AUTHOR=Maquera-Huacho Patricia Milagros , Spolidorio Denise Palomari , Manthey John A. , Grenier Daniel 

TITLE=Eriodictyol Suppresses Porphyromonas gingivalis-Induced Reactive Oxygen Species Production by Gingival Keratinocytes and the Inflammatory Response of Macrophages

JOURNAL=Frontiers in Oral Health

VOLUME=3

YEAR=2022

URL=https://www.frontiersin.org/journals/oral-health/articles/10.3389/froh.2022.847914

DOI=10.3389/froh.2022.847914

ISSN=2673-4842

ABSTRACT=<p><italic>Porphyromonas gingivalis</italic> is a key pathogen of periodontitis, an inflammatory disease that affects the tooth-supporting tissues. The aim of the present study was to investigate the effects of the flavanone eriodictyol on <italic>P. gingivalis</italic>-induced reactive oxygen species (ROS) production by gingival keratinocytes and the inflammatory response of macrophages. <italic>Porphyromonas gingivalis</italic> and H<sub>2</sub>O<sub>2</sub> acted synergistically to induce ROS production by keratinocytes. The presence of eriodictyol significantly attenuated ROS production in a dose-dependent manner. We used a macrophage model to show that eriodictyol decreases the secretion of IL-1β, IL-6, IL-8, and TNF-α induced by <italic>P. gingivalis</italic>. Evidence has been brought that this anti-inflammatory property of eriodictyol may be related to its ability to prevent the activation of the NF-κB signaling pathway by <italic>P. gingivalis</italic>. This periodontal pathogen was also found to be a potent inducer of matrix metalloproteinase (MMP) production by macrophages, including MMP-2, MMP-8, and MMP-9. Eriodictyol dose-dependently inhibited the production of all three MMPs. Lastly, eriodictyol inhibited the catalytic activity of both MMP-9 and <italic>P. gingivalis</italic> collagenase. In conclusion, eriodictyol may be a potential therapeutic agent for preventing and/or treating periodontal disease due to its antioxidant, anti-inflammatory, and anti-proteinase properties.</p>