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ORIGINAL RESEARCH article

Front. Oncol.
Sec. Cancer Molecular Targets and Therapeutics
Volume 14 - 2024 | doi: 10.3389/fonc.2024.1485605
This article is part of the Research Topic Therapeutic Mechanism of Osteosarcoma View all 6 articles

Deciphering the Oncogenic Network: How C1QTNF1-AS1 Modulates Osteosarcoma Through miR-34a-5p and Glycolytic Pathways

Provisionally accepted
Yu Zhang Yu Zhang 1Hailong Lun Hailong Lun 2Naiqiang Zhu Naiqiang Zhu 1*Ning Yang Ning Yang 1Kaikai Ding Kaikai Ding 1Bin Chen Bin Chen 1Chengbing Chang Chengbing Chang 1Haipeng Gu Haipeng Gu 1Yanqi Liu Yanqi Liu 1
  • 1 Affiliated Hospital of Chengde Medical University, Chengde, China
  • 2 Tangshan Nanhu Hospital, Tangshan, Hebei Province, China

The final, formatted version of the article will be published soon.

    Osteosarcoma (OS), a metastatic cancer prevalent in youth with grim prognosis, is modulated by long non-coding RNAs (lncRNAs), notably C1QTNF1-AS1, an oncogene associated with multiple tumors. This research focused on elucidating the functional roles and underlying mechanisms of C1QTNF1-AS1 in OS cells.Leveraging bioinformatics, miR-34a-5p was pinpointed as specifically interacting with C1QTNF1-AS1 while also targeting LDHA and PDK3, validated via dualluciferase assays and RNA immunoprecipitation. Expression analysis using qRT-PCR and Western blot in OS cells revealed intricate interplay among C1QTNF1-AS1, miR-34a-5p, LDHA, and PDK3. Functional evaluations demonstrated that C1QTNF1-AS1 silencing upregulated LDHA and PDK3, partially offset by miR-34a-5p mimics.Inhibition of C1QTNF1-AS1 accelerated OS cell proliferation, motility, invasion, and the Warburg effect, whereas miR-34a-5p overexpression alleviated these effects. In summary, our findings underscore C1QTNF1-AS1's pivotal role in OS pathogenesis.Its downregulation indirectly upregulates LDHA and PDK3 through miR-34a-5p suppression, thereby fostering OS progression by enhancing glycolytic metabolism and cancer cell behaviors including growth, migration, and invasion.

    Keywords: os, C1QTNF1-AS1, miR-34a-5p, LdhA, PDK3, Warburg effect

    Received: 24 Aug 2024; Accepted: 18 Dec 2024.

    Copyright: © 2024 Zhang, Lun, Zhu, Yang, Ding, Chen, Chang, Gu and Liu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence: Naiqiang Zhu, Affiliated Hospital of Chengde Medical University, Chengde, China

    Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.