsp-70) exhibits cytoprotective effects against oxidative stress-induced airway injury. This study aimed to examine Hsp-70 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) from tracheal aspirates (TA) in very low-birth weight (VLBW) preterm infants to predict the development of bronchopulmonary dysplasia (BPD).</p><p><bold>Methods:</bold> This birth cohort study enrolled 109 VLBW preterm infants, including 32 infants who developed BPD. Hsp-70 and 8-OHdG concentrations from TA were measured by immunoassay. The apoptosis of TA epithelial cells obtained on Day 28 after birth was measured using annexin-V staining assay.</p><p><bold>Results:</bold> Hsp-70 and 8-OHdG levels in TA fluid were persistently increased from Day 1 to Day 28 of life in the BPD group. Multiple linear regression analysis demonstrated that BPD was significantly associated with gestational age, respiratory distress syndrome, and TA Hsp-70 and 8-OHdG levels on post-natal Day 28. The TA Hsp-70 level positively correlated with TA 8-OHdG level on the Day 1 (<italic>r</italic> = 0.47) and Day 28 of life (<italic>r</italic> = 0.68). Incubation of recombinant Hsp-70 with primary epithelial cells derived from TA of patients decreased hydrogen peroxide-induced epithelial cell death.</p><p><bold>Conclusions:</bold> Hsp-70 levels are associated with a state of oxidative injury in the development of BPD.</p>