95% of researchers rate our articles as excellent or good
Learn more about the work of our research integrity team to safeguard the quality of each article we publish.
Find out more
ORIGINAL RESEARCH article
Front. Oncol.
Sec. Pharmacology of Anti-Cancer Drugs
Volume 15 - 2025 | doi: 10.3389/fonc.2025.1586344
This article is part of the Research Topic Decoding Tumor Drug Resistance: Machine Learning’s Role from Molecules to Treatment View all 8 articles
Acute Aerobic Exercise Alters Serum Protein Distribution in Colorectal Cancer Patients
Provisionally accepted- 1 First Affiliated Hospital of Harbin Medical University, Harbin, China
- 2 Beidahuang Industry Group General Hospital, Harbin, Heilongjiang Province, China
Background: Acute aerobic exercise has been shown to exert beneficial effects on colorectal cancer (CRC) patients, however, the specific molecular mechanisms underlying these effects remain unclear. To investigate the relationship between exercise and CRC progression, we conducted a prospective cohort study to analyze the impact of acute aerobic exercise on serum protein profiles in CRC patients.Methods: Serum samples from 10 CRC patients were collected and analyzed using proteomics following either no exercise or acute aerobic exercise. Weighted gene coexpression network analysis (WGCNA) was employed to identify protein modules associated with exercise. Protein-protein interaction (PPI) analysis was further conducted to pinpoint key proteins influenced by exercise. Western blotting was used to validate the expression changes of identified proteins.WGCNA revealed that the blue module exhibited the highest correlation with 42 serum protein, 27 of which showed significant changes post-exercise compared with preexercise. PPI analysis identified ARF6, ARF5, and RAB11A as the core proteins.Western blotting further confirmed that their expression levels were significantly reduced in the post-exercise group, making them key targets in current clinical treatment protocols.This study demonstrates that acute aerobic exercise alters the serum protein profile in CRC patients, with significant reductions in ARF6, ARF5, and RAB11A representing the most meaningful changes. These findings provide strong evidence supporting the use of acute aerobic exercise as a therapeutic intervention for CRC.
Keywords: colorectal cancer, Acute aerobic exercise, Proteomics, differential expression analysis, WGCNA
Received: 02 Mar 2025; Accepted: 24 Mar 2025.
Copyright: © 2025 Jin, Han, Wang, Yang and Chen. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Sinan Jin, First Affiliated Hospital of Harbin Medical University, Harbin, China
Chao Chen, Beidahuang Industry Group General Hospital, Harbin, Heilongjiang Province, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.