Jie Ma ¹ Hongtao Wang ¹ Zhengwei Gui ² Yuanrong Yang ^1*

• ¹ Jingzhou Hospital Affiliated to Yangtze University, China, China
• ² Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, China

Although SYNGR4 is recognized as a contributing gene to amyotrophic lateral sclerosis, its involvement in the progression of breast cancer remains unknown. SYNGR4 expression in multiple types of cancer, such as breast cancer. The effect of SYNGR4 on breast cancer prognosis was analyzed using bioinformatics and possible pathways by which this molecule affects breast cancer prognosis were explored. GSVA was used to analyze the impact of SYNGR4 on immune infiltration in breast cancer, while cellular and animal experiments confirmed the influence of SYNGR4 on breast cancer proliferation, migration, and tumor-associated macrophage polarization within cancer foci. SYNGR4 is abundantly present in numerous cancerous growths, such as breast cancer, and has an impact on the outlook for individuals with breast cancer. This may be a volatile effect through Organelle fission, chromosome segregation, nuclear division, etc. Increased SYNGR4 expression impacts the growth, movement, and immune cell infiltration of breast cancer, as well as encourages the transformation of tumor-associated macrophages in breast cancer towards M2 polarization. SYNGR4 overexpression can affect the prognosis of breast cancer patients by promoting M2 polarization of tumorassociated macrophages in breast cancer, and this molecule may be a novel target for breast cancer immunotherapy.