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BRIEF RESEARCH REPORT article
Front. Pain Res.
Sec. Pain Mechanisms
Volume 6 - 2025 | doi: 10.3389/fpain.2025.1492773
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IntroductionChronic pain is a disabling condition that is accompanied by neuropsychiatric comorbidities such as anxiety, depression, and cognitive decline. While the peripheral alterations are well-studied, we lack an understanding of how these peripheral changes can result in long-lasting brain alterations and the ensuing behavioral phenotypes. This study aims to quantify changes in cerebral blood perfusion using laser speckle contrast imaging (LSCI) in the murine Complete Freund’s adjuvant (CFA) model of unilateral peripheral inflammation.MethodsTwenty four adult male and female C57BL/6 mice were randomly assigned to control (0.05ml saline) or 1 of 3 experimental groups receiving CFA (0.01ml, 0.05ml, and 0.1ml) on the right hindpaw. Three days after the intraplantar injections, animals were examined for signs of inflammation and subjected to craniotomy and in vivo LSCI of the parietal-temporal lobes.ResultsUnilateral administration of CFA resulted in signs of local inflammation as well as cerebral hypoperfusion in dose-dependent manner.DiscussionTo our knowledge, this is the first study using laser speckle contrast imaging to examine the effects of CFA-induced peripheral inflammation on cerebral blood perfusion. It serves as a first step in delineating the path by which insult to peripheral tissues can cause long-lasting brain plasticity via vascular mechanisms.
Keywords: Complete Freud's adjuvant, laser speckle contrast imaging, Animal model of pain, cerebral blood flow (CBF), Inflammation
Received: 07 Sep 2024; Accepted: 24 Mar 2025.
Copyright: © 2025 Kazeem, Ge and Tajerian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Maral Tajerian, Queens College (CUNY), New York City, United States
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
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