Jinlong Tian ¹ Yanlei Li ¹ Yu Tong ² Yuan Zhang ³ Tingxiao Zhao ² Yao Kang ² Qing Bi ^1*

• ¹ Bengbu Medical College, Bengbu, Anhui Province, China
• ² Sports Medicine Center, Department of Orthopedics, Zhejiang Provincial People’s Hospital (Affiliated People's Hospital, Hangzhou Medical College), hangzhou, China
• ³ Department of Rheumatology and Immunology, The Second Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine., hangzhou, China

UCK2 is a promising prognostic marker for malignant tumors, but its association with immune infiltration and cancer stemness in pan-cancer remains to be fully understood. we find that gene UCK2 is closed related to RNAss and DNAss, which is measured the tumor stemness. We also discover an association between UCK2 expression and immune cells by CIBERSORT algorithm, ESTIMATE algorithm and ssGSEA algorithm. This study aims to shed light on the role and possible mechanism of UCK2 in pan-cancer. We used the R programming language for pan-cancer bulk sequencing data analysis, which were obtained from UCSC datasets.UCSC database is a very useful for explore data from TCGA and other cancer genomics datasets, The data we explored at the UCK2 transcriptome level came from TCGA data in the UCSC database. We explored differential UCK2 expression between tumor and normal samples. IHC was utilized to validate the expression of UCK2 in different types cancers using tumor tissue chips.The correlations of UCK2 with prognosis, genetic instability, DNA repair, cancer stem cell characteristics, and immune cell infiltration were investigated. Furthermore, single-cell datasets, acquired from GEO database, were used to validate the relationship between UCK2 and immune cells. GEO is a famous public genomics database supporting freely disseminates microarray data.Finally, we analyzed the correlation between UCK2 and drug sensitivity. UCK2 expression was observed to be high in most cancers and was remarkably related to the prognosis of pan-cancers. We found that the increased UCK2 expression was associated with higher genetic instability. Additionally, positive relationships were observed between UCK2 expression and mismatch repair genes, homologous recombination repair genes, and cancer stemness across different cancer types. There were significant correlations between UCK2 and T cells, monocytes, mast cells, and macrophages. Moreover, as expected, the immune checkpoint HLA was found to be negatively related to UCK2. Similarly, UCK2 was also observed to have a negative association with MHC genes. We noted that UCK2 had significant correlations with the sensitivity to various anti-cancer drug. Finally, we have observed that UCK2 plays pivotal roles in prognosis and tumor immunity, and it is associated with DNA repair and cancer stemness.